Polyclonal antibodies to EB1089 (seocalcitol), an analog of 1α,25-dihydroxyvitamin D3☆

Blaehr, Lars K. A.; Björkling, Fredrik; Binderup, Ernst; Calverley, Martin J.; Kaastrup, Peter

doi:10.1016/s0039-128x(00)00225-7

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…They were air-dried and incubated with PBS/1%BSA (Sigma) for 20 min. Then the cells were stained with the different antibodies (antiannexin IImAb, Transduction Laboratories; and anti-1[alpha],25-dihydroxyvitamin D 3 , provided by Leo Pharma) [Blaehr et al, 2001[Blaehr et al, , 2003] for 1 h. After three washes in PBS, cells were stained with Cy3 and Cy2 (Jackson Immuno Research) for 1 h, washed in PBS and embedded in Vectashield. The microscopy was done with a Leica CLSM version TCS NT.…”

Section: Immunofluorescencementioning

confidence: 99%

1[alpha],25‐dihydroxyvitamin D₃enhances annexin II dependent proliferation of osteoblasts

Maier

Scherer

Seifert

et al. 2006

J of Cellular Biochemistry

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Cells experience a variety of physiological and non-physiological stresses and consequently have appropriate mechanisms to deal with such deviations from homeostasis. Particularly subject to mechanical stress and shear forces are the cells that make up the bones. Osteoblastic cells can interpret this stress as a stimulus for proliferation; however, the molecular mechanisms underlying this phenomenon are poorly understood. We have identified annexin II as being specifically upregulated in mechanically stressed osteoblasts and found that increased levels of this protein are necessary for 1[alpha],25-dihydroxyvitamin D(3) mediated augmentation of the proliferative response of osteoblasts after mechanical stress. Our data demonstrate a novel interaction between 1[alpha],25-dihydroxyvitamin D(3) and annexin II in the proliferative response of osteoblasts as well as a novel function for annexin II in the stress response. These findings may offer new therapeutic opportunities for conditions that require regenerative osteoblastic activity such as osteoporosis.

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Section: Immunofluorescencementioning

confidence: 99%

1[alpha],25‐dihydroxyvitamin D₃enhances annexin II dependent proliferation of osteoblasts

Maier

Scherer

Seifert

et al. 2006

J of Cellular Biochemistry

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Analysis of Vitamins D, Their Metabolites and Analogues

et al. 2010

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Polyclonal antibodies to EB1089 (seocalcitol), an analog of 1α,25-dihydroxyvitamin D3☆

Cited by 2 publications

References 14 publications

1[alpha],25‐dihydroxyvitamin D₃enhances annexin II dependent proliferation of osteoblasts

1[alpha],25‐dihydroxyvitamin D₃enhances annexin II dependent proliferation of osteoblasts

Analysis of Vitamins D, Their Metabolites and Analogues

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Polyclonal antibodies to EB1089 (seocalcitol), an analog of 1α,25-dihydroxyvitamin D3☆

Cited by 2 publications

References 14 publications

1[alpha],25‐dihydroxyvitamin D3enhances annexin II dependent proliferation of osteoblasts

1[alpha],25‐dihydroxyvitamin D3enhances annexin II dependent proliferation of osteoblasts

Analysis of Vitamins D, Their Metabolites and Analogues

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Product

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1[alpha],25‐dihydroxyvitamin D₃enhances annexin II dependent proliferation of osteoblasts

1[alpha],25‐dihydroxyvitamin D₃enhances annexin II dependent proliferation of osteoblasts