Polyamines in rapid growth and cancer

Jänne, Juhani; Pösö, Hannu; Raina, Aarne

doi:10.1016/0304-419x(78)90015-x

Cited by 702 publications

(419 citation statements)

References 221 publications

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“…There is, however, a variability in the ability of the tumour lines to proliferate under such nutritional conditions. It has been shown previously (Tisdale, 1980) (Mudd & Cantoni, 1969;Cantoni, 1975) and as the source of aminopropyl groups in polyamine biosynthesis (Janne et al, 1978). An increased tRNA methylase activity has been found in several experimental and human tumours (Baguley & Stahelin, 1968).…”

Section: Discussionmentioning

confidence: 90%

“…An increased tRNA methylase activity has been found in several experimental and human tumours (Baguley & Stahelin, 1968). Also an increase in polyamine biosynthesis is associated with the neoplastic state (Janne et al, 1978). This increased requirement presumably accounts for the high levels of SAM in whiteblood-cell preparations from patients with chronic myeloid leukaemia compared with normal peripheral white cells or tboracicduct lymphocytes (Baldessarini & Carbone, 1965 (Caboche & Bachellerie, 1977).…”

Section: Discussionmentioning

confidence: 99%

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Effect of methionine deprivation on methylation and synthesis of macromolecules

Tisdale¹

1980

Br J Cancer

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Summary.-The growth of 4 tumour-cell lines (Walker rat mammary carcinoma (W-256), a mouse lymphoma (TLX5), a mouse bladder carcinoma (MB) and a human bladder carcinoma (EJ)) was much reduced when methionine in the culture medium was substituted by homocysteine. In contrast, a human embryonic fibroblast line grew equally well under such conditions. Although homocysteine alone was unable to support growth of W-256 it stimulated growth at low methionine concentrations.When W-256 was cultured for 24 h in medium containing homocysteine only, the extent of methylation of nucleic acids and the acid-soluble pool of methionine were decreased. However, under such conditions there was an increased methylase activity towards both endogenous substrate and E. coli tRNA. The effect of methionine removal was to cause a large increase in the Vmax value for methylation of tRNA, without any change in the Km value towards S-adenosyl-L-methionine (SAM). For both W-256 and TLX5, methionine deprivation caused a rapid inhibition of RNA biosynthesis, followed by inhibition of DNA synthesis, while protein synthesis tended to increase. This suggests that the inability of W-256 and TLX5 to survive and grow in methionine-deficient, homocysteine-supplemented medium is not due to insufficient methionine for protein biosynthesis, but may be related to an enhanced methylating activity of some tumour-cell lines.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effect of methionine deprivation on methylation and synthesis of macromolecules

Tisdale¹

1980

Br J Cancer

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“…However, in the present study, the potencies of E2 and CEs to affect ODC activity hardly differed between the s.c. and i.u.-injections. The basal activity of ODC is generally low (13), and it was activated by the vehicle alone in R (Fig. 1B).…”

mentioning

confidence: 94%

A Comparison of the Effects of Estradiol and 2- and 4-Hydroxyestradiol on Uterine Ornithine Decarboxylase Activity in Immature Rats

Kuroda

Johnson

Dey

et al. 1993

Japanese Journal of Pharmacology

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“…THE LEVELS OF POLYAMINES and the activities of the enzymes involved in their biosynthesis increase during the early growth phase of normal cells cultivated in vitro (Russell & Snyder, 1968;Janne et al, 1978). Similarly, increases in the level of putrescine after the stimulation of ornithine decarboxylase have also been seen in cells either treated by carcinogens such as dimethylaminoazobenzene (Scalabrino et al, 1978) and tumour-promoting agents (O'Brien, 1976) or infected by oncogenic viruses (Don & Bachrach, 1975).…”

mentioning

confidence: 99%

Diamine oxidase activity in human melanoma cell lines with different tumorigenicity in nude mice

Thomasset¹,

Quash²,

Doré³

1982

Br J Cancer

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Summary.-The activity of diamine oxidase (DO, EC 1.4.3.6.) which converts putrescine into y-aminobutyraldehyde in the degradative pathway of polyamine, was studied in 4 human melanoma cell lines, 2 of which produce tumours in >80% of nude mice (M3Dau, M4Beu), whereas the other 2 induce tumours in <25% (M,Dor, M2GeB). The activity of DO in these cells varies with the growth rate: 24h after seeding there is an initial increase in DO activity, followed by a steep decline during exponential growth. At 96 h, when cells reach saturation density, the activity of DO is significantly greater in the highly tumorigenic cell lines than in the poorly tumorigenic cell lines. Kinetic studies show that for the highly tumorigenic lines apparent Km values are 10-6xlO-6M+0-2 (M3Dau) and 14'2 x 10-6M +0*6 (M4Beu), whereas for the poorly tumorigenic lines the values are 4.5X 10-6M +03. After transplantation into nude mice, the MjDor cell line, which exhibits a low Km (app.) for DO yielded tumour cells the DO of which had high Km (app.) value. Km (app.) determination of DO could be an approach for characterizing human melanoma cells differing in their tumorigenic potential in nude mice.

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Polyamines in rapid growth and cancer

Cited by 702 publications

References 221 publications

Effect of methionine deprivation on methylation and synthesis of macromolecules

Effect of methionine deprivation on methylation and synthesis of macromolecules

A Comparison of the Effects of Estradiol and 2- and 4-Hydroxyestradiol on Uterine Ornithine Decarboxylase Activity in Immature Rats

Diamine oxidase activity in human melanoma cell lines with different tumorigenicity in nude mice

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