Polymerase Chain Reaction for Detection of Endocervical Chlamydia trachomatis Infection in Women Attending a Gynecology Outpatient Department in India

Singh, Vineeta; Rastogi, Sangita; Garg, Suneela; Kapur, Sujala; Kumar, Amit; Salhan, Sudha; Mittal, Aliza

doi:10.1159/000326874

Cited by 28 publications

(24 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, as uSpA is a forme fruste of ReA, and taken together, both ReA as well as uSpA constitute the seronegative spondyloarthropathy group, patients categorized as uSpA by a rheumatologist were also included in the study. Furthermore, despite a high prevalence of genital C. trachomatis in our country [17][18][19][20][21][22][23][24][25], this pathogen appears to be overlooked in seronegative spondyloarthropathies such as ReA and uSpA in India. In order to find the magnitude of the problem, we investigated patients with ReA or uSpA in a case-control study by molecular and nonmolecular methods for the intra-articular presence of C. trachomatis MOMP, plasmid genes, and local or systemic anti-chlamydial IgA or IgG antibodies.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Prabhash

Bhakuni

Rastogi

2014

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

Introduction: There is a paucity of information on the frequency of Chlamydia trachomatis-induced reactive arthritis (ReA) and undifferentiated spondyloarthropathy (uSpA) in India. In this study, arthritic patients suffering from ReA, uSpA, and rheumatoid arthritis (RA) were screened to investigate the presence of C. trachomatis infection in the synovial fluid (SF) or serum by molecular and nonmolecular methods. Methodology: A total of 76 arthritic patients with ReA (n = 16) and uSpA (n = 22) composed the study group while those with RA (n = 38) served as controls. The detection of C. trachomatis DNA was done by semi-nested PCR (snPCR) and nested PCR (nPCR) targeting two different genes of C. trachomatis, namely major outer membrane protein and plasmid, respectively. The presence of serum or SF immunoglobulin IgG and IgA antibodies against C. trachomatis was studied by commercial enzyme-linked immunosorbent assay kits. Results: The SF from 9 of 38 (23.6%) patients (5 with ReA and 4 with uSpA) was positive for at least one C. trachomatis DNA by snPCR or nPCR in comparison to RA (1/38 [2.6%]; p value < 0.05). There was no correlation between the snPCR or nPCR and the serological results of patients with ReA or uSpA. Conclusions: As molecular diagnostic techniques established intra-articular C. trachomatis infection among this group of seronegative spondyloarthropathies in India, these findings should be viewed with concern, and snPCR or nPCR should be considered for a more reliable diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Prabhash

Bhakuni

Rastogi

2014

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reports have shown that risk factors for Chlamydia trachomatis infection are: Age 18-27 years, non-use of contraceptives, frequent post-coital bleeding, painful maturation, urban residence, low income, cervical infection, polygamous marriage and mucopurulent cervical discharge (Singh et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

<i>Chlamydia trachomatis</i> in Women with Ful-Term Deliveries and Women with Abortion

Jahromi¹

2010

American Journal of Infectious Diseases

View full text Add to dashboard Cite

Problem statement: There are some documents which support the role of some certain infections such as Chlamydia trachomatis in spontaneous abortion. As there were not data about role this bacterium in abortion in this area of IRAN, this study was conducted to evaluate the prevalence of Chlamydia trachomatis in women with abortion and compare it with healthy women with no previous history of abortion. Approach: This case-control study was carried out in Shariatee hospital of Hormozgan University of medical sciences, during [2004][2005]. A number of 220 women with definite diagnosis of previous abortion and 200 matched women with normal full term delivery and negative history of miscarriage as controls were studied as case and control groups. All obtained PAP smears from the case and the control groups were then tested using Immunoflourescent method for detection of Chlamydia trachomatis. Data was analyzed, using SPSS software (chi square and t-test). Results: The prevalence of positive direct immunofluorescent test on PAP smears indicating the presence of Chlamydia trachomatis was 56 (25.45%) in women with abortion comparing to 13 (5.20%) in women in control group, the difference was significant (p = 0.0001). Conclusion: This study showed Chlamydia trachomatis is an important causative agent for abortion in this area of IRAN.

show abstract

“…Urine provides a reliable, effective and non-invasive sample for detecting Chlamydia trachomatis. 19 We tested 210 urine samples from pregnant women. No sample was positive for Chlamydia trachomatis either using our in-house PCR or a Gen-kam PCR kit.…”

Section: Discussionmentioning

confidence: 99%

Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women

Hassanzadeh

Sharifi²,

Bazargani³

et al. 2012

Microbiol Res (Pavia)

View full text Add to dashboard Cite

Chlamydia trachomatis is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with Chlamydia trachomatis reaches 100 million annually. The sensitivity and specificity of nacid amplification tests are 95% and 99%, respectively. Urine samples can provide a non-invasive method of testing for the detection of Chlamydia trachomatis by polymerase chain reaction (PCR). The aim of this study was to evaluate the prevalence of Chlamydia trachomatis infection in pregnant women referred to a teaching hospital affiliated to Shiraz University of Medical Sciences. Urine samples were obtained from 210 pregnant women and investigated microscopically and macroscopically by urinalysis. Precipitants were also used for DNA extraction and PCR test for detecting Chlamydia trachomatis. Among 210 urine specimens from women aged 15-39 years, none were positive for Chlamydia trachomatis by PCR. In spite of the high sensitivity and specificity of PCR, and the elimination of inhibitory effects on PCR test, no pregnant woman was positive for Chlamydia trachomatis. Here, we suggest that a larger sample should be studied and other sensitive methods could also be used in the future.

show abstract

Polymerase Chain Reaction for Detection of Endocervical Chlamydia trachomatis Infection in Women Attending a Gynecology Outpatient Department in India

Cited by 28 publications

References 17 publications

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

<i>Chlamydia trachomatis</i> in Women with Ful-Term Deliveries and Women with Abortion

Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women

Contact Info

Product

Resources

About