Polygonatum sibiricum polysaccharide alleviates inflammatory cytokines and promotes glucose uptake in high‑glucose‑ and high‑insulin‑induced 3T3‑L1 adipocytes by promoting Nrf2 expression

Abstract

Polygonatum sibiricum polysaccharide (PSP) has been shown to alleviate hyperglycemia and reduce oxidative stress to delay the progression of diabetic retinopathy and cataracts. However, its role and underlying mechanisms in regulating type 2 diabetes mellitus (T2dM) remain unclear. nuclear factor erythroid 2-related factor 2 (nrf2) activation plays a protective role in T2dM. The present study focused on the effect of PSP on inflammatory cytokine secretion and Nrf2 expression in the adipocytes of T2dM patients. in this study, high-glucose-and high-insulin-induced 3T3-l1 adipocytes were used to mimic insulin-resistant (ir)-3T3-l1 adipocytes. Furthermore, the effect and underlying mechanisms of PSP on inflammation and glucose uptake in IR-3T3-L1 adipocytes were investigated. The present study found that proliferation after 50, 100 and 250 µg/ml PSP treatment had no significant change in normal 3T3-l1 adipocytes. a total of 50, 100 and 250 µg/ml of PSP also alleviated il-1β, il-6, and TnF-α levels and promoted proliferation, glucose uptake, and glucose transporter 4 expression in ir-3T3-l1 adipocytes. Furthermore, 50, 100 and 250 µg/ml PSP promoted nrf2 and Ho-1 expression. However, silencing nrf2 expression reversed the effect of 100 µg/ml PSP in ir-3T3-l1 adipocytes. in conclusion, these results suggest that PSP alleviates inflammatory cytokines and promotes glucose uptake in IR-3T3-L1 adipocytes by promoting nrf2 expression. PSP may be a potential therapeutic agent for T2dM treatment by promoting nrf2 expression.

Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns of IL6 and ADIPOQ Genes and Their Associated Epigenetic Modifications in Differentiating Human Visceral Adipocytes

“…In the research of Youssef-Elabd et al, neither chronic nor transient HG treatment of subcutaneous Ads altered the secretion of IL-6 [27]. By contrast, Cai et al showed elevated release of IL-6 from the 3T3-L1 Ads exposed to HG [28]. Our results did not fully support the notion that HG enhances IL-6 production in Ads.…”

Mechanochemical prepared ibuprofen-Polygonatum sibiricum polysaccharide drug delivery system for enhanced bioactivity with reduced renal injury induced by NSAIDs

“…Polygonatum sibiricum is a traditional Chinese medicine for renal protection and treating nephropathy (Bibi et al., 2016 ; Cai et al., 2019 ; Zhao et al., 2019 ; Han et al., 2020 ; Wang et al., 2020 ). It has been widely used as a functional food around all Asian countries.…”

“…Cai et al. ( 2019 ) found that PSP could reduce inflammatory cytokines and promote glucose uptake by promoting the expression of Nrf2. Li et al.…”

Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD‑induced mouse obesity via regulation of lipid metabolism and inflammatory response

“…P. sibiricum polysaccharide (PSP) is mainly composed of galactose, mannose, glucose and galacturonic acid, in molar ratios of 29.63:36.10:15.09:10.20 ( 22 ). PSP has been verified to exert a variety of medicinal functions, including anti-inflammatory ( 23 ) and anti-tumor effects ( 24 ), lowering blood glucose ( 25 ), enhancing immunity and promoting the differentiation of bone cells ( 20 ).…”