Polyester fabric modification by chemical treatment to enhancing the β-glucosidase immobilization

Almulaiky, Yaaser Q.

doi:10.1016/j.heliyon.2022.e11660

Cited by 13 publications

(3 citation statements)

References 48 publications

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“…45,46 An increase in K m value was also observed by Almulaiky and co-workers for ⊎-glucosidase immobilized on polyester fabric and chitosan metal-organic frameworks. 47,48 These results were in line with previously reported work. 49,50 Furthermore, V max decreased, with the free enzyme exhibiting a V max value of 18.08 μmol L −1 min −1 compared with 13.42 μmol L −1 min −1 for the immobilized enzyme (supporting information, Fig.…”

Section: Enzyme Kinetics and Reusability Studysupporting

confidence: 92%

Magnetically separable spent coffee grounds as a potential novel support for the covalent immobilization of β‐glucosidase for cellobiose hydrolysis

Bhardwaj,

Mishra,

Ghosh

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUNDThe industrial‐scale application of enzymes faces obstacles due to elevated costs and difficulties in stability and reuse. In this study, magnetic spent coffee grounds, an ecotoxic waste, have been utilized successfully for the first time to immobilize β‐glucosidase to overcome these challenges.RESULTSThe spent coffee grounds were magnetized and amine‐functionalized, followed by characterization using various techniques. Under optimized conditions, forming an imine bond between the functionalized support and β‐glucosidase resulted in a 62% immobilization yield (92.81 mg g−1 enzyme loading) and 12.5 U mg−1 activity after immobilization. A relatively small kinetic change was observed in the Km value (902 to 946 μmol L−1) after immobilization, suggesting minimal hindrance by AMSCG3 on substrate access or product release. Moreover, Glu@AMSCG3 showed exceptional stability (>90% residual activity) within a pH range of 3 to 6 after 2 h of incubation at 25 °C. A residual activity of 87.94% was maintained even at 80 °C and pH 5 after 2 h of incubation compared to the free β‐glucosidase, which showed only 6.5% residual activity at the same temperature. When cellobiose was hydrolyzed using Glu@AMSCG3 under optimum conditions, 91.33% cellobiose conversion was achieved initially, and over 79% conversion was maintained for 10 reusability cycles.CONCLUSIONThe improved stability of β‐glucosidase after covalent immobilization on amine‐modified magnetically separable spent coffee grounds indicates their potential as a support matrix for application in enzyme immobilization. © 2024 Society of Chemical Industry (SCI).

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Section: Enzyme Kinetics and Reusability Studysupporting

confidence: 92%

Magnetically separable spent coffee grounds as a potential novel support for the covalent immobilization of β‐glucosidase for cellobiose hydrolysis

Bhardwaj,

Mishra,

Ghosh

et al. 2024

J of Chemical Tech & Biotech

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“…Modifications made on the PET products by aminolysis for biomedical and industrial applications are very promising but mostly they are in the embryonic stages. [108][109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][124]128 In some cases, when used in the human body, it takes years to find the results of in vivo applications, although, already considerable progress has been made. By choosing proper amine and reaction condition time and temperature, the negative effects of amines can become negligence.…”

Section: Discussionmentioning

confidence: 99%

“…Modifications made on the PET products by aminolysis for biomedical and industrial applications are very promising but mostly they are in the embryonic stages 108–124,128 . In some cases, when used in the human body, it takes years to find the results of in vivo applications, although, already considerable progress has been made.…”

Section: Discussionmentioning

confidence: 99%

Modification of poly(ethylene terephthalate) fibers, fabrics, and films by amination reactions—A review

Rabiei,

Haghighat Kish

2024

Polymers for Advanced Techs

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Currently, many of our living facilities are dependent on poly(ethylene terephthalate) (PET). It is utilized in the production of textiles, beverage bottles, food packaging, implants, and so forth. Although PET has desirable properties, it has to be amended for certain applications that have been modified by different methods. For modifications, aminolysis is used by several investigators, and this report reviews critically the related literature. Amines can be applied to impart the desired properties and produce active sites to be used for further alterations by successive reactions. Amine treatments alone enhance wettability, increase dyeability, and most applicable, produce functionality on the surface. By further reactions with several other chemicals, a spectrum of properties was endowed to PET products, such as flame retarding, anti‐fouling property, highest hygroscopicity, silk‐like character, biocompatibility, and so forth. There are a large number of works, mainly to change the surface properties, but the literature on the in vivo or common everyday evaluation of the amended products is scarce. Most of the modifications of PET products by amines are accompanied by a decrease in some other properties. Then the process of aminolysis and modifications have to be optimized with respect to several production parameters.

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Endoxylanase Immobilized Nanoporous Silica for the Production of Xylooligosaccharides: Equilibrium Kinetics, Thermodynamic Studies, and Enzyme Characteristics

Kartik,

Dutta,

Shivudu

et al. 2024

ChemistrySelect

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Nanoporous structured silica materials, namely f‐SiO2, SBA‐15, IITM‐41, and MCM‐41 were employed as the matrices for immobilizing endoxylanase by adsorption method. The equilibrium kinetics, activation energy, and thermodynamic parameters associated with endoxylanase adsorption were investigated. Our findings revealed a two‐phase adsorption mechanism: an initial phase featuring rapid adsorption rates, succeeded by a slower phase wherein adsorption gradually progressed until equilibrium was attained. Analysis of the adsorption kinetic data indicated a better fit with the pseudo‐second‐order model, suggesting chemisorption and highlighting its temperature dependency. The calculated activation energy (Ea) values fell within the range of physisorption, indicating the involvement of both types of adsorption processes. Thermodynamic assessments confirmed that the adsorption reactions were spontaneous, feasible, and endothermic. Notably, the immobilization process did not change the optimum pH of the enzyme, while the optimum temperature shifted slightly. Furthermore, immobilized enzymes show a higher reaction rate (Vmax) than the soluble XynC. All immobilized xylanases produced xylobiose (X2) to xylohexose (X6) by hydrolyzing the xylan substrate. Recycling studies showed that up to 80 % of the yield was retained after seven cycles of reuse. Our study demonstrates the potential of nanostructured silica as an effective immobilization matrix for enzymes of industrial significance.

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Polyester fabric modification by chemical treatment to enhancing the β-glucosidase immobilization

Cited by 13 publications

References 48 publications

Magnetically separable spent coffee grounds as a potential novel support for the covalent immobilization of β‐glucosidase for cellobiose hydrolysis

Magnetically separable spent coffee grounds as a potential novel support for the covalent immobilization of β‐glucosidase for cellobiose hydrolysis

Modification of poly(ethylene terephthalate) fibers, fabrics, and films by amination reactions—A review

Endoxylanase Immobilized Nanoporous Silica for the Production of Xylooligosaccharides: Equilibrium Kinetics, Thermodynamic Studies, and Enzyme Characteristics

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