Polydopamine–Gelatin as Universal Cell-Interactive Coating for Methacrylate-Based Medical Device Packaging Materials: When Surface Chemistry Overrules Substrate Bulk Properties

Abstract

Despite its widespread application in the fields of ophthalmology, orthopedics, and dentistry and the stringent need for polymer packagings that induce in vivo tissue integration, the full potential of poly(methyl methacrylate) (PMMA) and its derivatives as medical device packaging material has not been explored yet. We therefore elaborated on the development of a universal coating for methacrylate-based materials that ideally should reveal cell-interactivity irrespective of the polymer substrate bulk properties. Within this perspective, the present work reports on the UV-induced synthesis of PMMA and its more flexible poly(ethylene glycol) (PEG)-based derivative (PMMAPEG) and its subsequent surface decoration using polydopamine (PDA) as well as PDA combined with gelatin B (Gel B). Successful application of both layers was confirmed by multiple surface characterization techniques. The cell interactivity of the materials was studied by performing live-dead assays and immunostainings of the cytoskeletal components of fibroblasts. It can be concluded that only the combination of PDA and Gel B yields materials possessing similar cell interactivities, irrespective of the physicochemical properties of the underlying substrate. The proposed coating outperforms both the PDA functionalized and the pristine polymer surfaces. A universal cell-interactive coating for methacrylate-based medical device packaging materials has thus been realized.

Endothelialization and Anticoagulation Potential of Surface‐Modified PET Intended for Vascular Applications

“…Unmodified PET presented a hydrophobic surface with an WCA of ≈73°, while a noticeable increase in hydrophilicity was recorded after each surface modification step (i.e., after AFB*SE and/or gelatin binding). This was an important observation when considering materials for medical applications, as it is known that hydrophilic surfaces in general and gelatin‐modified surfaces, in particular, exhibit enhanced biocompatibility …”

3D-printed titanium implant-coated polydopamine for repairing femoral condyle defects in rabbits

“…The ideal goal of implant surface modification could achieve strong osseointegration and improve long-term stability and the osseointegration depends on direct interaction between the bone tissue and biomaterial surface. The 3D PPT coating is one of the most common methods for surface modification of biomaterials and can improve the process of osseointegration [11][12][13][14][15][16].…”

“…The preparation of the PDA coating was performed according to the described method [10,11] (Figs. 1 and 2).…”

Deregulation of focal adhesion formation and cytoskeletal tension due to loss of A-type lamins

“…Image processing was performed in Fiji (http://fiji.sc), a packaged version of ImageJ freeware. 67 The focal adhesion points were quantified by means of a customdesigned image processing pipeline, 68 which is essentially based on a high-content analysis workflow described before 20 and is available upon request. In brief, the analysis consists of a few image preprocessing steps, followed by hierarchical segmentation of nuclei, cells, and focal adhesion points (FAs) to allow region-specific analysis of objects.…”