Polymerase chain reaction assay for verifying the labeling of meat and commercial meat products from game birds targeting specific sequences from the mitochondrial D-loop region

Abstract

A PCR assay was developed for the identification of meats and commercial meat products from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), guinea fowl (Numida meleagris), pigeon (Columba spp.), Eurasian woodcock (Scolopax rusticola), and song thrush (Turdus philomelos) based on oligonucleotide primers targeting specific sequences from the mitochondrial D-loop region. The primers designed generated specific fragments of 96, 100, 104, 106, 147, 127, and 154 bp in length for quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock, and song thrush tissues, respectively. The specificity of each primer pair was tested against DNA from various game and domestic species. In this work, satisfactory amplification was accomplished in the analysis of experimentally pasteurized (72 degrees C for 30 min) and sterilized (121 degrees C for 20 min) meats, as well as in commercial meat products from the target species. The technique was also applied to raw and sterilized muscular binary mixtures, with a detection limit of 0.1% (wt/wt) for each of the targeted species. The proposed PCR assay represents a rapid and straightforward method for the detection of possible mislabeling in game bird meat products.

Mitochondrial and nuclear markers for the authentication of partridge meat and the specific identification of red-legged partridge meat products by polymerase chain reaction

“…Species-specific PCR (Rojas et al, 2010a) or realtime PCR assays (Rojas et al, 2010b) have also been reported previously for the identification of partridge meat. The reported PCR technique using species-specific primers (Rojas et al, 2010a) was suitable for the analysis of heat-treated partridge meat products and admixed meats.…”

“…The reported PCR technique using species-specific primers (Rojas et al, 2010a) was suitable for the analysis of heat-treated partridge meat products and admixed meats. However, this technique was capable of detecting partridge species only from the genus Alectoris because it was based in the use of primers targeting a fragment from the mitochondrial D-loop gene conserved among partridge species of this genus.…”

Authentication of meat and commercial meat products from common pigeon (Columba livia) woodpigeon (Columba palumbus) and stock pigeon (Columba oenas) using a TaqMan® real-time PCR assay

“…including two or more species in their composition, because results obtained after digestion of the PCR products might show a combination of miscellaneous restriction patterns representing all the possible species included in the adulterated sample. Species-specific PCR (Haunshi et al, 2009;Rojas et al, 2010a), or real-time PCR assays (Rojas et al, 2010b) have also been previously reported for the identification of pigeon meat. These techniques are suitable for the analysis of heat-treated pigeon meat products and admixed meats.…”

Loop‐Mediated Isothermal Amplification (LAMP): A Rapid and Sensitive Tool for Quality Assessment of Meat Products

“…PCR‐based techniques that have been used for animal species identification include PCR‐sequencing of DNA barcodes (Ferri and others ), PCR‐restriction fragment length polymorphism (PCR‐RFLP; Murugaiah and others ; Rojas and others ), PCR‐random amplified polymorphic DNA (RAPD; Rastogi and others ), PCR with species‐specific primers (Rojas and others ), and real‐time PCR (Okuma and Hellberg ). The RFLP is a long‐duration method which often results in ambiguous band patterns that are difficult to interpret.…”