Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease

Hermanto, Dhony; Mudasir, Mudasir; Siswanta, Dwi; Kuswandi, Bambang; Ismillayli, Nurul

doi:10.5614/j.math.fund.sci.2019.51.3.8

Cited by 21 publications

(5 citation statements)

References 20 publications

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“…The crosslinked beads were filtered and stored at 4 °C for future use. The beads were lyophilized and surface morphology was observed using scanning electron microscopy on a SEM, Quanta FEG 250 machine [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

et al. 2022

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In the current study, the purified β-mannanase (Man/Cel5B) from Thermotoga maritima was immobilized on glutaraldehyde cross-linked chitosan beads. The immobilization of Man/Cel5B on chitosan beads was confirmed by Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. After immobilization, the protein loading efficiency and immobilization yield were found to be 73.3% and 71.8%, respectively. The optimum pH for both free and immobilized enzymes was found to be pH 5.5. However, the optimum temperature of immobilized Man/Cel5B increased by 10 °C, from 85 °C (free Man/Cel5B) to 95 °C (Immobilized). The half-life of free and immobilized enzymes was found to be 7 h and 9 h, respectively, at 85 °C owing to the higher thermostability of immobilized Man/Cel5B. The increase in thermostability was also demonstrated by an increase in the energy of deactivation (209 kJmol−1) for immobilized enzyme compared to its native form (92 kJmol−1), at 85 °C. Furthermore, the immobilized Man/Cel5B displayed good operational stability as it retained 54% of its original activity after 15 repeated catalytic reactions concerning its free form.

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Section: Methodsmentioning

confidence: 99%

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

et al. 2022

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“…ALG as a polyanion and CS as a polycation can create interactions between ALG carboxyl groups and CS amino groups. The combination of these polymers is characterized by better mechanical and thermal stability and higher adhesive properties than their constituent polymers [ 6 , 14 , 15 ].…”

Section: Resultsmentioning

confidence: 99%

Does the Freeze–Thaw Technique Affect the Properties of the Alginate/Chitosan Glutamate Gels with Posaconazole as a Model Antifungal Drug?

Szekalska

Sosnowska

Wróblewska

et al. 2022

IJMS

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Hydrogels are semi-solid systems with high flexibility, which, due to holding large amounts of water, are similar to natural tissues and are very useful in the field of biomedical applications. Despite the wide range of polymers available to form hydrogels, novel techniques utilized to obtain hydrogels with adequate properties are still being developed. The aim of this study was to evaluate the impact of the freeze–thaw technique on the properties of cryogels based on sodium alginate and chitosan glutamate with posaconazole as a model antifungal substance. The effect of the freezing and thawing process on the physicochemical, rheological, textural and bioadhesive properties of prepared cryogels was examined. Additionally, the antifungal activity against Candida albicans, Candida parapsilosis and Candida krusei of designed formulations was examined. It was shown that the freeze–thaw technique significantly improved viscosity, bioadhesiveness, textural properties and prolonged the in vitro posaconazole release. Moreover, alginate/chitosan glutamate cryogels exhibited higher values of inhibition zone in C. parapsilosis culture than traditional hydrogel formulations.

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“…Here, the morphology of the alginatechitosan membrane mixed was less homogeneous, it can be observed that the transparent membrane surface of the alginate-chitosan shows a fibrous structure with a rough morphology and irregular shape, which is a network of electrostatic interactions of alginate and chitosan. The likeness of its surface morphology has been described at previously work 6,9 .…”

Section: Preparation Of Transparent Pec Membranementioning

confidence: 99%

“…The membrane was prepared from hydrosols of chitosan and alginate, as described by Hermanto et al 6,9 . The scanning electron microscope (SEM) study was carried out on a transparent PEC membrane after being coated with gold palladium on SEM (Joel LV 5600 USA model) to evaluate the morphology and structure of membrane surface, as shown in Fig.…”

Section: Preparation Of Transparent Pec Membranementioning

confidence: 99%

“…The formed PEC is expected to provide better applications due to its unique structure and properties 8 . Recently, it has been developed a sensor using PEC alginate-chitosan matrix as immobilized membrane of bioactive material 9 . The membrane is suitable for immobilizing active material to produce a selective sensing interface for detection of target analytes 10 .…”

Section: Introductionmentioning

confidence: 99%

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Transparent Membrane of Polyelectrolyte Complex Doped with 2,6-Dichlorophenol-Indophenol as an Optical Sensor for Colorimetric Measurements of Ascorbic acid

Hermanto¹,

Ismillayli²,

Honiar³

et al. 2020

Orient. J. Chem

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An optical chemical sensor based on a transparent membrane of polyelectrolyte complex (PEC), composed of alginate-chitosan membrane and 2,6-dichlorophenol-indophenol (2,6-DCPIP), coated on transparent mica was coupled with UV-Vis spectrophotometry and used for colorimetric measurements of ascorbic acid (AA). The transparent membranes of PEC were characterized using scanning electron microscopy (SEM) technique. The experimental parameters of the biosensor were optimized. Here, the optical chemical sensor showed maximum response at wavelength of 545 nm, with the optimum pH at 3. The calibration curve had a dynamic working range at 0.0 to 5.0 mM of AA with a limit of detection (LOD) of 0.13 mM AA. The membrane sensor has a long life time in the defined condition (4 o C). The developed optical chemical sensor correlated well with the standard method, HPLC for the determination of Ascorbic acid concentration in real samples.

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Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease

Cited by 21 publications

References 20 publications

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads

Does the Freeze–Thaw Technique Affect the Properties of the Alginate/Chitosan Glutamate Gels with Posaconazole as a Model Antifungal Drug?

Transparent Membrane of Polyelectrolyte Complex Doped with 2,6-Dichlorophenol-Indophenol as an Optical Sensor for Colorimetric Measurements of Ascorbic acid

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