Polyclonal B cell activation in paracoccidioidomycosis

Chequer-Bou-Habib, Dumith; Daniel-Ribeiro, Cláudio Tadeu; Banic, Dalma Maria; Valle, Antonio C. Franciscone do; Galvão–Castro, Bernardo

doi:10.1007/bf00436058

Cited by 28 publications

(18 citation statements)

References 15 publications

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“…Sometimes, the concentration of IgG parallels the activities of complement-fixing or -precipitating antibodies detected by gel diffusion (79,214,278). A study to determine the nature of the precipitating antibodies concluded that they are of the IgG class (108 Circulating immune complexes in paracoccidioidomycosis patients were first described by Arango et al (9) and later confirmed by Chequer-Bou-Habib et al (68,69 6,1993 to prepare, long shelf life, specific (no cross-reactions), reproducible, sensitive, reliable, and reactive in more than one serological test. Different antigen preparations vary greatly in quality, depending on fungal strain, morphological phase, culture medium, size of inoculum, incubation time, and recovery techniques (242,290).…”

Section: Microbiologymentioning

confidence: 99%

“…Usually, humoral immunity is preserved, and the patient exhibits polyclonal activation of B cells (68). Cell-mediated immune functions are depressed in the severe, multifocal form of disease but preserved in less serious cases.…”

Section: Infectionmentioning

confidence: 99%

“…6,1993 Antigenemia. The description of immune complexes in patients with paracoccidioidomycosis (9,68,69) and the observation of precipitin lines occurring between serum samples set up in the immunodiffusion and counterimmunoelectrophoresis systems suggested for the first time that there was circulating antigen in patient sera (280). Subsequently, attempts have been made to diagnose this mycosis by detecting circulating antigens in serum samples.…”

Section: Microbiologymentioning

confidence: 99%

“…A strong argument in support of this hypothesis is that impairment of cell-mediated responses is reversible by successful therapy (197,264). The search for a factor(s) responsible for negative immunoregulation of cellular immunity during paracoccidioidomycosis has ranged from unidentified inhibitory factors in patient plasma (208,264) and immune complexes (9,68,69,304) to an imbalance in T-cell subsets (200,204).…”

Section: Cell-mediated Responsesmentioning

confidence: 99%

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Paracoccidioidomycosis: an update

1993

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This review summarizes knowledge on various aspects of paracoccidioidomycosis. Mycelial propagules, chlamydospores, and arthroconidia exhibit thermal dimorphism; arthroconidia are infectious in animals and, by electron microscopy, appear well provided for survival. The mycelial-to-yeast-phase transformation requires a strict control of glucan synthesis probably mediated by membrane enzymes. Hormonal influences on the transformation of the fungus (mycelium or conidium to yeast phase) have been demonstrated. Estrogen-binding proteins have been detected in the fungal cytosol, and during the transformation novel proteins are produced as a result of estradiol incorporation. Clinical forms have been better defined on the basis of better experimental models. Emphasis has been placed on the lungs as the portal of entry and on the existence of silent pulmonary infections. A specific Paracoccidioides brasiliensis antigen, the 43-kDa glycoprotein (Gp43), has been identified, characterized, and cloned. This has led to improved reproducibility and specificity of serologic tests. The depression of cell-mediated immune responses has been associated with severe disease in humans and in the experimental host. T-cell subsets in patients' tissues were characterized by means of monoclonal antibodies, and a reduced CD4/CD8 ratio was demonstrated. This has been related to alterations in lymphokine and tumor necrosis factor production, production of antigen-antibody complexes, etc. Amphotericin B has provided effective therapy. Azole derivatives have also improved prognosis and facilitated therapy. Itraconazole is presently the drug of choice, yet incapacitating sequelae (mainly pulmonary fibrosis) still constitute major problems.

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Section: Microbiologymentioning

confidence: 99%

Section: Infectionmentioning

confidence: 99%

Section: Microbiologymentioning

confidence: 99%

Section: Cell-mediated Responsesmentioning

confidence: 99%

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Paracoccidioidomycosis: an update

1993

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“…10,11 Paracoccidioidomycosis is characterized by a polyclonal activation of B cells resulting in high levels of serum IgG. 12,13 The detection of antigen-specific IgE in diseases associated with polyclonal B-cell activation and hypergammaglobulinemia is difficult because of IgG antibody competition for the same epitopes.…”

Section: Introductionmentioning

confidence: 99%

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

Mamoni¹,

Rossi²,

Camargo³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved.

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New insights into a complex fungal pathogen: the case of Paracoccidioides spp.

González

Hernández

2016

Yeast

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Paracoccidioidomycosis is a systemic mycosis endemic to Latin America, with Paracoccidioides brasiliensis and P. lutzii being the causal agents of this disorder. Several issues have been raised in the 100 years since its discovery and in this article we discuss features of this fascinating fungal pathogen, including its biology, eco-epidemiology and aspects of its pathogenicity. We also consider some of its virulence determinants, the most recent advances in the study of its metabolic pathways and the molecular and genetic research tools developed for this research. We also review the animal models used to study host-fungal interactions and how the host defence mechanisms against this pathogen work.

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Polyclonal B cell activation in paracoccidioidomycosis

Cited by 28 publications

References 15 publications

Paracoccidioidomycosis: an update

Paracoccidioidomycosis: an update

Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis.

New insights into a complex fungal pathogen: the case of Paracoccidioides spp.

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