Polyamines as markers of malignancy

Bachrach, Uriel

doi:10.1007/978-3-0348-7144-0_2

Cited by 18 publications

(15 citation statements)

References 87 publications

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“…Rapid tumor growth has been associated with remarkable elevation of polyamine biosynthesis and accumulation [4], which leads to higher concentrations of polyamines in urine or serum of almost all cancer patients [4][5][6][7][8][9][10]. Despite of the limitations of polyamines as markers for malignant tumors [11,12], polyamines now are still considered as one group of the tumor markers in humans (although not as a sole marker) and as tracers for evaluating the effectiveness of anticancer drugs [13,14].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Liu

Chen

2004

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Liu

Chen

2004

Journal of Chromatography B

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“…Based on the report by Russell (24), numerous attempts were made to detect urinary polyamines for cancer diagnosis and therapy (25). It soon turned out that polyamines are excreted from tissues and cells as acetyl derivatives and therefore urine samples should be hydrolyzed, chemically or enzymatically, to yield free polyamines.…”

Section: Introductionmentioning

confidence: 99%

Polyamines and carcinogenesis

Bachrach¹

2012

Acta Facultatis Medicae Naissensis

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The naturally occurring polyamines, spermine, spermidine and the diamine putrescine are widespread in nature. They have been implicated in growth and differentiation processes. In 1967, we reported that cancer cells are rich in polyamines. Subsequently, it has been shown that polyamines are released from cancer cells and may be detected in body fluids such as urine, blood and cerebrospinal fluids. It has also been demonstrated that the increase in cellular polyamine levels is an early and an obligatory event in the process of malignant transformation. This increase in cellular polyamine concentration is due to the activation of ornithine decarboxylase (ODC), which catalyses the rate limiting step in polyamine synthesis by converting ornithine to putrescine. Assays of urinary and blood polyamines have been used to detect cancer and to determine the success of therapy. A sensitive, rapid, chemiluminescence-based method for the determination of diamines and polyamines was developed and 2.000 urine samples were tested. An interesting "gene therapy" system for injecting amine oxidases into normal and transformed cells was developed as follows: serum amine oxidase and porcine kidney damine oxidase were trapped within reconstituted Sendai virus envelopes. Chick or rat fibroblasts, transformed by Rous sarcoma virus, were more susceptible to the injected enzymes, compared to the normal culture, when macromolecular synthesis was tested. An in vitro chemosensitivity assay for the testing of the sensitivity of cancer cells from individual patients ("tailored treatment") was also developed. All these studies stress the importance of polyamines in carcinogenesis.

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“…A previous study indicated that the amount of polyamines excreted in the urine was higher in patients with cancer compared to that in healthy individuals (3). However, results of subsequent studies showed that the total amount, as well as the amount of individual free and monoacetylated polyamines in the urine, were not suitable as reliable tumor markers, as these yielded a number of false positive and negative results (4,5). N 1 ,N 12 -diacetylspermine (DiAcSpm) is a minor component of human urine that constitutes less than 0.5% of the total polyamine species in normal human urine.…”

Section: Introductionmentioning

confidence: 99%

The clinical usefulness of urinary N1,N12-diacetylspermine (DiAcSpm) levels as a tumor marker in patients with colorectal cancer

et al. 2012

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Abstract. The aim of this study was to evaluate the usefulness of urinary N 1 ,N 12 -diacetylspermine (DiAcSpm) measured by the colloidal gold aggregation method as a tumor marker for colorectal cancer (CRC). The preoperative urine of 113 CRC patients was collected, and the urinary DiAcSpm was measured by a reagent kit for DiAcSpm determination based on colloidal gold aggregation using automatic biochemical analyzers. The urinary DiAcSpm levels significantly correlated with distant metastasis and Tumor-Node-Metastasis (TNM) stage. The positive rates of urinary DiAcSpm were significantly higher than those of serum carcinoembryonic antigen (CEA) or cancer antigen 19-9 (CA19-9) in stages 0+Ⅰ, II, III and IV. The positive rates of urinary DiAcSpm were also significantly higher than those of serum CEA or CA19-9 in the early and advanced CRC groups according to the Japan Classification of Colorectal Cancer. Therefore, urinary DiAcSpm, measured by a reagent kit for DiAcSpm determination based on colloidal gold aggregation, may be useful as a non-invasive tumor marker in patients with CRC.

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Polyamines as markers of malignancy

Cited by 18 publications

References 87 publications

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection

Polyamines and carcinogenesis

The clinical usefulness of urinary N1,N12-diacetylspermine (DiAcSpm) levels as a tumor marker in patients with colorectal cancer

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