Polyamines reduces lipid peroxidation induced by different pro-oxidant agents

Bellé, N.A.V.; Dalmolin, Gerusa Duarte; Fonini, Graciela; Rubin, Maribel Antonello; Rocha, João Batista Teixeira da

doi:10.1016/j.brainres.2004.02.036

Cited by 244 publications

(134 citation statements)

References 54 publications

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“…In the experiment, Wister strain albino rats weighing 200-230 g were purchased from the breeding colony of Department of Veterinary Medicine, University of Ibadan, Nigeria. Rats were maintained at 25 °C, on a 12 h light/12 h dark cycle, with free access to food and water. They were acclimatized under these conditions for 2 weeks before the commencement of the experiment.…”

Section: Handling and Use Of Animalmentioning

confidence: 99%

“…Briefly, appropriate dilutions (0-200 毺L) of the extracts and 500 毺L of 0.02 M/L sodium phosphate buffer (pH 6.9 with 0.006 M/L sodium chloride) containing porcine pancreatic alpha-amylase (EC 3.2.1.1, 0.5 mg/mL) were incubated at 25 °C for 10 min. Then, 500 毺L of 10 g/L starch solution in 0.02 M/L sodium phosphate buffer (pH 6.9 with 0.006 M/L sodium chloride) was added to each tube.…”

Section: Alpha-amylase Inhibition Assaymentioning

confidence: 99%

“…Then, 500 毺L of 10 g/L starch solution in 0.02 M/L sodium phosphate buffer (pH 6.9 with 0.006 M/L sodium chloride) was added to each tube. The reaction mixtures was incubated at 25 °C for 10 min and stopped with 1.0 mL of dinitrosalicylic acid colour reagent. Thereafter, the mixture was incubated in a boiling water bath for 5 min and cooled to room temperature.…”

Section: Alpha-amylase Inhibition Assaymentioning

confidence: 99%

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In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe2+-induced lipid peroxidation in rat pancreas

Adefegha

Oboh

2012

Asian Pacific Journal of Tropical Biomedicine

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Section: Handling and Use Of Animalmentioning

confidence: 99%

Section: Alpha-amylase Inhibition Assaymentioning

confidence: 99%

Section: Alpha-amylase Inhibition Assaymentioning

confidence: 99%

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In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe2+-induced lipid peroxidation in rat pancreas

Adefegha

Oboh

2012

Asian Pacific Journal of Tropical Biomedicine

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“…This tissue was subsequently homogenized in cold saline (1/10 w/v) with about 10-up-and -down strokes at approximately 1,200 rev/min in a Teflon glass homogenizer. The homogenate was centrifuged for 10 min at 3000xg to yield a pellet that was discarded, and a low-speed supernatant (S1) was kept for lipid peroxidation assay (Belle et al 2004). …”

Section: Preparation Of Tissue Homogenatesmentioning

confidence: 99%

Characterization of the antioxidant properties of phenolic extracts from some citrus peels

2011

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This study sought to determine the distribution of free and bound phenolics in some Nigerian citrus peels [orange (Citrus sinensis), grapefruit (Citrus paradisii) and shaddock (Citrus maxima)] and characterize the antioxidant properties. The free phenolics were extracted with 80% acetone, while the bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Free phenolic extracts had significantly higher (P < 0.05) DPPH* scavenging ability than the bound phenolic extracts, except in orange peels where the bound phenolic extracts had significantly higher (P<0.05) DPPH* scavenging ability. Bound phenolics from orange peels had the highest ABTS* scavenging ability (6.09 mmol./TEAC g) and ferric reducing antioxidant properties (FRAP) (71.99 mg/GAE 100 g), while bound phenolics from shaddock peels had the least ABTS* scavenging ability (1.35 mmol./TEAC g) and FRAP (2.58 mg/GAE 100 g) . Bound phenolics from grapefruit peels had the highest OH* scavenging ability (EC 50 = 3.8 mg/ml), while bound phenolics from shaddock peels had the least (EC 50 =16.1 mg/ml). The phenolics chelated Fe 2+ and inhibited malondialdehyde production in rat's pancreas in a dose-dependent manner. The additive and/or synergistic action of the free and bound phenolics could have contributed to the observed medicinal properties of the peels; therefore, the high antioxidant properties of the free and bound phenolic extracts from orange peels could be harness in the formulation of nutraceuticals and food preservatives.

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“…These tissues were subsequently homogenized in cold saline (1:10, w/v) with about 10 up and down strokes at approximately 1200 rpm in a Teflon glass homogenizer. The homogenate was centrifuged for 10 min at 3000 × g to yield a pellet that was discarded, and a low -speed supernatant (SI) which was kept for the Lipid peroxidation assay (Belle, Dalmolin, Fonini, Rubin, & Rocha, 2004).…”

Section: Preparation Of Tissue Homogenatesmentioning

confidence: 99%

Bioactive components, antioxidative properties and inhibition of Fe2+-induced lipid peroxidation of mango peel as affected by the storage of mango fruit

Foluso

Makinde²,

Adeyemi³

et al. 2016

IJFS

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This study sought to evaluate the bioactive components (total phenolics, vitamin C and flavonoid), antioxidant properties (FRAP, and hydroxyl, DPPH and ABTS radical scavenging abilities) and inhibition of Fe 2+ -induced lipid peroxidation of the peel of mango fruit stored at refrigeration temperature and room temperature. The peel of mango fruit stored at room temperature had significantly (P ≤ 0.05) higher contents of total phenolic (13.61 mg GAE/g), vitamin C (12.98 mg AAE/g), total flavonoid (4.49 mg QE/g) and non-flavonoid (9.12 mg Qe/g) than the peel of freshly harvested mango fruit and the peel of mango fruit stored at refrigeration temperature. In consonance with the bioactive components, the peel of mango fruit stored at room temperature had a higher FRAP, and hydroxyl, DPPH and ABTS radical scavenging abilities than the others. The peel of mango fruit stored at room temperature showed stronger inhibition of Fe 2+ induced lipid peroxidation by exhibiting the least IC50 (1.44 mg/ml in brain), (1.43 mg/ml in pancreas) and (1.88 mg/ml in kidney). Thus freshly harvested, matured, edible and just ripe mango fruit (Sheri Mango) could be stored at room temperature and be consumed with the peel.

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Polyamines reduces lipid peroxidation induced by different pro-oxidant agents

Cited by 244 publications

References 54 publications

In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe2+-induced lipid peroxidation in rat pancreas

In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe2+-induced lipid peroxidation in rat pancreas

Characterization of the antioxidant properties of phenolic extracts from some citrus peels

Bioactive components, antioxidative properties and inhibition of Fe2+-induced lipid peroxidation of mango peel as affected by the storage of mango fruit

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