Polydiacetylene Liposome Microarray Toward Influenza A Virus Detection: Effect of Target Size on Turn‐On Signaling

Abstract

Target size effect on the sensory signaling intensity of polydiacetylene (PDA) liposome microarrays was systematically investigated. Influenza A virus M1 peptide and M1 antibody were selected as a probe-target pair. While red fluorescence from the PDA liposome microarrays was observed when the larger M1 antibody was used as a target, when the same M1 antibody was used as a probe to detect the smaller M1 peptide sensory signal did not appear. The results reveal that the intensity of the PDA sensory signal is mainly related to the steric repulsion between probe-target complexes not the strength of the probe-target binding force. Based on this finding, we devised a PDA sensory system that directly detects influenza A whole virus as a larger target, and confirmed the target size effect on the signaling efficiency of PDA.

Effect of ethanol on the color transition of the polydiacetylene vesicle of 10,12‐pentacosadiynoic acid for butylamine detection

“…Polydiacetylenes (PDAs) are polymers in which double bonds and triple bonds are alternately conjugated, and they generally have a blue color. Because their color can be changed by outer stimuli, a lot of studies have been conducted in which PDAs have been used as sensing materials . A color change means that detection is possible with the naked eye and without any detection instruments.…”

Colorimetric detection of influenza A (H1N1) virus by a peptide-functionalized polydiacetylene (PEP-PDA) nanosensor

“…18,19 Among the many colorimetric detection approaches, polydiacetylene (PDA) has been extensively studied as a colorimetric sensing material, owing to its unique chromatic properties. [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] The self-assembly of diacetylene monomers into spherical PDA vesicles with a conjugated backbone of alternating double and triple bonds (ene-yene chain) occurs upon ultraviolet (UV) irradiation. Unperturbed PDA vesicles typically exhibit an intense blue color because of electronic absorption via a p-p* transition of the electrons in the conjugated backbone.…”

Colorimetric Detection of Some Highly Hydrophobic Flavonoids Using Polydiacetylene Liposomes Containing Pentacosa-10,12-diynoyl Succinoglycan Monomers

“…Schematic illustration of a self-assembly by PCDA and SGM1-PCDA were shown as Fig 10A and 10B . Recent study has reported that the intensity of PDA color transition could be due to a steric repulsion rather than the strength of the binding force [ 40 ]. The conformational change of PDA liposome through steric repulsion induced by intermolecular complexes produces the blue to red color transition [ 41 , 42 ].…”