Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V

Abstract

Infections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) were attached to the surface of a titanium alloy, Ti6Al4V, by conjugation to a polydopamine linking substrate. The topography of the polydopamine coating was evaluated by electron microscopy and coating thickness measurements were performed with ellipsometry and Atomic Force Microscopy (AFM). The subsequently attached peptide stability was investigated with release profile studies in simulated body fluid, using both fluorescence imaging and High-Performance Liquid Chromatography (HPLC). Finally, the hydrophobicity of the coating was characterised by water contact angle measurements. The designed AMPs were shown to provide long-term bonding to the polydopamine-coated Ti6Al4V surfaces.

Application of Antimicrobial Peptides on Biomedical Implants: Three Ways to Pursue Peptide Coatings

“…Lastly, human osteosarcoma cells were cultivated on the metal plates and beside the ones modified with KR-12/32-5911, the cells attached densely on all other surfaces, they were viable and able to proliferate. Based on their results, the authors claimed the potential of the herein presented antimicrobial metallic surfaces that were modified with KR12, KR12/32, or KR12-5911, respectively, for application as biomedical materials [35]. However, additional strategies to circumvent the observed toxicity of AMPs have to be developed.…”

Antimicrobial peptides for bone tissue engineering: Diversity, effects and applications

“…Specifically, because the quinine and catechol groups of Frontiers in Bioengineering and Biotechnology frontiersin.org polydopamine can create a chelated structure with the material through a series of chemical reactions, it has broad adhesion properties and can adhere to almost any surface. The mechanism by which polydopamine coatings can be used to load bioactive factors such as AMPs lies in the fact that such amine-containing nucleophilic reagents can be coupled to pDAs via Schiff base reactions or Michael addition reactions of benzoquinone groups under weak alkaline conditions, leading to secondary modification (Trzcińska et al, 2020). The advantages of the polydopamine coating strategy are low cost, ease of handling, wide application and optimized biocompatibility.…”

“…The advantages of the polydopamine coating strategy are low cost, ease of handling, wide application and optimized biocompatibility. So this strategy is also widely used in AMPs delivery system ( He S et al, 2018 ; Meng et al, 2020 ; Trzcińska et al, 2020 ). For example, Trzcińska et al (2020) .…”

“…So this strategy is also widely used in AMPs delivery system ( He S et al, 2018 ; Meng et al, 2020 ; Trzcińska et al, 2020 ). For example, Trzcińska et al (2020) . Introduced an autonomously synthesized KR-12 analogue on the surface of titanium implants via a polydopamine coating.…”

Bioinspired Polydopamine Coatings Facilitate Attachment of Antimicrobial Peptidomimetics with Broad-Spectrum Antibacterial Activity

“…This polymerised layer has numerous catechol groups allowing the covalent attachment of thiol or amine groups [ 35 ]. This simple and versatile coating method has been used to attach antimicrobial peptides to a variety of biomaterial surfaces, including titanium, glass, plastics, polymers, gauze and stainless steel [ 36 , 37 , 38 ]. Covalently attaching peptidomimetics using dopamine-functionalised surfaces has significant potential for these coatings to be applied clinically to medical devices.…”