Polyamine binding to phospholipid vesicles and inhibition of lipid peroxidation

Tadolini, Bruna; Cabrini, Luciana; Landi, Laura; Varani, Elisabetta; Pasquali, P.

doi:10.1016/s0006-291x(84)80068-6

Cited by 67 publications

(40 citation statements)

References 14 publications

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“…An apparently important consequence of the binding of polyamines to the polar head-groups of the membranes is their protective effect against lipid peroxidation (Kitada et al, 1979;Tadolini et al, 1985b). In model systems, in order to observe an inhibition of fatty acid peroxidation induced by Fenton's reagent, the formation of a complex between the polyamines and the phosphate head-group of acidic unsaturated phospholipids is of paramount importance Tadolini, 1988).…”

Section: Interaction Of Polyamines With Membrane Componentsmentioning

confidence: 99%

Influence of polyamines on membrane functions

Schuber

1989

Biochemical Journal

421

222

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Section: Interaction Of Polyamines With Membrane Componentsmentioning

confidence: 99%

Influence of polyamines on membrane functions

Schuber

1989

Biochemical Journal

421

222

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“…The NADPH-dependent superoxide generation rate in chilled leaves changed with time in close parallel to the NADPH oxidation rate. It is known that PAs are capable of protecting membranes against ROSinduced lipid peroxidation (Kitada et al, 1979;Tadolini et al, 1984;Tadolini, 1988). Ogata et al (1996) recently found that PAs inhibited the activity of the superoxide-generating NADPH oxidases in human neutrophils.…”

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confidence: 99%

Involvement of Polyamines in the Chilling Tolerance of Cucumber Cultivars

2000

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The possible involvement of polyamines (PAs) in the chilling tolerance of cucumber (Cucumis sativus L. cv Jinchun No. 3 and cv Suyo) was investigated. Plants with the first expanded leaves were exposed to 3°C or 15°C in the dark for 24 h (chilling), and then transferred to 28°C/22°C under a 12-h photoperiod for another 24 h (rewarming). Chilling-tolerant cv Jinchun No. 3 showed a marked increase of free spermidine (Spd) in leaves, once during chilling and again during rewarming. Putrescine increased significantly during rewarming, but the increase of spermine was slight. Any of these PAs did not increase in chilling-sensitive cv Suyo during either period. PA-biosynthetic enzyme activities appear to mediate these differences between cultivars. Pretreatment of Spd to cv Suyo prevented chill-induced increases in the contents of hydrogen peroxide in leaves and activities of NADPH oxidases and NADPH-dependent superoxide generation in microsomes and alleviated chilling injury. Pretreatment of methylglyoxal-bis-(guanylhydrazone), a PA biosynthesis inhibitor, to chilled cv Jinchun No. 3 prevented Spd increase and enhanced microsomal NADPH oxidase activity and chilling injury. The results suggest that Spd plays important roles in chilling tolerance of cucumber, probably through prevention of chill-induced activation of NADPH oxidases in microsomes.

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“…Benavides et al [7] described that lipid peroxidation induced by paraquat was almost completely prevented by SPM in sunflower leaf discs. Also, Kitada et al [26] reported that SPM was the most effective in inhibiting lipid peroxidation in rat liver microsomes due to direct binding to microsomal phospholipids, and tadolini et al [36] attributed the inhibition of lipid peroxidation by sPm and sPd to the formation of a polycation/phospholipid vesicle complex. the more pronounced protective effect of sPm, in comparison with sPd and Put, could be accounted for its longer chain and greater number of positive charges which result in a more effective neutralizing and membrane stabilizing ability [41].…”

Section: Discussionmentioning

confidence: 99%

Pretreatment with polyamines alleviate the deleterious effects of diuron in maize leaves

Durmus¹,

Bekircan²

2015

Acta Biologica Hungarica

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the effects of diuron, a photosystem ii inhibiting herbicide, on lipid peroxidation, photosynthetic pigments, soluble protein, proline contents and some antioxidant enzymes in maize leaves were studied and protective effects of polyamines against diuron toxicity were investigated. Diuron significantly increased lipid peroxidation, suggesting oxidative damage in the plants and proline content, while it decreased total chlorophyll, carotenoid and soluble protein levels in the leaves during the experiments. Pretreatment with polyamines statistically decreased lipid peroxidation induced by diuron and spermine (sPm) proved to be the most effective polyamine. Also, pretreatment with polyamines significantly prevented the losses of total chlorophyll, carotenoid and soluble protein induced by diuron. on the other hand, pretreatment with polyamines significantly increased proline contents of the leaves in comparison with the leaves treated with diuron. superoxide dismutase (sod), guaiacol peroxidase (GPX) and glutathione reductase (Gr) activities increased in the leaves treated with diuron while catalase (cat) activity decreased. Pretreatment with spermidine (SPD) did not change significantly SOD activity at 24 and 72 hrs of diuron treatment but prevented the increase in sod activity induced by diuron at 48 h. however, pretreatment with sPd increased GPX activity at 24 h and GR activity at 48 and 72 hrs. CAT activity in the leaves pretreated with SPD was similar to that of the leaves treated with diuron. Pretreatment with sPm prevented the increase in sod activity induced by diuron at 48 h but significantly increased it at 72 h of diuron treatment. However, pretreatment with SPM did not significantly change GPX and GR activities during the experiments but reversed the decrease in CAT activity induced by diuron at 72 h. Pretreatment with putrescine (PUT) prevented the increase in SOD activity induced by diuron at 48 and 72 hrs while it increased GPX and GR activities at 48 h of diuron treatment. Also, the decrease in CAT activity induced by diuron at 72 h was completely prevented by Put. it can be concluded that pretreatment of maize leaves with polyamines reduced the damage produced by diuron and the protective effects of polyamines against diuron toxicity were closely associated with antioxidant system.

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Polyamine binding to phospholipid vesicles and inhibition of lipid peroxidation

Cited by 67 publications

References 14 publications

Influence of polyamines on membrane functions

Influence of polyamines on membrane functions

Involvement of Polyamines in the Chilling Tolerance of Cucumber Cultivars

Pretreatment with polyamines alleviate the deleterious effects of diuron in maize leaves

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