Polyacrylic resins regulate transcriptional control of interleukin-6, gp80, and gp130 genes in human gingival fibroblasts

Borelli, Bruna; Zarone, Fernando; Rivieccio, Vincenzo; Riccitiello, Francesco; Simeone, Mariarosaria; Sorrentino, R.; Rengo, Sandro; Spagnuolo, Gianrico; Procino, Alfredo

doi:10.2334/josnusd.16-0388

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…The polymerization and conversion of monomer in organic matrix of resin‐based adhesive materials is rarely complete and this seems to be responsible for most of reported adverse effects such as cytotoxicity, allergy and inflammatory potential (Borelli et al, 2017; Goldberg, 2008).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Bationo

Rouamba

Diarra

et al. 2020

Clinical & Exp Dental Res

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Introduction The aim of this study was to determine the cytotoxicity of light‐cured composite resins (Clearfil ES‐2, Clearfil ES Flow, Filtek Supreme XTE, Grengloo, Blugloo, Transbond XT, and Transbond LR) then to assess leachable components in contact with human gingival fibroblasts (GFs) and to quantity detected bisphenol A (BPA). Methods Light‐cured composite resin discs were immersed for 24 hours in gingival fibroblastic medium (n = 3 for each product) and in control medium (n = 2 for each product) contained in plate. Cytotoxicity of the products (n = 95) was determined by the measure of cell viability using MTT assay after reading the optical densities of the plates. The analysis of leachable components was done by gas phase chromatography and mass spectrometry (GC–MS) and detected BPA was quantified. The limit of quantification was 0.01 μg/mL. Statistical analyses were performed by using IBM SPSS Statistics 20 and Kruskal–Wallis and Mann–Whitney U‐tests were applied. Results Cell viabilities were between 85 and 90%. Many chemical compounds including triethylene glycol dimethacrylate (TEGDMA) and BPA were identified. The average concentrations were 0.67 μg/mL ± 0.84 in the control medium and 0.73 μg/mL ± 1.05 in the fibroblastic medium. Filtek Supreme XTE presented the highest concentration of BPA with 2.16 μg/mL ± 0.65 and Clearfil ES Flow presented the lowest with 0.25 μg/mL ± 0.35. No BPA was detected with Transbond XT and Transbond LR. Clearfil ES Flow, Filtek Supreme XTE, Grengloo and Transbond LR presented residual TEGDMA. Conclusions Light‐cured composite resins are slightly cytotoxic opposite GFs and release many components including BPA and TEGDMA. Clinical precautions should be taken to decrease the release of these monomers.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Bationo

Rouamba

Diarra

et al. 2020

Clinical & Exp Dental Res

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“…According to the strategies for material testing, presented by Wataha (2012), the purpose of our study, was to determine in vitro material liability, the dynamics of any component release and the potential of released monomers to alter cell metabolism and function. Polymerization and monomer conversion of the RBDM organic matrix is rarely fully completed and, this event seems to be responsible for most of the reported undesirable effects such as cytotoxicity, allergic and inflammatory potential (Goldberg, 2008;Borelli et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of Orthodontic Resins: <i>In vitro</i> Evaluation of Monomer Leaching and Cytotoxic Effects

Uomo

Spagnuolo²,

Nocca

et al. 2017

Current Research in Dentistry

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Abstract:The aim of this study was to investigate the effect of orthodontic resins on cell survival and to evaluate monomer leaching both before and after resin polymerization. Materials and methods: 3T3 mouse fibroblasts were exposed to three cured and uncured orthodontic resins. Cellular viability was assessed by Alamar Blue assay after 24, 48 and 72 h. High Performance Liquid Chromatography was used to measure the amount of monomers released by the tested samples. Data were analyzed by means of ANOVA and Tukey's test (p<0.05). All tested materials exerted a cytotoxic response. Cytotoxicity tests showed that the uncured samples were more cytotoxic than the polymerized ones. A time-dependent reduction in cellular viability was found. Monomer release analyses indicated a higher elution of Triethylene Glycol Dimethacrylate (TEGDMA) compared to Bisphenol A Glycidyl Methacrylate. TEGDMA release was higher in the uncured samples and showed a time-dependent pattern. Our results showed the role of resin curing in determining the cytotoxic effect of orthodontic resins and suggested that the differences in the chemical composition of resin matrix appeared to be much more related to the decrease in cell viability than the amount of monomer leaching from orthodontic resins. Clinicians should pay greater attention to resin curing after bracket placement in order to reduce the potentially dangerous effect of monomer release.

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“…Despite its advantages, adverse reactions to PMMA have been reported in the literature [ 10 – 12 ]. The viability of cells exposed to the acrylic resin is influenced mainly by the presence of residual monomers in the samples, that is, by the degree of conversion (DC) achieved in the polymerization reaction [ 9 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Pressure, Post-Pressing Time, and Polymerization Cycle on the Degree of Conversion of Thermoactivated Acrylic Resin

Leão

Moraes

Aquino

et al. 2018

International Journal of Dentistry

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Herein, the effect of different post-pressing times and pressure in two cycles of polymerization on the degree of conversion (DC) of thermally activated acrylic resin (TRRA) is analyzed to optimize the polymerization of this material. After post-pressing for 0, 6, or 12 h, polymerization was performed with or without a pressure of 60 psi (0.41 MPa) in a short (4 h) or a long (11 h) cycle, totaling 12 groups. To determine the DC, PMMA specimens were analyzed by Fourier transform infrared spectroscopy. The influence of each factor alone on the DC was studied by experimental planning. The statistical tests used were three-way ANOVA, t-test, Tukey's test, and Levene's test, with a margin of error of 5%. Two groups prepared with post-pressing times of 12 h had the lowest DC (p < 0.001). Post-pressing times of 0 and 6 h did not yield statistically different results. Pressure increased the DC in only one group (long cycle +12 h, p=0.001). The short cycle resulted in a higher DC than the long cycle in 2 groups (with pressure +0 h, p=0.002; without pressure +6 h, p=0.015), while the long cycle yielded a statistically higher DC in only one group (with pressure +12 h, p < 0.001). The polymerization showed satisfactory DC in all 12 groups. Small differences found among the specimens indicate that the pressure, post-pressing time, and polymerization cycles herein were not influential factors for the DC of PMMA.

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Polyacrylic resins regulate transcriptional control of interleukin-6, gp80, and gp130 genes in human gingival fibroblasts

Cited by 12 publications

References 21 publications

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Biocompatibility of Orthodontic Resins: <i>In vitro</i> Evaluation of Monomer Leaching and Cytotoxic Effects

Effect of Pressure, Post-Pressing Time, and Polymerization Cycle on the Degree of Conversion of Thermoactivated Acrylic Resin

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