Polyclonal Ig production after Epstein-Barr virus infection of human lymphocytes in vitro

Production of Human Monoclonal Antibody to X31 Influenza Virus Nucleoprotein

“…This virus is a human herpesvirus which only infects B lymphocytes (Pattengale et al, 1973), transforming them into lymphoblastoid cell lines with the capacity for indefinite growth in culture (Pope et al, 1968). These transformed cells synthesize and secrete immunoglobulin (Rosen et al, 1977), and recently this fact has been exploited to produce cell lines which secrete specific antibodies (Steinitz et al, 1977(Steinitz et al, , 1980Kozbor et al, 1979;Kozbor & Roder, 1981). Cloning of these cell lines has led to the production in vitro of human monoclonal antibodies (Kozbor & Roder, 1981 ;Steinitz & Tamir, 1982).…”

“…No staining was detected with antibodies to human IgM, IgD (Naiem et al, 1981), IgG2, IgG3, IgG4, or 2 light chain. (These antibodies were kindly provided by Dr N. Ling, Birmingham, U.K. ; Dr D. Mason, Oxford, U.K. and Dr M. Cooper, Birmingham, Ala., U.S.A.) Very few IgG class human monoclonal antibodies have been derived from EB virustransformed cell lines, a fact which probably reflects the apparent preference of EB virus to infect IgM-bearing B cells (Rosen et al, 1977). The quantity of specific anti-X31 antibody in the supernatant medium from cloned C10 cultures which had been left undisturbed for 5 days was measured by the ELISA technique using a standard serum of known antibody titre (Zanders et al, 1981).…”

Bone marrow-derived lymphoid cell lines from patients with agammaglobulinemia.

“…In vitro stimulation of peripheral lymphocytes by mitogens, including pokeweed mitogen (PWM), lymphocyte mitogenic factor (LMF), or EBV, results in the increased synthesis and eventual secretion of IgM or IgG by B lymphocytes (11)(12)(13)(14)(15). Morphologically, the small B lymphocytes enlarge and undergo mitosis, and differentiate into immunoglobulin-secreting lymphoblasts.…”

A novel IgM class autoantibody to a hepatocyte-related 190 kDa molecule in patients with type 1 autoimmune hepatitis

“…To prepare human MoAb, we used peripheral blood mononuclear cells (PBMC) infected with Epstein-Barr virus (EBV) in vitro. 15,16 In brief, we cultured PBMC from a patient with the classical features of type 1 AIH 17 ; this EBV-producing cell line was designated SB91. Supernatants from these EBV-transformed cells were selected by FACS (see Flow Cytometry Analysis).…”