Polyadenylated messenger RNA isolated from cells of Rhodopseudomonas capsulata induced to synthesize the photosynthetic apparatus

Yu, Pak-Lam

doi:10.1016/0378-1097(82)90212-9

Cited by 3 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Omission of heparin and diethyl pyrocarbonate in the extraction buffer used for cell disruption reduced the yield of total nucleic acid from this procaryote by 10-fold. The present recovery of oligo(dT)-cellulose-retained RNA of 9% after extraction by phenol-SDS is somewhat lower than values of 20.5 and 15% reported for Bacillus subtilus and E. coli (18,38), respectively, but within the range of 1 to 20.5% isolated from various bacteria (12,18,21,27,38,46) and spinach chloroplasts (43). The RNA fraction subjected to fractionation upon oligo(dT)cellulose was free of high-and low-molecular-weight sheared 11.2 Second elution: recycling of unbound fraction from first 10.0 elution Third elution: recycling of unbound fraction from the 9.2 second elution Fourth elution with high salt: 15 mM Tris-chloride (pH 9.0 7.5) containing 0.5 M NaCl Fifth elution with lower salt: 15 mM Tris-chloride (pH 8.9 7.5) containing 0.12 M NaCl Sixth elution with sterile water 0 a Before chromatography the sample was dissolved in 2.0 ml of water, heated to 73°C for 15 min, cooled to 23°C, and adjusted (in 5.0 ml) to 15 mM Tris-chloride (pH 7.5), 1 mM EDTA, 0.5 M NaCl, and 0.3% lithium dodecyl sulfate.…”

Section: Resultscontrasting

confidence: 70%

“…1) suggest that poly(A)+ mRNAs in R. rubrum also follow this trend. By contrast, only one poly(A)+ RNA of 80,000 daltons, migrating faster than 16S rRNA in 6% formaldehyde gel, has been reported to occur in induced cells of Rhodopseudomonas capsulata (46). The length of poly(A)+ fragments in non-photosynthetic bacteria varies from 15 to 80 nucleotide residues (11,25,27,33,38).…”

Section: Discussionmentioning

confidence: 97%

“…The priming of cell-free systems, derived either from eucaryotic wheat germ or E. coli, by poly(A)+ RNAs purified from photosynthetic (21,46) or non-photosynthetic (11) bacteria results in enhanced incorporation of radiolabeled amino acids into peptides. The present research establishes that this enhancement is 220-fold greater (on a unit mass basis) than that observed with poly(A)-RNA.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Majumdar¹,

McFadden²

1984

J Bacteriol

View full text Add to dashboard Cite

Total cellular RNA extracted fromh Rhodospirillum rubrum cultured in butyrate-containing medium under strict photosynthetic conditions to the stationary phase of growth has been fractionated on an oligodeoxythymidylic acid-cellulose column into polyadenylated [poly(A)'] RNA and poly(A)-RNA fractions. The poly(A)+ fraction was 9 to 10% of the total bulk RNA isolated. Atnalysis of the poly(A)+ RNA on a denaturing urea-polyacrylamide gel revealed four sharp bands of RNA distributed in heterodisperse fashion between 16S and 9S. Similar fractionation of the poly(A)-RNA resulted in the separation of 23, 16, and 5S rRNAs and 4S tRNA. Poly(A)+ fragments isolated after combined digestion with pancreatic A and T1 RNases and analysis by denaturing gel electrophoresis demonstrated two major components of 80 and 100 residues. Alkaline hydrolysis of the nuclease-resistant, purified residues showed AMP-rich nucleotides. Through the use of snake venom phosphodiesterase, poly(A) tracts were placed at the 3' end of poly(A)+ RNA. Stimtlation of [3H]leucine incorporation into hot trichloroacetic acid-precipitable polypeptides in a cell-free system from wheat germ primed by the poly(A)+ RNA mixture was found to be 220-fold higher than that for poly(A)-RNAs (on a unit mass basis), a finding which demonstrated that poly(A)+ RNAs in R. rubrum are mRNAs. Gel electrophoretic analysis of the translation mixture revealed numerous 3H-labeled products including a major band (Mr, 52,000). The parent protein was precipitated by antibodies to ribulose bisphosphate carboxylase-oxygenase and comprised 6.5% of the total translation products.

show abstract

Section: Resultscontrasting

confidence: 70%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Majumdar¹,

McFadden²

1984

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…In contrast to eucaryotic poly(A)+ RNAs, eubacterial poly(A)+ RNAs have much shorter poly(A) tracts, are unstable, exhibit a wide range of sizes, and comprise a much smaller percentage of the total cellular RNA (6,12,19,30,37,41). In several cases, eubacterial poly(A)+ RNAs have been shown by in vitro translation to be functional mRNAs (30,31,45).…”

mentioning

confidence: 99%

Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii

Brown¹,

Reeve²

1985

J Bacteriol

View full text Add to dashboard Cite

RNA molecules have been isolated from Methanococcus vannielii by oligodeoxythymidylate-cellulose affinity chromatography at 4C. Approximately 16% of the label in RNA isolated from cultures allowed to incorporate [3H]uridine for 3 min at 37C was poly(A)+ RNA. In contrast, less than 1% of the radioactivity in RNA labeled over a period of several generations was contained in poly(A)+ RNA molecules. Electrophoretic separation of poly(A)+ RNA molecules showed a heterogeneous population with mobilities indicative of sizes ranging from 900 to 3,000 bases in length. The population of poly(A)+ RNA molecules was found to have a half-life in vivo of approximately 12 min. Polyadenylate [poly(A)] tracts were isolated by digestion with RNase A and RNase T, after 3' end labeling of the poly(A)+ RNA with RNA ligase.

show abstract

POLYADENYLATION OF mRNA IN PROKARYOTES

Sarkar

1997

Annu. Rev. Biochem.

207

169

View full text Add to dashboard Cite

The 3'-ends of both prokaryotic and eukaryotic mRNA are polyadenylated, but the poly(A) tracts of prokaryotic mRNA are generally shorter, ranging from 15 to 60 adenylate residues and associated with only 2-60% of the molecules of a given mRNA species. The sites of polyadenylation of bacterial mRNA are diverse and include the 3'-ends of primary transcripts, the sites of endonucleolytic processing in the 3' untranslated and intercistronic regions, and sites within the coding regions of mRNA degradation products. The diversity of polyadenylation sites suggests that mRNA polyadenylation in prokaryotes is a relatively indiscriminate process that can occur at all mRNA's 3'-ends and does not require specific consensus sequences as in eukaryotes. Two poly(A) polymerases have been identified in Escherichia coli. They are encoded by unlinked genes, neither of which is essential for growth, suggesting significant functional overlap. Polyadenylation promotes the degradation of a regulatory RNA that inhibits the replication of bacterial plasmids and may play a similar role in the degradation of mRNA. However, under certain conditions, poly(A) tracts may lead to mRNA stabilization. Their ability to bind S1 ribosomal protein suggests that poly(A) tracts may also play a role in mRNA translation.

show abstract

Polyadenylated messenger RNA isolated from cells of Rhodopseudomonas capsulata induced to synthesize the photosynthetic apparatus

Cited by 3 publications

References 10 publications

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Polyadenylated, noncapped RNA from the archaebacterium Methanococcus vannielii

POLYADENYLATION OF mRNA IN PROKARYOTES

Contact Info

Product

Resources

About