Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis

Neoh, Soon Zher; Chek, Min Fey; Tan, Hua; Linares-Pastén, Javier A.; Nandakumar, Ardra; Hakoshima, Toshio; Sudesh, Kumar

doi:10.1016/j.crbiot.2022.01.002

Cited by 40 publications

(20 citation statements)

References 63 publications

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“…68 For readers' interest, Neoh et al have, in a recent comprehensive review, elucidated the role of phaC as a key enzyme in microbial biopolymer synthesis. 67 Overall, the utilization of coenzyme A, a thioester of either short or medium chain length (r)-hydroxy fatty acids, leads to two distinct forms of PHA depending on the class of phaC employed as earlier explained. For instance, short-chain-length PHAs (scl, C3−C5) are derived from hydroxy fatty acids produced by bacteria such as C. necator and Alcaligenes latus.…”

Section: ■ Introduction Of Bioplasticsmentioning

confidence: 93%

“…The PHA synthase enzyme, phaC, is important in the polymerization of microbial biopolymers, especially polyesters containing 2-hydroxy acid constituents . Therefore, these phaCs are crucial enzymes in PHA production and are categorized into Class I, Class II, Class III, and Class IV depending on protein sequence and substrate specificities, among other factors . Classes I, III, and IV preferentially use hydroxy fatty acids produced by bacteria, such as C. necator , Allochromatium vinosum , and Bacillus megaterium , respectively, to produce scl-PHAs.…”

Section: Poly(hydroxyalkanoates (Pha) Origin Types Properties and Bio...mentioning

confidence: 99%

“…It is worth noting that phaC genes expressed in E. coli (recombinant E. coli ) are used to catalyze the polymerization of mcl-hydroxy acyl-CoA monomers to PHA copolymers such as poly(3HB- co -3HHx) which exhibit superior properties compared to scl-PHAs or mcl-PHAs . For readers’ interest, Neoh et al have, in a recent comprehensive review, elucidated the role of phaC as a key enzyme in microbial biopolymer synthesis …”

Section: Poly(hydroxyalkanoates (Pha) Origin Types Properties and Bio...mentioning

confidence: 99%

“…66 Therefore, these phaCs are crucial enzymes in PHA production and are categorized into Class I, Class II, Class III, and Class IV depending on protein sequence and substrate specificities, among other factors. 67 Classes I, III, and IV preferentially use hydroxy fatty acids produced by bacteria, such as C. necator, Allochromatium vinosum, and Bacillus megaterium, respectively, to produce scl-PHAs. On the other hand, class II phaCs from bacteria such as Pseudomonas aeruginosa are more inclined to mcl-PHAs catalysis through the β-oxidation or malonyl-CoA pathways.…”

Section: ■ Introduction Of Bioplasticsmentioning

confidence: 99%

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Poly(hydroxyalkanoates): Production, Applications and End-of-Life Strategies–Life Cycle Assessment Nexus

Muiruri

Yeo

Zhu

et al. 2022

ACS Sustainable Chem. Eng.

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The runaway production and consumption of oilbased plastics are key drivers of global warming and the increased carbon footprint. Besides, most of this plastic debris ends up in the oceans and constitutes about 80% of all marine debris. This pollution problem calls for a seismic shift to eco-friendly plastics and marine biodegradable ones. Unlike other biobased polymers, polyhydroxyalkanoates (PHAs) take pride in their degradation in soil and marine environments. This intriguing marine biodegradation property of PHAs sets it apart as the best choice to curb microplastics, particularly in marine ecosystems. PHAs have also grown in popularity due to other quintessential properties such as biocompatibility, structural variety, and similarity to conventional plastics in terms of physical properties. PHAs are being widely researched for various applications, including packaging, medical, energy, and agriculture. This perspective comprehensively focuses on the state-of-art production and applications of PHA plastics as well as the practical recycling strategies for postconsumer PHAs. The innovative "next generation industrial biotechnology" (NGIB) is well covered in this perspective. Moreover, the nexus between end-of-life strategies and life cycle assessment (LCA) of PHAs waste is elucidated to understand its impact on the environment thoroughly.

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Section: ■ Introduction Of Bioplasticsmentioning

confidence: 93%

Section: Poly(hydroxyalkanoates (Pha) Origin Types Properties and Bio...mentioning

confidence: 99%

Section: Poly(hydroxyalkanoates (Pha) Origin Types Properties and Bio...mentioning

confidence: 99%

Section: ■ Introduction Of Bioplasticsmentioning

confidence: 99%

See 2 more Smart Citations

Poly(hydroxyalkanoates): Production, Applications and End-of-Life Strategies–Life Cycle Assessment Nexus

Muiruri

Yeo

Zhu

et al. 2022

ACS Sustainable Chem. Eng.

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“…There are four classes of PHA synthase, each with unique characteristics 43 . Class I and II PHA synthases are composed solely of PhaC.…”

Section: Pha-associated Gene Clustermentioning

confidence: 99%

Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’

Jeon

Jiang

Peng

et al. 2023

Preprint

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A rod-shaped, motile, Gram-negative bacterial strain named DM-R-R2A-13T was isolated from the plant Cannabis sativa L. ‘Cheungsam’. The phylogenetic analysis of the 16S rRNA gene sequence revealed that strain DM-R-R2A-13T belongs to the family Oxalobacteraceae and is closely related to members of the genus Massilia, with Massilia flava (97.58% sequence similarity) and Massilia armeniaca (97.37% sequence similarity) being the closest members. The digital DNA-DNA hybridization (dDDH) values between strain DM-R-R2A-13T and Massilia flava CGMCC 1.10685T and Massilia armeniaca ZMN-3Twere 22.2% and 23.3%, while the average nucleotide identity (ANI) values were 78.85% and 79.63%, respectively. The DNA G + C content was measured to be 64.6 mol%. Moreover, the bacterium was found to contain polyhydroxyalkanoate (PHA) granules based on transmission electron microscopy, indicating its potential to produce bioplastic. Genome annotation revealed the presence of PHA synthase genes (phaC, phaR, phaP, and phaZ), and the biopolymer was identified as poly-3-hydroxybutyrate (PHB) based on nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) analyses. Using maltose as a carbon source, the strain produced PHB of up to 13.5% of its dry cell weight. Based on the phenotypic, chemotaxonomic, and phylogenetic characteristics, it has been determined that DM-R-R2A-13T represents a novel species belonging to the genus Massilia. As such, the name Massilia endophytica sp. nov. is proposed for this newly identified species. The type strain is DM-R-R2A-13T (= KCTC 92072T = GDMCC 1.2920T).

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Diversifying Polyhydroxyalkanoates: Synthesis, Properties, Processing and Applications

Gálvez

2023

Green-Based Nanocomposite Materials and Applications

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Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis

Cited by 40 publications

References 63 publications

Poly(hydroxyalkanoates): Production, Applications and End-of-Life Strategies–Life Cycle Assessment Nexus

Poly(hydroxyalkanoates): Production, Applications and End-of-Life Strategies–Life Cycle Assessment Nexus

Bioplastic (poly-3-hydroxybutyrate)-producing Massilia endophytica sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’

Diversifying Polyhydroxyalkanoates: Synthesis, Properties, Processing and Applications

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