Poly(styrene–divinylbenzene) beads surface functionalized with di-block polymer grafting and multi-modal ligand attachment: performance of reversibly immobilized lipase in ester synthesis

Abstract

Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface-initiated atom transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand attached beads were used for reversible immobilization of lipase. The influences of pH, ionic strength, and initial lipase concentration on the immobilization capacities of the beads have been investigated. Lipase adsorption capacity of the beads was about 78.1 mg/g beads at pH 6.0. The Km value for immobilized lipase was about 2.1-fold higher than that of free enzyme. The thermal, and storage stability of the immobilized lipase also was increased compared to the native lipase. It was observed that the same support enzyme could be repeatedly used for immobilization of lipase after regeneration without significant loss in adsorption capacity or enzyme activity. A lipase from Mucor miehei immobilized on styrene-divinylbenzene copolymer was used to catalyze the direct esterification of butyl alcohol and butyric acid.

Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres

“…Therefore, many researchers focused on covalent binding of lipase molecules with the functional groups of carriers to obtain stable enzyme performance [21][22][23]. The functional groups of carriers mainly include epoxy [24,25], amide [26], and aldehyde group [27]. It has been reported that lipase was immobilized on macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [poly(GMA-co-EDMA)] resins by covalent binding [28].…”

Synthesis and characterization of magnetic beads containing aminated fibrous surfaces for removal of Reactive Green 19 dye: kinetics and thermodynamic parameters

“…Much attention has been paid to improving the adsorptive performance of the adsorbent, with the main effort being focused on the introduction of a second functional polymer via grafting 2, 14–16. Among the surface functionalization techniques, nano‐fibril polymers can be grafted on the adsorbent and the grafted polymers can be readily modified 17–19. For example, epoxy groups can be readily modified to amino groups by reaction with ammonia 19.…”

Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99–125 lipase via adsorption

“…In order to enhance their reusability, operational stability and recovery, enzymes are immobilized on different carriers [6][7][8][9][10][11][12][13][14]. An important area of interest is the immobilization of lipase on magnetic materials.…”