Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Zhuang, Liying; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu

doi:10.1186/s12917-019-1774-3

Cited by 28 publications

(16 citation statements)

References 37 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With advances in molecular technologies, novel assays for CPV-2 detection have been developed and established. Combined with lateral flow strips (LFSs) and magnetic purification, the PCR-LFS assay detect CPV-2 in 80 min with high sensitivity while omitting the electrophoresis step for analyzing results (22). The direct TaqMan real-time PCR method was also established to diagnose CPV-2 and CDV in dogs without the requirement of nucleic acid extraction (23).…”

Section: Discussionmentioning

confidence: 99%

An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection

Wang

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

With increasing complications of canine parvovirus infection cases, disease diagnosis and treatment have become more difficult. In this study, specificity primers for the conserved region of the VP2 gene of canine parvovirus 2 (CPV-2) were synthesized and evaluated. An improved polymerase cross-linking spiral reaction (PCLSR) method for early and rapid diagnosis of CPV-2 was established. The results showed that the amplification reaction was optimal when run at 62 • C for 50 min and could be used to detect CPV-2 without any cross-reactions with other pathogens of canine infectious diseases. Reaction results were directly judged by the naked eyes, with the positive amplification tube shown as luminous yellow and the negative tube as bright purple. Compared with the previously reported polymerase spiral reaction (PSR) method for CPV-2 detection, this reaction was performed using improved primer pairs and a better dye identification method (using an indicator comprising phenol red and cresol red). The detection limit of PCLSR was 3.9 × 10 1 copies using gel electrophoresis or a visible dye. The positive rate of 132 clinical samples was 42.42%, which was identically the same as that of the PSR method and slightly higher than that of the colloidal gold strip method (39.39%). The newly developed CPV-PCLSR assay shows the advantage of rapid visualization of results and offers a convenient and rapid method for early CPV-2 diagnosis with higher sensitivity and specificity than the established methods.

show abstract

Section: Discussionmentioning

confidence: 99%

An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection

Wang

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Recently, a combination of LFI assay and other technologies, such as PCR ( 172 ), RT-LAMP ( 173 ), RT-RPA ( 155 , 174 – 176 ), and quantum dots ( 177 ), for the diagnosis of animal pathogens has also been explored. Therefore, the current approach in the development of a desirable FMD diagnostic test typically involves the incorporation of two assays such as RT-LAMP-LFD ( 46 ) and RT-RPA-LFD ( 155 , 156 ).…”

Section: Chromatographic Strip Testsmentioning

confidence: 99%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

View full text Add to dashboard Cite

Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

show abstract

“…For instance, Broughton et al developed a CRISPR-Cas12-based lateral flow assay for detection of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 from extracted patient sample RNA within 40 min (Broughton et al, 2020). A PCR-based lateral flow assay was established for the detection of canine parvovirus 2 with detection limit of 30 copies/µL (Zhuang et al, 2019). In addition, Li et al developed a LAMP-based lateral flow biosensor for rapid detection of Brucella spp.…”

Section: Lateral Flow Stripsmentioning

confidence: 99%

Recent Trends in Nanomaterial-Based Biosensors for Point-of-Care Testing

Wang¹,

Guo

2020

Front. Chem.

View full text Add to dashboard Cite

In recent years, nanomaterials of different shape, size, and composition have been prepared and characterized, such as gold and silver nanoparticles, quantum dots, mesoporous silica nanoparticles, carbon nanomaterials, and hybrid nanocomposites. Because of their unique physical and chemical properties, these nanomaterials are increasingly used in point-of-care testing (POCT) to improve analytical performance and simplify detection process. They are used either as carriers for immobilizing biorecognition elements, or as labels for signal generation, transduction and amplification. In this commentary, we highlight recent POCT technologies that employ nanotechnology for the analysis of disease biomarkers, including small-molecule metabolites, enzymes, proteins, nucleic acids, cancer cells, and pathogens. Recent advances in lateral flow tests, printable electrochemical biosensors, and microfluidics-based devices are summarized. Existing challenges and future directions are also discussed.

show abstract

Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Cited by 28 publications

References 37 publications

An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection

An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection

Advances in the Diagnosis of Foot-and-Mouth Disease

Recent Trends in Nanomaterial-Based Biosensors for Point-of-Care Testing

Contact Info

Product

Resources

About