Polydimethyl siloxane microfluidic channel protein functionalization techniques

Khnouf, Ruba; Karasneh, Dina

doi:10.1109/nems.2016.7758279

Cited by 1 publication

(2 citation statements)

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“…Recently we reported the synthesis of a triblock copolymer (TBC) consisting of a conjugated polydiketo-pyrrolopyrrole-thienothiophene (PDPP-TT) PSC flanked by two soft elastic segments, i.e., PDMS (Figure A) . PDMS moieties offer not only stretchability but also the possibility to perform reliable physical adsorption of biochemical species, e.g., antibodies or proteins . The utilization of such a block copolymer might therefore serve as a strong foundation to realize a straightforward, low-cost, and wearable biosensing platform.…”

Section: Introductionmentioning

confidence: 99%

“…22 PDMS moieties offer not only stretchability but also the possibility to perform reliable physical adsorption of biochemical species, e.g., antibodies or proteins. 30 The utilization of such a block copolymer might therefore serve as a strong foundation to realize a straightforward, low-cost, and wearable biosensing platform. It has been over a year since the COVID-19 outbreak was declared a pandemic by the World Health Organization (WHO).…”

Section: Introductionmentioning

confidence: 99%

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Rapid Detection of SARS-CoV-2 Antigens and Antibodies Using OFET Biosensors Based on a Soft and Stretchable Semiconducting Polymer

Ditte

Ditzer

et al. 2021

ACS Biomater. Sci. Eng.

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In the midst of the COVID-19 pandemic, adaptive solutions are needed to allow us to make fast decisions and take effective sanitation measures, e.g., the fast screening of large groups (employees, passengers, pupils, etc.). Although being reliable, most of the existing SARS-CoV-2 detection methods cannot be integrated into garments to be used on demand. Here, we report an organic field-effect transistor (OFET)-based biosensing device detecting of both SARS-CoV-2 antigens and anti-SARS-CoV-2 antibodies in less than 20 min. The biosensor was produced by functionalizing an intrinsically stretchable and semiconducting triblock copolymer (TBC) film either with the anti-S1 protein antibodies (S1 Abs) or receptor-binding domain (RBD) of the S1 protein, targeting CoV-2-specific RBDs and anti-S1 Abs, respectively. The obtained sensing platform is easy to realize due to the straightforward fabrication of the TBC film and the utilization of the reliable physical adsorption technique for the molecular immobilization. The device demonstrates a high sensitivity of about 19%/dec and a limit of detection (LOD) of 0.36 fg/mL for anti-SARS-Cov-2 antibodies and, at the same time, a sensitivity of 32%/dec and a LOD of 76.61 pg/mL for the virus antigen detection. The TBC used as active layer is soft, has a low modulus of 24 MPa, and can be stretched up to 90% with no crack formation of the film. The TBC is compatible with roll-to-roll printing, potentially enabling the fabrication of low-cost wearable or on-skin diagnostic platforms aiming at point-of-care concepts.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%