Polymerase chain reaction for detection of <i>Ureaplasma diversum</i> from urogenital swabs in cattle in Australia

Smith, A; Chousalkar, Kapil K.; Chenoweth, PC

doi:10.1111/j.1751-0813.2012.00939.x

Cited by 11 publications

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“…as mais frequentemente isoladas nos casos de distúrbios reprodutivos. A presença de Mollicutes em muco vulvovaginal de vacas pode ocorrer sem associação com surtos de doença reprodutiva (Petit et al 2007, Smith et al 2012, no entanto, quando isolados em muco cervical e/ou sistema reprodutor superior estão relacionados a síndrome reprodutiva caracterizados por repetição de cio, infertilidade, endometrites, abortos, nascimento de bezerros fracos entre outros (Ruhnke et al 1984, Petit et al 2007.…”

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Ocorrência de Mollicutes e Ureaplasma spp. em surto de doença reprodutiva em rebanho bovino no Estado da Paraíba

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“…Cardoso et al (2006) demonstraram que o muco prepucial é um material biológico que pode ser utilizado para detecção de Mollicutes e Smith et al (2012) encontraram uma frequência de 35,2% de suabes do sistema urogenital positivos para U. diversum em animais sem lesões reprodutivas.…”

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Ocorrência de Mollicutes e Ureaplasma spp. em surto de doença reprodutiva em rebanho bovino no Estado da Paraíba

et al. 2013

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“…Diagnosis and detection of U. diversum infection has traditionally relied on culturing techniques, although polymerase chain reaction (PCR) has been increasingly used to confirm the presence of Ureaplasma . Recently, detection of U. diversum directly from clinical swabs of Australian cattle was reported . The current study was designed to establish the presence of U. diversum in the Australian cattle population by using culture and PCR…”

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Presence of Ureaplasma diversum in the Australian cattle population

2013

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In cattle, Ureaplasma diversum has been associated with decreased fertility, granular vulvovaginitis, endometritis, salpingitis and spontaneous abortion in cows and seminal vesiculitis, balanoposthitis and changes in bull sperm. The presence of U. diversum within the Australian cattle population has not been established. One of the aims of this study was to determine if U. diversum was present in Australian cattle, using culture and polymerase chain reaction (PCR), both of which are considered to be gold standards for bacterial identification. Of 100 samples collected from 66 male and 34 female cattle, 15 were positive for U. diversum. Therefore, Australia can no longer be considered free of U. diversum. Further studies should be conducted to ascertain the effects of U. diversum within Australian cattle herds and, if warranted, to investigate prevention, treatment and eradication protocols.

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“…The next study also concludes that more work is needed, this time on the pathogenic role of Ureaplasma diversum in Australian cattle . The first identification of the organism by PCR in Australia was in female cattle in 2010, and its effect on cattle health and productivity is unclear. This study, by the same group, aimed to confirm the infection status of U. diversum in Australian bulls using PCR analyses and culture of the organism, and to identify any morphological changes of sperm from infected bulls.…”

Section: Production Animalsmentioning

confidence: 86%

In this issue – November 2013

Jackson¹

2013

Aust Veterinary J

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Small animalsThe first paper this month is a retrospective look at 17 cases of cutaneous adverse food reactions in cats that were presented to a dermatology referral service. 1 Each case was confirmed by a clear worsening of the skin of the cats when they were re-challenged with normal food after completing an elimination diet. They then had to improve once the test diet was repeated. Cats typically presented with severe, perennial, non-seasonally flaring pruritus. Home-prepared elimination diets were completed by 16 cats and 8 cats had initial poor response to minimum 6-week commercial hydrolysed protein diets.The adverse foods that were identified included fish, chicken, beef, commercial dry, and some canned foods. The authors discuss the challenges of diagnosing the condition, as there had to be strict adherence to the dietary trials. They discuss the limitations of their study, including that the response was based on the owners' observations and veterinary interpretation, rather than objective measures. As many cats have other concurrent dermatoses, responses to these trials may be very difficult to unravel. The next paper looks at the haematological and biochemical profiles of 192 sighthounds including Whippet, Greyhound, Italian Greyhound, Sloughi, Saluki, Borzoi, Pharaoh Hound and Azawakh breeds. These were compared with non-breed-specific laboratory reference intervals. 2 bs_bs_banner EDITORIAL EDITORIAL

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Polymerase chain reaction for detection of Ureaplasma diversum from urogenital swabs in cattle in Australia

Abstract: The present test enabled detection of the organism directly from clinical swabs collected from animals with or without lesions.

Cited by 11 publications

References 6 publications

Ocorrência de Mollicutes e Ureaplasma spp. em surto de doença reprodutiva em rebanho bovino no Estado da Paraíba

Ocorrência de Mollicutes e Ureaplasma spp. em surto de doença reprodutiva em rebanho bovino no Estado da Paraíba

Presence of Ureaplasma diversum in the Australian cattle population

In this issue – November 2013

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