Polymerase chain reaction compatibility of adhesive transfer tape based microfluidic platforms

Nath, Pulak; Maity, Tuhin; Pettersson, Frida Ekholm; Resnick, Jesse M.; Kunde, Yuliya A.; Kraus, Noelle; Castaño, Nicolas

doi:10.1007/s00542-013-1901-1

Cited by 16 publications

(12 citation statements)

References 22 publications

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“…Leaching studies have not been performed by us or by the manufacturer of this particular adhesive and may be necessary for some applications. However, a number of PSA films have been used for PCR, DNA and electrophoresis applications with good results, for example those by Nath et al (2014), Pjescic and Crews (2012) and Greer et al (2007). Solvents do attack both the adhesive film and a number of plastics.…”

Section: Device Robustnessmentioning

confidence: 97%

“…The term was introduced in 2005, when Bartholomeusz et al presented the patterning of different materials using a cutting plotter, and their application in electroplating, shadow masking, PDMS micromolding and laminated microfluidics (Bartholomeusz et al 2005). Since then, a number of microfluidic devices have been implemented using this technique (for example, Nath et al 2014;Pjescic and Crews 2012;Greer et al 2007;Atencia et al 2012;Kido et al 2007;Gorking et al 2010), including several works by our group (Martinez-Duarte et al 2010. The use of xurography to pattern adhesive films has been particularly meaningful to the microfluidics community since robust, inexpensive devices can be fabricated in matter of minutes without the need for a cleanroom.…”

Section: Introductionmentioning

confidence: 99%

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A study on the limits and advantages of using a desktop cutter plotter to fabricate microfluidic networks

Islam

Natu

Martínez-Duarte

2015

Microfluid Nanofluid

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a low cost, flexible and quick process to fabricate microchannels keeps increasing.Historically, the majority of the initial microfluidics work was done using glass-or silicon-made microfluidic devices (Harrison et al. 1992;Wilding et al. 1994;Woolley and Mathies 1994;Jiang et al. 1995) using techniques like wet and dry etching. In the mid-1990s, a revolution in the fabrication of microfluidics took place with the advent of soft lithography, where a master mold is used to make poly-dimethylsiloxane (PDMS) replicas by casting (Dong et al. 1996;Xia and Whitesides 1998;Duffy et al. 1998). Soft lithography is now a standard for microfluidic prototyping, in part because it enables a relatively inexpensive and rapid fabrication. As long as a master mold is available, experimental microfluidics devices can be made in few hours without the need for a cleanroom. However, the master molds are still mostly fabricated in a cleanroom following a number of processes including dry etching of silicon, and especially SU-8 photolithography (Martinez-Duarte and Madou 2011). As expected, the resolution and achievable complexity of the master mold depend highly on the choice of fabrication technique. For example, microchannels with features of even 100 nm are possible to fabricate using electron beam lithography (Rogers and Nuzzo 2005; Alom Ruiz and Chen 2007). However, most microfluidic devices would feature dimensions from the tens of micrometers to several hundreds, achievable with conventional SU-8 photolithography.Other relatively inexpensive fabrication techniques are printer based, such as that presented by Carrilho et al. (2009) who selectively deposited hydrophobic wax on regular paper to create channel walls. In this particular case, the cross section of the channel is not empty, but contains fibers that facilitate the wicking of the sample throughout the fluidic network. Bruzewicz et al. (2008) also demonstrated a Abstract In this paper, we focus on characterizing the limits of xurography, or patterning with a razor blade, of a pressure-sensitive double-sided adhesive. This is a rapid, inexpensive technique to fabricate robust microfluidics devices. Straight, curved and square serpentine as well as zigzag channels of different dimensions are studied. General guidelines are provided to assess feasibility of a particular geometry a priori. The mechanics of the cut are explored with the aim at identifying the bottlenecks that limit the maximum resolution achieved in xurography of adhesive films. A number of advantages and disadvantages of this technique compared to other common fabrication techniques are also provided.

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Section: Device Robustnessmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

A study on the limits and advantages of using a desktop cutter plotter to fabricate microfluidic networks

Islam

Natu

Martínez-Duarte

2015

Microfluid Nanofluid

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“…The microfluidic circuit (channels, chambers, manifolds) is patterned in a middle layer, channels are enclosed with top and bottom capping layers, sealed by solvent bonding [ 86 ], ultrasonic welding [ 87 ] thermal-pressure bonding [ 88 ], or adhesives, wax bonding [ 89 ], and double-sided tapes [ 90 ]. Since many adhesives and tapes [ 91 ], as well as some chip materials [ 92 , 93 , 94 ], are known to impede amplification, testing of all chip materials for compatibility with amplification is a must. Incompatibility may be due adsorption of reagents or dyes on surfaces, or chemical inhibition of enzymes.…”

Section: Chip Fabricationmentioning

confidence: 99%

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

et al. 2018

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Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges (‘chips’) that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification; pre-stored, paraffin-encapsulated lyophilized reagents; and real-time or endpoint optical detection are described. These chips can be used with a companion module for separating plasma from blood through a combined sedimentation-filtration effect. Three reporter types: Fluorescence, colorimetric dyes, and bioluminescence; and a new paradigm for end-point detection based on a diffusion-reaction column are compared. Multiplexing (parallel amplification and detection of multiple targets) is demonstrated. Low-cost detection and added functionality (data analysis, control, communication) can be realized using a cellphone platform with the chip. Some related and similar-purposed approaches by others are surveyed.

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“…These benefits come at the expense of higher capital equipment costs, the requirement for a vacuum pump to clear out debris and fumes, and potential burn residue created during the cutting [45]. While material leaching of plastics and adhesives may pose problems for some sensitive biological assays, oftentimes burn products from laser cutting of particular materials have been shown to inhibit reactions such as PCR [46]. Plotter cutters (also commonly referred to as vinyl cutters or cutting plotters) are significantly cheaper, require no pumping system, and leave no burn residues.…”

Section: Maker Microfluidics Manufacturingmentioning

confidence: 99%

Enabling Microfluidics: from Clean Rooms to Makerspaces

Walsh

Kong

Murthy

et al. 2017

Trends in Biotechnology

148

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The traditional requirement for clean rooms and specialized skills has inhibited many biologists from pursuing new microfluidic innovations. Makerspaces provide a growing alternative to clean rooms: they provide low-cost access to fabrication equipment such as laser cutters, plotter cutters, and 3D printers; use commercially available materials; and attract a diverse community of product designers. This Opinion discusses the materials, tools, and building methodologies particularly suited for developing novel microfluidic devices in these spaces, with insight into biological applications and leveraging the maker community. The lower barrier to access of makerspaces ameliorates the otherwise poor accessibility and scalability of microfluidic prototyping.

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Polymerase chain reaction compatibility of adhesive transfer tape based microfluidic platforms

Cited by 16 publications

References 22 publications

A study on the limits and advantages of using a desktop cutter plotter to fabricate microfluidic networks

A study on the limits and advantages of using a desktop cutter plotter to fabricate microfluidic networks

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

Enabling Microfluidics: from Clean Rooms to Makerspaces

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