Polycyclic aromatic hydrocarbon (PAH) source identification and a maternal transfer case study in threatened killer whales (Orcinus orca) of British Columbia, Canada

Abstract

The northeastern Pacific (NEP) Ocean spans the coast of British Columbia (Canada) and is impacted by anthropogenic activities including oil pipeline developments, maritime fossil fuel tanker traffic, industrial chemical effluents, agricultural and urban emissions in tandem with stormwater and wastewater discharges, and forest wildfires. Such events may expose surrounding marine environments to toxic polycyclic aromatic hydrocarbons (PAHs) and impact critical habitats of threatened killer whales (Orcinus orca). We analyzed skeletal muscle and liver samples from stranded Bigg’s killer whales and endangered Southern Resident killer whales (SRKWs) for PAH contamination using LRMS. C3-phenanthrenes/anthracenes (mean: 632 ng/g lw), C4-dibenzothiophenes (mean: 334 ng/g lw), and C4-phenanthrenes/anthracenes (mean: 248 ng/g lw) presented the highest concentrations across all tissue samples. Diagnostic ratios indicated petrogenic-sourced contamination for SRKWs and pyrogenic-sourced burdens for Bigg’s killer whales; differences between ecotypes may be attributed to habitat range, prey selection, and metabolism. A mother-fetus skeletal muscle pair provided evidence of PAH maternal transfer; low molecular weight compounds C3-fluorenes, dibenzothiophene, and naphthalene showed efficient and preferential exposure to the fetus. This indicates in-utero exposure of PAH-contamination to the fetus. Our results show that hydrocarbon-related anthropogenic activities are negatively impacting these top predators; preliminary data found here can be used to improve oil spill and other PAH pollution management and regulation efforts, and inform policy to conserve killer whale habitats in the NEP.

Polycyclic Aromatic Hydrocarbons (PAHs) in Freshwater Systems: A Comprehensive Review of Sources, Distribution, and Impacts

“…Liver and muscle tissues from stranded individuals were analyzed. The findings revealed significant PAH concentrations, particularly C3-phenanthrenes/anthracenes, C4-dibenzothiophenes, and C4phenanthrenes/anthracenes, indicating substantial contamination across the sampled tissues [237]. The analysis also differentiated the sources of PAHs affecting each killer whale group; SRKWs showed signs of petrogenic (oil-derived) contamination, while Bigg's killer whales had pyrogenic (fire-related) burdens.…”

“…The analysis also differentiated the sources of PAHs affecting each killer whale group; SRKWs showed signs of petrogenic (oil-derived) contamination, while Bigg's killer whales had pyrogenic (fire-related) burdens. This variation likely stems from differences in their living areas, diet, and metabolic processes [237]. A particularly concerning discovery was the evidence of PAH transfer from mother to fetus, observed through a mother-fetus muscle sample pair.…”

“…In exploring the impact of PAHs in specific water systems, several case studies offer valuable insights. A study by Lee et al analyzed PAH contamination in whales, revealing evidence of maternal transfer of PAHs to the fetus, indicating in-utero exposure to PAH contamination [237]. Liver and muscle tissues from stranded individuals were analyzed.…”

“…A particularly concerning discovery was the evidence of PAH transfer from mother to fetus, observed through a mother-fetus muscle sample pair. It was found that low molecular weight PAHs such as C3-fluorenes, dibenzothiophene, and naphthalene were efficiently transferred to the fetus, showcasing in-utero exposure to these toxic substances [237].…”