Polyanion–Tobramycin Nanocomplexes Into Functional Microparticles for the Treatment of Pseudomonas Aeruginosa Infections in Cystic Fibrosis

Abstract

MPs resulted to be a formulation of higher efficacy, with potential positive implications, as lower required dose, administration frequency, side effects and antibiotic resistance problems.

Structural Determinants of the Stability of Enzyme‐Responsive Polyion Complex Nanoparticles Targeting Pseudomonas aeruginosa’s Elastase

“…Polyion complex (PIC) (nano)particles, also known as polyelectrolyte complexes (PECs) or interpolyelectrolyte complexes (IPECs), are soft colloids obtained from the self‐assembly of oppositely charged polyelectrolytes in solution . These nanomaterials are attractive vehicles for the delivery of charged drugs such as antineoplastics, antimicrobials and nucleic acids, which can be complexed with oppositely charged polymers to form PIC (nano)particles that reduce the toxicity and/or control the activity of these drugs. Given the key role of enzymes in many diseases and their remarkable specificity, PIC (nano)particles prepared from polyelectrolytes that degrade in the presence of these enzymes have a great potential for biomedical applications.…”

Mucus and Cell-Penetrating Nanoparticles Embedded in Nano-into-Micro Formulations for Pulmonary Delivery of Ivacaftor in Patients with Cystic Fibrosis

“…CF-AM was prepared as previously described. ,,, Briefly, 500 mg of deoxyribonucleic acid (DNA), 250 mg of mucin, 0.295 mg of DTPA, 1 mL of RPMI 1640 amino acid solution, 250 μL of egg yolk emulsion, 250 mg of NaCl, and 110 mg of KCl were mixed together in a final volume of 50 mL of DNAse-free water. This dispersion was allowed to equilibrate at 25 °C for 2 h. Sterile CF-AM was obtained as follows: DNA and mucin were sterilized by exposure for 6 h to a germicidal lamp in a laminar flow hood.…”

“…Ivacaftor release assay in the presence of CF-AM was performed by using vertical Franz-type diffusion cells. , The system temperature was kept constant at 37 °C by recirculation of water from a thermostatically controlled bath. Continuous stirring at 180 rpm was provided by teflon-coated stirring bars placed in the acceptor chamber.…”

“…For this reason, two different copolymers were synthesized starting from α,β-poly­( N -2-hydroxyethyl)- d , l -aspartamide (PHEA), , a biocompatible water-soluble polyaminoacidic polymer, whose derivatives have been largely used for drug- and gene-delivery applications, − with a recent focus for pulmonary delivery. − …”

“…A nano - into - micro ( NiM ) strategy was then realized by spray-drying a dispersion of NPs in a solution containing mannitol, an osmotic agent already administered as an inhalation powder (Bronchitol, Pharmaxis Pharmaceuticals) with the ability to change the viscoelastic properties of the mucus, increase the hydration of the periciliary fluid layer, and contribute to increase the mucus clearance of the retained secretions through mucociliary activity…”