Polyelectrostatic immobilization of gold nanoparticles-modified peroxidase on alginate-coated gold electrode for mediatorless biosensor construction

Chico, Belkis; Camacho, Conrado; Pérez, M Rosales; Longo, María A.; Sanromán, M.A.; Pingarrón, José M.; Villalonga, Reynaldo

doi:10.1016/j.jelechem.2009.02.004

Cited by 30 publications

(17 citation statements)

References 28 publications

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“…In addition, several ionic polysaccharides such as sodium alginate (Camacho et al, 2007, Ionescu et al, 2006, Cosnier et al, 2004 and chitosan and its derivatives (Qin et al, 2006, Li et al, 2008, have been successfully used as coating materials for preparing robust enzyme biosensors. Horseradish peroxidase was cross-linked with cysteamine-capped Au nanoparticles and further immobilized on sodium alginate-coated Au electrode through polyelectrostatic interactions (Chico et al, 2009). The electrode was employed for constructing a reagentless amperometric biosensor for H 2 O 2 .…”

Section: Horseradish Peroxidasementioning

confidence: 99%

Nanobiosensors for health care

Atta¹,

Galal²,

Ali³

2011

Biosensors for Health, Environment and Biosecurity

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Section: Horseradish Peroxidasementioning

confidence: 99%

Nanobiosensors for health care

Atta¹,

Galal²,

Ali³

2011

Biosensors for Health, Environment and Biosecurity

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“…Horseradish peroxidase was modified with cysteamine-capped Au nanospheres and further immobilized on sodium alginate-coated Au electrode through polyelectrostatic interactions for developing mediatorless H2O2 quantifying biosensor (14). Moreover, the gold nanosphere-mesoporous silica composite represented excellent catalytic behavior to the oxidation of glucose, faster response time, lower detection limit, higher sensitivity and wider linear range with respect to solely use gold nanosphere.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

“…Especially, amino groups are introduced on the surface of particles, followed by modified with glutaraldehyde bearing a free aldehyde group. Those modified particles were reacted, via Schiff's base formation reaction, with the amino groups on the enzyme surface (14).…”

Section: Covalent Bondingmentioning

confidence: 99%

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Inorganic nanomaterial-based biocatalysts

Lee

Lee²,

Chang³

et al. 2011

BMB Reports

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Over the years, nanostructures have been developed to enable to support enzyme usability to obtain highly selective and efficient biocatalysts for catalyzing processes under various conditions. This review summarizes recent developments in the nanostructures for enzyme supporters, typically those formed with various inorganic materials. To improve enzyme attachment, the surface of nanomaterials is properly modified to express specific functional groups. Various materials and nanostructures can be applied to improve both enzyme activity and stability. The merits of the incorporation of enzymes in inorganic nanomaterials and unprecedented opportunities for enhanced enzyme properties are discussed.

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“…This previous study also revealed that the uptake of CeO 2 NPs by corn plants was affected by soil organic matter content and alginate surface coating (Zhao et al, 2012a). Alginates are naturally occurring polysaccharides (Chen and Elimelech, 2008;Kantar et al, 2008) that have been used to stabilize NPs for several applications (Li et al, 2008;Chico et al, 2009;Fayaz et al, 2009;dos Santos Silva et al, 2011). This suggests that excess of alginate can be released into the environment together with NPs, with unknown consequences for edible plants.…”

Section: Introductionmentioning

confidence: 92%

Alginate modifies the physiological impact of CeO2 nanoparticles in corn seedlings cultivated in soil

Zhao

Peralta-Videa

Peng

et al. 2014

Journal of Environmental Sciences

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a b s t r a c tAlginates are naturally occurring components of organic matter in natural soil whose effects on nanoparticles (NPs) toxicity to plants is not well understood. In the present study, corn plants were grown for one month in soil spiked with 400 mg/kg CeO 2 NPs with various alginate concentrations. After one month of growth in the NPs impacted soil, plants were harvested and analyzed for Ce and mineral element concentrations. Chlorophyll concentration and heat shock protein 70, used as biomarkers for oxidative stress, were also evaluated. Results showed that, compared to CeO 2 NPs treatment, alginate at 10, 50, and 100 mg/kg increased Ce concentration in roots by approximately 46%, 38%, and 29% and by 115%, 45%, and 56% in shoots, respectively. CeO 2 NPs without alginate increased Mn accumulation in roots by 34% compared to control. CeO 2 NPs with low and medium alginate increased Mn by ca. 92% respect to NPs without alginate and by ca. 155% respect to control. CeO 2 NPs without/with alginate significantly increased accumulation of Fe and Al in roots. In addition, alginate at 50 mg/kg increased Zn accumulation in roots by 52% compared to control. In shoots, K increased at all NP treatments but the accumulation of other elements was not affected. Alginate enlarged the impact of CeO 2 NPs to corn plants by reducing chlorophyll a content and triggering overexpression of heat shock protein 70.

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Polyelectrostatic immobilization of gold nanoparticles-modified peroxidase on alginate-coated gold electrode for mediatorless biosensor construction

Cited by 30 publications

References 28 publications

Nanobiosensors for health care

Nanobiosensors for health care

Inorganic nanomaterial-based biocatalysts

Alginate modifies the physiological impact of CeO2 nanoparticles in corn seedlings cultivated in soil

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