Water reclamation and ecological reuse is gradually becoming a popular solution to address the high pollutant loads and insufficient ecological flow of many urban rivers. However, emerging contaminants in water reuse system and associated human health and ecological risks need to be assessed. This study determined the occurrence and human health and ecological risk assessments of 35 emerging contaminants during one year, including 5 types of persistent organic pollutants (POPs), 5 pharmaceutical and personal care products (PPCPs), 7 endocrine disrupting chemicals (EDCs) and 18 disinfection by-products (DBPs), in a wastewater treatment plant (WWTP) and receiving rivers, as well as an unimpacted river for comparison. Results showed that most of PPCPs and EDCs, especially antibiotics, triclosan, estrogens and bisphenol A, occurred frequently at relatively high concentrations, and they were removed from 20.5% to 88.7% with a mean of 58.9% via WWTP. The highest potential noncarcinogenic and carcinogenic risks in different reuse scenarios were assessed using maximal detected concentrations, all below the acceptable risk limits, with the highest total combined risk value of 9.21 × 10 −9 and 9.98 × 10 −7 , respectively. Ecological risk assessment was conducted using risk quotient (RQ) method and indicated that several PPCPs, EDCs and haloacetonitriles (HANs) pose high risk (RQ N 1) to aquatic ecology in the rivers, with the highest RQ up to 83.8. The study suggested that ecological risks need to be urgently addressed by updating and optimizing the process in WWTPs to strengthen the removal efficiencies of emerging contaminants.