Polyamines Involved in Regulating Self-Incompatibility in Apple

Yu, Jie; Wang, Baoan; Fan, Wenqi; Fan, Songbo; Xu, Ya; Liu, Chunsheng; Lv, Tianxing; Liu, Wanda; Wu, Ling; Xian, Linfeng; Li, Tianzhong

doi:10.3390/genes12111797

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…PAs in plants are involved in many processes, such as organogenesis, embryogenesis, floral and fruit development, leaf senescence, and plant abiotic and biotic stress responses. PAs are also highly critical in the process of plant reproduction, from pollen development to fertilization [3,4] to self-incompatibility [5]. In cells, their concentration depends on the balance among biosynthesis, degradation, and transport [6].…”

Section: Polyamines In Pollen Developmentmentioning

confidence: 99%

Male Fertility under Environmental Stress: Do Polyamines Act as Pollen Tube Growth Protectants?

Aloisi

Piccini

Cai

et al. 2022

IJMS

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Although pollen structure and morphology evolved toward the optimization of stability and fertilization efficiency, its performance is affected by harsh environmental conditions, e.g., heat, cold, drought, pollutants, and other stressors. These phenomena are expected to increase in the coming years in relation to predicted environmental scenarios, contributing to a rapid increase in the interest of the scientific community in understanding the molecular and physiological responses implemented by male gametophyte to accomplish reproduction. Here, after a brief introduction summarizing the main events underlying pollen physiology with a focus on polyamine involvement in its development and germination, we review the main effects that environmental stresses can cause on pollen. We report the most relevant evidence in the literature underlying morphological, cytoskeletal, metabolic and signaling alterations involved in stress perception and response, focusing on the final stage of pollen life, i.e., from when it hydrates, to pollen tube growth and sperm cell transport, with these being the most sensitive to environmental changes. Finally, we hypothesize the molecular mechanisms through which polyamines, well-known molecules involved in plant development, stress response and adaptation, can exert a protective action against environmental stresses in pollen by decoding the essential steps and the intersection between polyamines and pollen tube growth mechanisms.

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Section: Polyamines In Pollen Developmentmentioning

confidence: 99%

Male Fertility under Environmental Stress: Do Polyamines Act as Pollen Tube Growth Protectants?

Aloisi

Piccini

Cai

et al. 2022

IJMS

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“…Self-compatibility and SI have their own advantages and disadvantages for each individual crop and the aims of its cultivation. The ability to change SI to self-compatibility and vice versa is currently an important demand; different mechanisms, both physiological [109][110][111][112][113] and molecular-genetic [11,[113][114][115], are already used for this purpose.…”

Section: Ck As a Factor Of Si-induced Pcdmentioning

confidence: 99%

Hormonal Signaling during dPCD: Cytokinin as the Determinant of RNase-Based Self-Incompatibility in Solanaceae

Zakharova,

Khanina,

Knyazev

et al. 2023

Biomolecules

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Research into molecular mechanisms of self-incompatibility (SI) in plants can be observed in representatives of various families, including Solanaceae. Earlier studies of the mechanisms of S-RNase-based SI in petunia (Petunia hybrida E. Vilm.) demonstrate that programmed cell death (PCD) is an SI factor. These studies suggest that the phytohormon cytokinin (CK) is putative activator of caspase-like proteases (CLPs). In this work, data confirming this hypothesis were obtained in two model objects—petunia and tomato (six Solanaceae representatives). The exogenous zeatin treatment of tomato and petunia stigmas before a compatible pollination activates CLPs in the pollen tubes in vivo, as shown via the intravital imaging of CLP activities. CK at any concentration slows down the germination and growth of petunia and tomato male gametophytes both in vitro and in vivo; shifts the pH of the cytoplasm (PHc) to the acid region, thereby creating the optimal conditions for CLP to function and inhibiting the F-actin formation and/or destructing the cytoskeleton in pollen tubes to point foci during SI-induced PCD; and accumulates in style tissues during SI response. The activity of the ISOPENTENYLTRANSFERASE 5 (IPT5) gene at this moment exceeds its activity in a cross-compatible pollination, and the levels of expression of the CKX1 and CKX2 genes (CK OXIDASE/DEHYDROGENASE) are significantly lower in self-incompatible pollination. All this suggests that CK plays a decisive role in the mechanism underlying SI-induced PCD.

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“…Typically, seeds have three constituent parts: the seed coat, the embryo, and the endosperm [2]. Malus domestica is a common seed plant that does not normally produce fruit by means of self-pollination; only a few self-pollinating apple varieties have been discovered after rigorous screening processes [3][4][5][6][7][8][9][10][11]. 'Hanfu' is a cultivar that produces abundant fruit after self-pollination.…”

Section: Introductionmentioning

confidence: 99%

The Mechanism of Ovule Abortion in Self-Pollinated ‘Hanfu’ Apple Fruits and Related Gene Screening

Wei,

Wang,

et al. 2024

Plants

Self Cite

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Apples exhibit S-RNase-mediated self-incompatibility and typically require cross-pollination in nature. ‘Hanfu’ is a cultivar that produces abundant fruit after self-pollination, although it also shows a high rate of seed abortion afterwards, which greatly reduces fruit quality. In this study, we investigated the ovule development process and the mechanism of ovule abortion in apples after self-pollination. Using a DIC microscope and biomicroscope, we found that the abortion of apple ovules occurs before embryo formation and results from the failure of sperm–egg fusion. Further, we used laser-assisted microdissection (LAM) cutting and sperm and egg cell sequencing at different periods after pollination to obtain the genes related to ovule abortion. The top 40 differentially expressed genes (DEGs) were further verified, and the results were consistent with switching the mechanism at the 5′ end of the RNA transcript (SMART-seq). Through this study, we can preliminarily clarify the mechanism of ovule abortion in self-pollinated apple fruits and provide a gene reserve for further study and improvement of ‘Hanfu’ apple fruit quality.

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Polyamines Involved in Regulating Self-Incompatibility in Apple

Cited by 7 publications

References 32 publications

Male Fertility under Environmental Stress: Do Polyamines Act as Pollen Tube Growth Protectants?

Male Fertility under Environmental Stress: Do Polyamines Act as Pollen Tube Growth Protectants?

Hormonal Signaling during dPCD: Cytokinin as the Determinant of RNase-Based Self-Incompatibility in Solanaceae

The Mechanism of Ovule Abortion in Self-Pollinated ‘Hanfu’ Apple Fruits and Related Gene Screening

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