Polyclonal antibody against Manduca sexta chitinase and detection of chitinase expressed in transgenic cotton

Hao, Chan-juan; Chai, Baofeng; Wang, Wei; Sun, Yi; Liang, Aihua

doi:10.1007/s10529-004-6935-0

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…Hao et al, 2005;McCafferty et al, 2006). Purified chitinases fed to honeybees and bumblebees have had no effect on a range of measured characteristics, and chitinaseexpressing GM plants have not affected honeybee foraging behaviour (Table 9.2).…”

Section: Transgenic Plants Expressing Other Insecticidal Proteinsmentioning

confidence: 99%

Environmental Impact of Genetically Modified Crops

Ferry¹,

Gatehouse²

2009

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This book, containing 20 chapters, addresses the major concerns of scientists, policy makers, environmental lobby groups and the general public regarding the controversial issue on environmental impact (e.g. on soil and water ecology and nontarget organisms) of transgenic crops, from an editorially neutral standpoint. While the main focus is on environmental impact, food safety issues for both humans and animals are also considered. Some chapters discuss issues on resistance management. The book concludes with a discussion on the future of agricultural biotechnology in the context of sustainability, natural resource management and future global population and food supply.

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Section: Transgenic Plants Expressing Other Insecticidal Proteinsmentioning

confidence: 99%

Environmental Impact of Genetically Modified Crops

Ferry¹,

Gatehouse²

2009

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“…In a former study, we had established an efficient method to prepare the specific polyclonal antibody against Manduca Sexta chitinase (Hao et al 2005). A lot of toxin was needed in this step.…”

Section: Confirmation Of the Titer Of Polyclonal Antibodymentioning

confidence: 99%

Polyclonal Antibody Against a Recombinant Chlorotoxin-like Peptide from the Chinese Scorpion and Detection of its Putative Receptors in Human Glioma Cells

Yin

Liang

2006

Biotechnol Lett

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The nucleotide sequence of a type of chlorotoxin-like peptide, an inhibitor of small-conductance Cl(-) channels, from the scorpion, Buthus martensii Karsch, was synthesized (named rBmK CTa) according to the sequence optimized for codon usage in E. coli. It was over-expressed using a pExSecI expression system and purified to homogeneity. Polycolonal antibodies to the purified protein were raised in rats. Overlay assay and pull-down assay showed that this toxin specially binds to two proteins in the glioma cells with corresponding molecular weights of about 80 and 35 kDa. They may serve as candidate receptors or alternative cellular component for interaction with rBmK CTa.

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“…Chitinase in baculovirus and Beauveria bassiana was found involved in the pathogenesis against insects (Fan et al ., 2007). Chitinase genes were cloned into transgenic cotton (Hao et al ., 2005) and Bacillus thuringiensis (Arora et al ., 2003; Lertcanawanichakul et al ., 2004; Hu et al ., 2009) for pest control. On the other hand, Chitinase-mediated chitin degradation plays an important role in the molting and pupation of insects (Schrempf, 2001; Carlini & Grossi-De-Sá 2002; Saville et al ., 2002).…”

Section: Introductionmentioning

confidence: 99%

Functional expression of theSpodoptera exiguachitinase to examine the virtually screened inhibitor candidates

Zhang

Guan

Pan

et al. 2019

Bull. Entomol. Res.

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Chitinase is responsible for insect chitin hydrolyzation, which is a key process in insect molting and pupation. However, little is known about the chitinase of Spodoptera exigua (SeChi). In this study, based on the SeChi gene (ADI24346) identified in our laboratory, we constructed the recombinant baculovirus P-Chi for the expression of recombinant SeChi (rSeChi) in Hi5 cells. The rSeChi was purified by chelate affinity chromatography, and the purified protein showed activity comparable with that of a commercial SgChi, suggesting that we harvested active SeChi for the first time. The purified protein was subsequently tested for enzymatic properties and revealed to exhibit its highest activity at pH 8 and 40 C. Using homology modeling and molecular docking techniques, the three-dimensional model of SeChi was constructed and screened for inhibitors. In two rounds of screening, twenty compounds were selected. With the purified rSeChi, we tested each of the twenty compounds for inhibitor activity against rSeChi, and seven compounds showed obvious activity. This study provided new information for the chitinase of beet armyworm and for chitinase inhibitor development.

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Polyclonal antibody against Manduca sexta chitinase and detection of chitinase expressed in transgenic cotton

Cited by 6 publications

References 7 publications

Environmental Impact of Genetically Modified Crops

Environmental Impact of Genetically Modified Crops

Polyclonal Antibody Against a Recombinant Chlorotoxin-like Peptide from the Chinese Scorpion and Detection of its Putative Receptors in Human Glioma Cells

Functional expression of theSpodoptera exiguachitinase to examine the virtually screened inhibitor candidates

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