Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics

Obruča, Stanislav; Dvořák, Pavel; Sedláček, Petr; Koller, Martin; Sedlář, Karel; Pernicová, Iva; Šafránek, David

doi:10.1016/j.biotechadv.2022.107906

Cited by 68 publications

(29 citation statements)

References 249 publications

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“…The success of industrial-scale PHA fermentations using lignocellulose is mainly dependent on four critical factors (i) cost-competitive PHA production, (ii) efficient utilization of lignocellulosic feedstock, (iii) high quality of PHA production, and (iv) green and environmentally friendly fermentation process for PHA production. ,− While the scientific community has been devoting its efforts to large-scale PHA production for over three decades, the high productivity and superior quality of PHA polyester are two critical factors determining its market applications . Conventional fermentation strategies such as discontinuous fermentation processes lead to the high production costs of PHAs.…”

Section: Emerging Fermentation Strategiesmentioning

confidence: 99%

Valorization of Lignocellulose by Producing Polyhydroxyalkanoates under Circular Bioeconomy Premises: Facts and Challenges

Andhalkar

Ahorsu

Marı́a³

et al. 2022

ACS Sustainable Chem. Eng.

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In light of the current concerns about environmental issues caused by the excessive use of fossil resources, more emphasis has been paid to the transition to a sustainable and circular economy. Bioplastics as eco-friendly products originating from biomass wastes have gained much attention to solve the problem of plastic pollution. Among them, polyhydroxyalkanoates (PHAs) are microbial polyesters produced using various feedstocks�renewable or recycled waste materials�contributing to a more sustainable commercial plastic life cycle by being a part of a circular bioeconomy. However, the scale-up of the PHA process cost effectively and sustainably remains challenging for large-scale industrial applications. This perspective provides a comprehensive overview of the current insights into lignocellulosic biomass's role in achieving a circular bioeconomy. Emerging greener biomass conversion technologies are discussed to characterize energy demand, cost, and sustainability within biorefinery PHA production. In addition, recent advances in synthetic biology and fermentation processes for PHA production are discussed. Technological challenges, i.e., bioreactor setup, downstream operation, and inconsistent properties to improve the sustainable production of PHAs and to help transfer this technology to real-world applications, are also addressed.

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Section: Emerging Fermentation Strategiesmentioning

confidence: 99%

Valorization of Lignocellulose by Producing Polyhydroxyalkanoates under Circular Bioeconomy Premises: Facts and Challenges

Andhalkar

Ahorsu

Marı́a³

et al. 2022

ACS Sustainable Chem. Eng.

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“…In this regard, the use of halophilic bacteria growing under high salinity has recently been proposed as a promising way to prevent contamination. However, metabolic tools to allow lactose conversion by these strains are currently lacking, while other challenges arise, including treatment of the saline effluent and corrosion of fermentation equipment (Obruča et al 2022 ).…”

Section: Phb Production From Industrial Biomass Side Streamsmentioning

confidence: 99%

Industrial side streams as sustainable substrates for microbial production of poly(3-hydroxybutyrate) (PHB)

Vlaeminck

Uitterhaegen

Quataert

et al. 2022

World J Microbiol Biotechnol

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Poly(3-hydroxybutyrate) (PHB) is a microbially produced biopolymer that is emerging as a propitious alternative to petroleum-based plastics owing to its biodegradable and biocompatible properties. However, to date, the relatively high costs related to the PHB production process are hampering its widespread commercialization. Since feedstock costs add up to half of the total production costs, ample research has been focusing on the use of inexpensive industrial side streams as carbon sources. While various industrial side streams such as second-generation carbohydrates, lignocellulose, lipids, and glycerol have been extensively investigated in liquid fermentation processes, also gaseous sources, including carbon dioxide, carbon monoxide, and methane, are gaining attention as substrates for gas fermentation. In addition, recent studies have investigated two-stage processes to convert waste gases into PHB via organic acids or alcohols. In this review, a variety of different industrial side streams are discussed as more sustainable and economical carbon sources for microbial PHB production. In particular, a comprehensive overview of recent developments and remaining challenges in fermentation strategies using these feedstocks is provided, considering technical, environmental, and economic aspects to shed light on their industrial feasibility. As such, this review aims to contribute to the global shift towards a zero-waste bio-economy and more sustainable materials.

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“…Further, T. taiwanensis genome possesses depolymerase encoded in phaZ gene and phaR gene known as transcriptional repressor. The phaR gene can be also found as a subunit of PHA synthase class IV where serves as an essential part of synthesis machinery [2]. The role of phaR gene seems to be also important for the PHA synthesis of other classes because the lack of this gene was shown to lead to increased or even stopped production of these granules [1].…”

Section: Butanoate Metabolism Pathwaymentioning

confidence: 99%

“…To decrease the costs of PHA production, one of the approaches is a synthesis by thermophilic bacteria. These bacteria are capable of living in higher temperatures and can produce PHA in these contamination free conditions [2]. The process can be then less cost demanding.…”

Section: Introductionmentioning

confidence: 99%

The Pathway Analysis of Tepidimonas taiwanensis LMG 22826T, Polyhydroxyalkanoate and Alkaline Protease Producer

Heřmánková¹,

Sedlář²

2022

Proceedings II of the 28st Conference STUDENT EEICT 2022: Selected Papers.

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The complete genome sequence of thermophilic type strain Tepidimonas tawanensis LMG 22826 T confirmed the earlier statement of its high biotechnological potential. Thanks to its reported ability to produce alkaline proteases and to synthesize polyhydroxyalkanoates (PHA), T. taiwanensis is worth our attention. Here, we present further descriptive analysis based on pathways detection which provides us better understanding of the core of such significant properties. This information can be then used in in silico modeling approaches. In total, 108 biological pathways were detected, phaCAB operon and granule-associated proteins -phasins were found and butanoate pathway comparison with other PHA producing bacteria was provided. Finally, simplified PHA synthesis pathway was introduced.

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Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics

Cited by 68 publications

References 249 publications

Valorization of Lignocellulose by Producing Polyhydroxyalkanoates under Circular Bioeconomy Premises: Facts and Challenges

Valorization of Lignocellulose by Producing Polyhydroxyalkanoates under Circular Bioeconomy Premises: Facts and Challenges

Industrial side streams as sustainable substrates for microbial production of poly(3-hydroxybutyrate) (PHB)

The Pathway Analysis of Tepidimonas taiwanensis LMG 22826T, Polyhydroxyalkanoate and Alkaline Protease Producer

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