Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT

Lalitkumar, Sujata; Boggavarapu, Nageswara Rao; Menezes, Judith; Dimitriadis, Evdokia; Zhang, Jianguo; Nicola, Nicos A.; Gemzell‐Danielsson, Kristina; Lalitkumar, Luther

doi:10.1016/j.fertnstert.2013.06.023

Cited by 23 publications

(19 citation statements)

References 188 publications

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“…mosaic and aneuploid). Euploid blastocysts displayed negligible levels of apoptosis in the TE as well as in the ICM ( Figure 2B), agreeing with previous observations made in normal human blastocysts (17,18).…”

Section: Fands26828r1 Style Revision ! 19supporting

confidence: 92%

One hundred mosaic embryos transferred prospectively in a single clinic: exploring when and why they result in healthy pregnancies

Victor

Tyndall²,

Brake³

et al. 2019

Fertility and Sterility

164

151

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Section: Fands26828r1 Style Revision ! 19supporting

confidence: 92%

One hundred mosaic embryos transferred prospectively in a single clinic: exploring when and why they result in healthy pregnancies

Victor

Tyndall²,

Brake³

et al. 2019

Fertility and Sterility

164

151

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“…Recently, vaginal administration of PEGLA in mice has been proven to be effective in inhibiting embryo implantation [103] while systemic administration of this compound to cynomolgus monkey reduced endometrial STAT3 phosphorylation, inhibited LIF-induced expression of cochlin, insulin-like growth factor-binding protein- (IGF-BP-) 3, vascular endothelial growth factor- (VEGF-) A, and COX-2 enzyme which are essential for embryo implantation [104]. While most works related to the use of LIF antagonist and PEGLA as nonhormonal contraceptive agents were preliminary and were limited to animal studies, a study using human tissue has been recently performed in vitro by Lalitkumar et al [105]. In this study, the effect on human embryo attachment rate, embryo quality, and blastocyst expression of cell survival factor (Akt) and caspase-3 following exposure to endometrial tissue collected at luteal day 4 (LH+4) treated with PEGLA were determined.…”

Section: Lif As a Target For Nonhormonal Contraceptionmentioning

confidence: 99%

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

Salleh

Giribabu

2014

The Scientific World Journal

110

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Leukaemia inhibitory factor (LIF) plays an indispensible role in embryo implantation. Aberrant LIF production is linked to implantation failure. LIF regulates multiple processes prior to and during implantation such as uterine transformation into a receptive state, decidualization, blastocyst growth and development, embryo-endometrial interaction, trophoblast invasion, and immune modulation. Due to its critical role, LIF has been a target for a nonhormonal contraception. In this review, we summarize up-to-date information on the role of LIF in implantation and its role in contraception.

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“…This is a relevant concern as pharmacokinetic studies have shown rapid absorption and clearance of recombinant LIF after subcutaneous injection (70). A recent experiment utilized in vitro human endometrial constructs containing stroma and glandular epithelium obtained from uterine biopsies to measure human embryo attachment rate (71). Following exposure to a LIF antagonist, attachment of the embryo to the construct and subsequent hatching were both decreased.…”

Section: Steroid-induced Epithelial-stromal Crosstalk Involving Glandmentioning

confidence: 99%

Minireview: Steroid-Regulated Paracrine Mechanisms Controlling Implantation

Pawar

Hantak

Bagchi

et al. 2014

Molecular Endocrinology

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Implantation is an essential process during establishment of pregnancy in mammals. It is initiated with the attachment of the blastocyst to a receptive uterine epithelium followed by its invasion into the stromal tissue. These events are profoundly regulated by the steroid hormones 17β-estradiol and progesterone. During the past several years, mouse models harboring conditional gene knockout mutations have become powerful tools for determining the functional roles of cellular factors involved in various aspects of implantation biology. Studies using these genetic models as well as primary cultures of human endometrial cells have established that the estrogen receptor α, the progesterone receptor, and their downstream target genes critically regulate uterine growth and differentiation, which in turn control embryo-endometrial interactions during early pregnancy. These studies have uncovered a diverse array of molecular cues, which are produced under the influence of estrogen receptor α and progesterone receptor and exchanged between the epithelial and stromal compartments of the uterus during the progressive phases of implantation. These paracrine signals are critical for acquisition of uterine receptivity and functional interactions with the embryo. This review highlights recent work describing paracrine mechanisms that govern steroid-regulated uterine epithelial-stromal dialogue during implantation and their roles in fertility and disease.

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Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT

Cited by 23 publications

References 188 publications

One hundred mosaic embryos transferred prospectively in a single clinic: exploring when and why they result in healthy pregnancies

One hundred mosaic embryos transferred prospectively in a single clinic: exploring when and why they result in healthy pregnancies

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

Minireview: Steroid-Regulated Paracrine Mechanisms Controlling Implantation

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