Polydopamine Nanosheets Doped Injectable Hydrogel with Nitric Oxide Release and Photothermal Effects for Bacterial Ablation and Wound Healing

Abstract

The development of wound dressings with combined antibacterial activities and pro-healing functions has always been an intractable medical task for treating bacterial wound infection. Herein, a novel injectable hybrid hydrogel dressing is developed, which is doped with nitric oxide (NO) donor (N,N'-di-sec-butyl-N,N'-dinitroso-1,4-phenylenediamine, BNN6) loaded two-dimensional polydopamine nanosheets (PDA NS). The hydrogel matrix is in situ formed through dynamic Schiff base crosslinking between hydrazide-modified 𝜸-polyglutamic acid (𝜸-PGA-ADH) and aldehyde-terminated Pluronic F127 (F127-CHO). Under 808 nm irradiation, the embedded PDA NS exhibits outstanding photothermal transform properties (56.1%) and on-demand NO release. The combination of photothermal and NO gas therapy with a synergistic antibacterial effect works on both Escherichia coli and Staphylococcus aureus in vitro. Furthermore, a full-thickness skin defect model also demonstrates that the hybrid hydrogel shows outstanding antibacterial properties and effectively accelerates the wound healing process. Overall, this study provides a facile and promising method for the fabrication of PDA NS based multifunctional hydrogel dressing for the application of infectious skin wound healing.

Polydopamine: a bioinspired adhesive and surface modification platform

“…More recent reviews have highlighted the role of PDA in rechargeable batteries, 26 catalysis 27 and especially in biomedicine 28,29 . These include a focus on nanostructures and interfaces, 15,30 as well as the development of PDA antibacterial surfaces 31 …”

“…A titanium alloy was coated with PDA to improve stability and then collagen was conjugated onto the surface with the hope of facilitating soft tissue adhesion 44 . Liu and coworkers have used PDA nanosheets to fabricate multifunctional injectable hydrogel wound dressing 15 . The nanosheets were prepared by the autooxidation of dopamine in the presence of DNA.…”

“…More recent reviews have highlighted the role of PDA in rechargeable batteries, 26 catalysis 27 and especially in biomedicine. 28,29 These include a focus on nanostructures and interfaces, 15,30 as well as the development of PDA antibacterial surfaces. 31 PDA in batteries can be used to enhance the thermal stability of existing materials and can be used as the actual electrode material.…”

“…For sensing applications, the abundance of functional groups on PDA allows for species such as biotin, fluorescent labels, DNA and proteins to be linked to particle or film surfaces. 15 Some of the earlier examples in this field include PDA coatings for biosensing and in biofuel cells. 20 Building on earlier reports, Yang et al have recently developed a 'turn-on' glucose sensor by preparing fluorescent nanodots of PDA and treating them with AgNO 3 .…”

Controllable Nitric Oxide‐Delivering Platforms for Biomedical Applications

“…N,N-bis(Carboxymethyl)-N,N-dinitroso-p-phenylenediamine (BNN6) is the most commonly used N-nitrosoamine. [158,159] Based on the second approach, Huang et al utilized magnesium silicate-coated upconverting nanoparticles UCNPs@MgSiO 3 based on a yolk-shell structure as a carrier to load the NO precursor BNN6 and anticancer drug DOX Figure 22. [160] UCNPs can convert NIR to UV or visible light, activating the loaded BNN6 to release NO, which avoids the phototoxicity of direct UV light excitation.…”