Polyion Complex Micelles Composed of All-TranS Retinoic Acid and Poly (Ethylene Glycol)-Grafted-Chitosan

Jeong, Young‐Il; Kim, Sang-Hyo; Jung, Tae‐Young; Kim, In-Young; Kang, Sam-Suk; Jin, Yongdong; Ryu, Hyang‐Hwa; Sun, Honggang; Jin, Shu‐Guang; Kim, Kyung-keun; Ahn, Kyu-Youn; Jung, Shin

doi:10.1002/jps.20586

Cited by 81 publications

(48 citation statements)

References 29 publications

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“…25 Celecoxib was dissolved in 100% dimethylsulfoxide as a stock solution (40 mg/mL) and then diluted 200 times using minimum essential medium. The final dimethylsulfoxide concentration was maintained at less than 0.5% (v/v).…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

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Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Kim

Jeong²,

Jin³

et al. 2011

IJN

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Background: Celecoxib, a cyclo-oxygenase (COX)-2 inhibitor, has been reported to mediate growth inhibitory effects and to induce apoptosis in various cancer cell lines. In this study, we examined the potential effects of celecoxib on glioma cell proliferation, migration, and inhibition of COX-2 expression in vitro. Methods: Celecoxib was incorporated into poly DL-lactide-co-glycolide (PLGA) nanoparticles for antitumor drug delivery. Results: PLGA nanoparticles incorporating celecoxib had spherical shapes and their particle sizes were in the range of 50-200 nm. Drug-loading efficiency was not significantly changed according to the solvent used, except for acetone. Celecoxib was released from the PLGA nanoparticles for more than 2 days, and the higher the drug content, the longer the duration of drug release. PLGA nanoparticles incorporating celecoxib showed cytotoxicity against U87MG tumor cells similar to that of celecoxib administered alone. Furthermore, celecoxib did not affect the degree of migration of U87MG cells. PLGA nanoparticles incorporating celecoxib showed dose-dependent cytotoxicity similar to that of celecoxib alone in C6 rat glioma cells. Western blot assay of the C6 cells showed that neither celecoxib alone nor PLGA nanoparticles incorporating celecoxib affected COX-2 expression. Conclusion: PLGA nanoparticles incorporating celecoxib had antitumor activity similar to that of celecoxib alone, even though these particles did not affect the degree of migration or COX-2 expression in the tumor cells.

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Section: Cell Proliferation Assaymentioning

confidence: 99%

“…25 Briefly, the cell culture media was replaced with medium containing 5 mM hydroxyurea to eliminate any confounding effects of the experimental agents on cell proliferation. Twenty-four hours of treatment with hydroxyurea 5 mM resulted in complete inhibition of cell proliferation.…”

Section: Migration Assaymentioning

confidence: 99%

Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Kim

Jeong²,

Jin³

et al. 2011

IJN

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“…Nanocomplexes based on polyelectrolytes between anionic compounds and cationic compounds have been extensively investigated by several researchers. [24][25][26][27][28] Thünemann and Beyermann reported that retinoic acid as an anionic compound can form ion complexes with cationic polymers such as polyethyleneimine, and these complexes are nanosized carriers. 24 Furthermore, they also reported that another cationic polymer, poly(ethylene glycol)/poly(L-lysine) block copolymer, was also employed to form polyion complex micelles.…”

Section: Discussionmentioning

confidence: 99%

“…Jeong et al reported that retinoic acid and chitosan can form polyion complex micelles via interaction between amine groups of chitosan and carboxyl groups of retinoic acid, while chitosan itself does not form polymeric micelles. 26 In another report, they described nanoparticle formation based on ion complexes between hyaluronic acid and cisplatin. 27 Furthermore, they also reported that carboxymethyl (CM) chitosan can form nanoparticles via ion-complex formation between carboxyl groups of CM chitosan and amine groups of doxorubicin.…”

Section: Discussionmentioning

confidence: 99%

Hair dye-incorporated poly-γ-glutamic acid/glycol chitosan nanoparticles based on ion-complex formation

Lee¹,

Jeong²,

Choi³

2011

IJN

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Background: p-Phenylenediamine (PDA) or its related chemicals are used more extensively than oxidative hair dyes. However, permanent hair dyes such as PDA are known to have potent contact allergy reactions in humans, and severe allergic reactions are problematic. Methods: PDA-incorporated nanoparticles were prepared based on ion-complex formation between the cationic groups of PDA and the anionic groups of poly(γ-glutamic acid) (PGA). To reinforce PDA/PGA ion complexes, glycol chitosan (GC) was added. PDA-incorporated nanoparticles were characterized using field-emission scanning electron microscopy, Fourier-transform infrared (FT-IR) spectroscopy, dynamic light scattering, and powder X-ray diffractometry (XRD). Results: Nanoparticles were formed by ion-complex formation between the amine groups of PDA and the carboxyl groups of PGA. PDA-incorporated nanoparticles are small in size (,100 nm), and morphological observations showed spherical shapes. FT-IR spectra results showed that the carboxylic acid peak of PGA decreased with increasing PDA content, indicating that the ion complexes were formed between the carboxyl groups of PGA and the amine groups of PDA. Furthermore, the intrinsic peak of the carboxyl groups of PGA was also decreased by the addition of GC. Intrinsic crystalline peaks of PDA were observed by XRD. This crystalline peak of PDA was completely nonexistent when nanoparticles were formed by ion complex between PDA, PGA, and GC, indicating that PDA was complexed with PGA and no free drug existed in the formulation. During the drug-release experiment, an initial burst release of PDA was observed, and then PDA was continuously released over 1 week. Cytotoxicity testing against HaCaT human skin keratinocyte cells showed PDA-incorporated nanoparticles had lower toxicity than PDA itself. Furthermore, PDA-incorporated nanoparticles showed reduced apoptosis and necrosis reaction at HaCaT cells. Conclusion:The authors suggest that these microparticles are ideal candidates for a vehicle for decreasing side effects of hair dye.

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“…Thus, RAFC exhibited anti-invasive effect on prostrate cells by inhibition of the EMT process via Hedgehog signaling pathway. (Jeong, et al 2006) and acute promyelocytic leukemia (Chung, 2012). However, the promising biological activity of RA is limited by its poor aqueous solubility (0.1 µM at pH 7.3) under in vivo conditions (Crocetti, et al 2012;Tanaka, et al 2013).…”

Section: Introductionmentioning

confidence: 99%

A novel retinoic acid chalcone reverses epithelial‑mesenchymal transition in prostate cancer cells

Zhong

Hou

2015

Bangladesh J Pharmacol

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Polyion Complex Micelles Composed of All-TranS Retinoic Acid and Poly (Ethylene Glycol)-Grafted-Chitosan

Cited by 81 publications

References 29 publications

Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Hair dye-incorporated poly-γ-glutamic acid/glycol chitosan nanoparticles based on ion-complex formation

A novel retinoic acid chalcone reverses epithelial‑mesenchymal transition in prostate cancer cells

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Polyion Complex Micelles Composed of All-TranS Retinoic Acid and Poly (Ethylene Glycol)-Grafted-Chitosan

Cited by 81 publications

References 29 publications

Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Hair dye-incorporated poly-&gamma;-glutamic acid/glycol chitosan nanoparticles based on ion-complex formation

A novel retinoic acid chalcone reverses epithelial‑mesenchymal transition in prostate cancer cells

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Hair dye-incorporated poly-γ-glutamic acid/glycol chitosan nanoparticles based on ion-complex formation