Polycationic lipids inhibit the pro-inflammatory response to LPS

Leon‐Ponte, Matilde; Kirchhof, Mark G.; Sun, Tina; Stephens, Tracey A.; Singh, Bhagirath; Sandhu, Shabaz; Madrenas, Joaquı́n

doi:10.1016/j.imlet.2004.07.019

Cited by 26 publications

(23 citation statements)

References 53 publications

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“…Recent reports suggest that SPs, particularly SP-A and SP-D, regulate a variety of immune cell functions in vitro, including enhanced chemotaxis and phagocytosis, and alterations in the production of reactive oxygen species and cytokines ( 56,57 ). However, work from our laboratories and those of others indicate that surfactant lipids, particularly the major surfactant lipid species DPPC, consistently apparent after 30 min of incubation with the lipids prior to cell stimulation with LPS (data not shown).…”

Section: Discussionmentioning

confidence: 64%

“…Hwang ( 65 ) suggested that saturated and unsaturated free fatty acids can differentially regulate COX-2 expression through effects on TLR4, although the mechanistic details were not given. A cationic lipid (lipofectamine) was shown to uncouple LPS binding and internalization from signaling by preventing CD14-TLR4 interactions ( 57 ). It has also been demonstrated recently that preincubation of endothelial cells with oxidized 1-palmitoyl-2-arachidonoyl-sn -glycero-3-phosphorylcholine can inhibit subsequent LPS-induced IL-8 synthesis by altering lipid raft/caveolar processing ( 66 ).…”

Section: Discussionmentioning

confidence: 99%

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Surfactant lipids regulate LPS-induced interleukin-8 production in A549 lung epithelial cells by inhibiting translocation of TLR4 into lipid raft domains

Abate

Alghaithy

Parton

et al. 2010

Journal of Lipid Research

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Surfactant lipids regulate LPS-induced interleukin-8 production in A549 lung epithelial cells by inhibiting translocation of TLR4 into lipid raft domains

Abate

Alghaithy

Parton

et al. 2010

Journal of Lipid Research

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“…Signaling of the LPS-CD14 complex through the TLR4 to activate the NF-kB and MAPK pathways. [29][30][31] In this study, we detected the down-regula- tion of Cdc91l1, Pigq, and Tlr4 in NS-treated BV-2 microglial cells (Table 1). The MAPK pathways are deeply involved in signaling for various immune responses including apoptosis.…”

Section: Fig 2 Dose-dependent Inhibitory Effects Of Nelumbinis Semementioning

confidence: 94%

The Genome-Wide Expression Profile of Nelumbinis semen on Lipopolysaccharide-Stimulated BV-2 Microglial Cells

Sohn

Chung

et al. 2009

Biological & Pharmaceutical Bulletin

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This study was conducted to evaluate the protective mechanisms of Nelumbinis semen (NS) on lipopolysaccharide (LPS)-induced activation of BV-2 microglial cells. The anti-inflammatory effects of NS were determined by analyzing nitric oxide production and proinflammatory cytokines using enzyme-linked immunosorbent assay. The mechanism was evaluated in BV-2 cells with or without NS treated with LPS for various lengths of time using oligonucleotide microarray and real time reverse transcription-polymerase chain reaction. The oligonucleotide microarray analysis revealed that mitogen activated protein kinase (MAPK) signaling pathway-related genes such as Fgfr3, Fgf12, Rasal2, Nfkb2, Map2k5, Mapk1, Map3k7, and NFatc2 were down-regulated in LPS activated BV-2 cells by pretreatment with NS. In addition, significant decreases in Nos1ap gene expression were observed with NS pretreatment. Cluster linked pathway analysis using the Kyoto Encyclopedia of Genes and Genomes database revealed that the effects of NS were closely associated with the regulation of mitochondria functions. These results suggested that NS can affect the MAPK signaling pathway and mitochondrial functions in BV-2 cells activated with LPS.

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“…Antimicrobial agents still represent the most important treatment to prevent sepsis, but they are insufficient, since there is currently no effective endotoxin-neutralizing treatment which would sequester LPS and prevent excessive response by the innate immune system. Lipopolyamines have recently shown promising results for neutralization of endotoxin (2,(5)(6)(7)14). Lipopolyamines are polycationic amphiphilic molecules originally used as DNA transfection agents.…”

mentioning

confidence: 99%

Combination of Antimicrobial and Endotoxin-Neutralizing Activities of Novel Oleoylamines

Zorko

Majerle

Šarlah

et al. 2005

Antimicrob Agents Chemother

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A combination of antimicrobial and endotoxin-neutralizing activities is desired in order to prevent progression from infection to sepsis due to the release of lipopolysaccharide from dying gram-negative bacteria. Lipopolyamines have emerged as a new type of endotoxin-neutralizing compound, but their antimicrobial activity has not been investigated. We synthesized a series of 10 oleoylamines differing in the polyamino head group, particularly in the number and separation between nitrogen atoms and the position of the oleoyl moiety. Compounds showed activity against both gram-negative and gram-positive bacteria in the micromolar range. Compounds were able to provide penetration of ethidium bromide into bacteria, indicating effects on the bacterial membrane. Oleoylamines neutralized endotoxin in Limulus amoebocyte lysate assays and by neutralization of tumor necrosis factor alpha release in human blood. Comparison of biological activities of compounds identified structural properties responsible for antimicrobial activity, and quantitative structureproperty relationship analysis provided a quantitative model for prediction of activity of oleoylamines.

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Polycationic lipids inhibit the pro-inflammatory response to LPS

Cited by 26 publications

References 53 publications

Surfactant lipids regulate LPS-induced interleukin-8 production in A549 lung epithelial cells by inhibiting translocation of TLR4 into lipid raft domains

Surfactant lipids regulate LPS-induced interleukin-8 production in A549 lung epithelial cells by inhibiting translocation of TLR4 into lipid raft domains

The Genome-Wide Expression Profile of Nelumbinis semen on Lipopolysaccharide-Stimulated BV-2 Microglial Cells

Combination of Antimicrobial and Endotoxin-Neutralizing Activities of Novel Oleoylamines

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