Poly(I:C) combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs

Cao, Yimei; Lu, Zengjun; Li, Yanli; Sun, Pu; Li, Dong; Li, Pinghua; Bai, Xingwen; Fu, Yuanfang; Bao, Huang; Zhou, Chun-Xue; Xie, Baoxia; Chen, Yingli; Liu, Zaixin

doi:10.1016/j.antiviral.2012.11.009

Cited by 45 publications

(34 citation statements)

References 37 publications

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“…At 28 days post-vaccination (dpv), all pigs were challenged intramuscularly in the neck with 1000 ID 50 (50% pig infectious doses) of the WT virus. This challenge model of FMDV has been well developed for the FMD vaccine potency test in LVRI [25], and also has been used as a standard method for detecting potency of all commercially inactivated vaccines for pigs in China [26]. The animals were examined daily for fever and clinical signs.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Bai

et al. 2014

Vet Res

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Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease of cloven-hoofed animals in the world. The disease can be effectively controlled by vaccination of susceptible animals with the conventional inactivated vaccine. However, one major concern of the inactivated FMD virus (FMDV) vaccine is that it does not allow serological discrimination between infected and vaccinated animals, and therefore interferes with serologic surveillance and the epidemiology of disease. A marker vaccine has proven to be of great value in disease eradication and control programs. In this study, we constructed a marker FMDV containing a deletion of residues 93 to 143 in the nonstructural protein 3A using a recently developed FMDV infectious cDNA clone. The marker virus, r-HN/3A93–143, had similar growth kinetics as the wild type virus in culture cell and caused a symptomatic infection in pigs. Pigs immunized with chemically inactivated marker vaccine were fully protected from the wild type virus challenge, and the potency of this marker vaccine was 10 PD50 (50% pig protective dose) per dose, indicating it could be an efficacious vaccine against FMDV. In addition, we developed a blocking ELISA targeted to the deleted epitope that could clearly differentiate animals infected with the marker virus from those infected with the wild type virus. These results indicate that a marker FMDV vaccine can be potentially developed by deleting an immunodominant epitope in NSP 3A.

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Section: Methodsmentioning

confidence: 99%

Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Bai

et al. 2014

Vet Res

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“…Recently, it was shown to reduce, by 80-fold, the dose required for protection of a recombinant adenovirus expressing FMDV A24 capsids [(50); Table 2]. Another synthetic analog of dsRNA, polyinosinic-polycytidylic acid (poly IC), potentiated the protection afforded by a multi-epitope vaccine in pigs (66). This vaccine incorporated linked B cell epitopes (the G–H loop and C terminus of VP1) from four topotypes of serotype O flanked by two universal T cell epitopes.…”

Section: Novel Approaches To Vaccines and Vaccinationmentioning

confidence: 99%

Challenges of Generating and Maintaining Protective Vaccine-Induced Immune Responses for Foot-and-Mouth Disease Virus in Pigs

et al. 2016

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Vaccination can play a central role in the control of outbreaks of foot-and-mouth disease (FMD) by reducing both the impact of clinical disease and the extent of virus transmission between susceptible animals. Recent incursions of exotic FMD virus lineages into several East Asian countries have highlighted the difficulties of generating and maintaining an adequate immune response in vaccinated pigs. Factors that impact vaccine performance include (i) the potency, antigenic payload, and formulation of a vaccine; (ii) the antigenic match between the vaccine and the heterologous circulating field strain; and (iii) the regime (timing, frequency, and herd-level coverage) used to administer the vaccine. This review collates data from studies that have evaluated the performance of foot-and-mouth disease virus vaccines at the individual and population level in pigs and identifies research priorities that could provide new insights to improve vaccination in the future.

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“…Polyinosinic: polycytidylic acid (poly I:C) is a synthetic viral double-stranded RNA (dsRNA) analog. Previous studies in human and other species have demonstrated that it can mimic viral infection, and has stimulatory effects similar to viral dsRNA [15,16,17]. Using poly I:C as an immunologic stimulant, gene transcriptome analyses of PBMC or whole blood have made tremendous progress in humans as well as in pigs [18,19,20].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Transcriptome of Poly I:C-Stimulated Peripheral Blood Mononuclear Cells Reveals Evidence for MicroRNAs in Regulating Host Response to RNA Viruses in Pigs

Wang

et al. 2016

IJMS

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MicroRNAs (miRNAs) are one family of small noncoding RNAs that function to modulate the activity of specific mRNA targets in animals. To understand the role of miRNAs in regulating genes involved in the host immune response to RNA viruses, we profiled and characterized the miRNAs of swine peripheral blood mononuclear cells (PBMC) stimulated with poly I:C, a synthetic dsRNA analog, by miRNA-sequencing (miRNA-seq). We identified a total of 905 miRNAs, of which 503 miRNAs were firstly exploited herein with no annotation in the latest miRBase 21.0. Expression analysis demonstrated that poly I:C stimulation can elicit significantly differentially expressed (DE) miRNAs in Dapulian (n = 20), one Chinese indigenous breed, as well as Landrace (n = 23). By integrating the mRNA expression profiles of the same sample with miRNA profiles, we carried out function analyses of the target genes of these DE miRNAs, with the results indicating that target genes were most enriched in some immune-related pathways and gene ontology (GO) terms, suggesting that DE miRNAs play an important role in the regulation of host to poly I:C stimulation. Furthermore, we also detected 43 and 61 significantly DE miRNAs between the two breeds in the control sample groups and poly I:C stimulation groups, respectively, which may be involved in regulation of the different characteristics of the two breeds. This study describes for the first time the PBMC miRNA transcriptomic response to poly I:C stimulation in pigs, which not only contributes to a broad view of the pig miRNAome but improves our understanding of miRNA function in regulating host immune response to RNA viruses.

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Poly(I:C) combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs

Cited by 45 publications

References 37 publications

Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Challenges of Generating and Maintaining Protective Vaccine-Induced Immune Responses for Foot-and-Mouth Disease Virus in Pigs

MicroRNA Transcriptome of Poly I:C-Stimulated Peripheral Blood Mononuclear Cells Reveals Evidence for MicroRNAs in Regulating Host Response to RNA Viruses in Pigs

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