Polymerase chain reaction (PCR) amplification of bacilliform virus (RV‐PJ) DNA in Penaeus japonicus Bate and systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon Fabricius

Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes

“…The culture water was first chlorinated with 25 ppm of sodium hypochlorite and dechlorinated by vigorous aeration. Before starting the experiment, the shrimps were randomly selected and screened by PCR for WSSV infection using the primer having the base pair of 643 designed by Takahashi et al [19].…”

“…The collected haemolymph samples of both experimental and control groups, and blank control were checked by WSSV diagnostic PCR (two steps) using the primer designed by Takahashi et al [19]. The DNA extraction and PCR amplification followed the method described by Chang et al [20].…”

White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

“…The shrimp were fed artificial pelleted feed (35% protein). The experimental animals were screened (20 random shrimps from a group of 200) for white spot virus using PCR (Takahashi et al 1996). After screening, a total of 120 healthy animals were used in this experiment.…”

“…Template DNA was extracted following the procedures of Vijayan et al (1998) from the eyestalks (without compound eye) and pleopods removed from infected and control shrimp at various time intervals. The PCR was carried out according to Takahashi et al (1996) to detect WSSV infection. PCR products were electrophoresed in 0.8% agarose gel stained with ethidium bromide, and visualized under ultraviolet transillumination.…”

Safety and protective effect of a disinfectant (STEL water) for white spot syndrome viral infection in shrimp

“…Gross signs of infection include abnormal red discoloration of the body and white spots within the cuticle. Several diagnostic methods including PCR, in situ hybridization, electron microscopy, and histopathology have been developed for WSSV (Lo et al 1996, Takahashi et al 1996, Lu et al 1997, Nunan et al 1997, Park et al, 1998, Tapay et al 1999. However, no good therapeutic treatment has yet been established for its control, and preventive measures are the only recommended methods of protection against it (Flegel et al 1997).…”