Polymerase chain reaction-restriction fragment length polymorphism method for differentiation of uropathogenic specific protein gene types

Lai, Yun Mei; Zaw, Myo Thura; Shamsudin, Shamsul Bahari; Wang, Qisheng

doi:10.1016/j.jmii.2015.06.002

Cited by 4 publications

(5 citation statements)

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“…In this study, we found that the positive rate of double J tube bacterial culture of patients in both groups was higher than that of bladder and urine bacterial culture. Furthermore, the bacteria cultured in double J tubes were mainly GNB, Escherichia coli , followed by GPC, which was consistent with previous research results [ 52 , 54 , 55 ]. Combined with above data, it can be considered that continuous indwelling catheterization can reduce the number of bacteria colonized in the double J tube, thus reducing the incidence of urinary tract infection.…”

Section: Discussionsupporting

confidence: 92%

Effects of Continuous Catheterization on Reducing Postoperative Urinary Tract Infection in Cervical Cancer Patients with Double J Stent Placement

Liu

Yao

Xing

et al. 2021

Journal of Healthcare Engineering

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This study aims at exploring the effect of continuous catheterization on reducing postoperative urinary tract infection in cervical cancer patients with double J tube placement. To be specific, a retrospective analysis was performed on 120 cases of cervical cancer patients who underwent laparoscopic or open radical hysterectomy in Shengjing Hospital of China Medical University from January to December 2019. They were divided into a persistent group (n = 70) and a short-term group (n = 50) according to indwelling catheter time. The incidence of postoperative complications and the positive rate of bacterial culture in bladder urine and double J tube bacterial culture were compared between the two groups. As a result, it was found that the incidence of postoperative fever and urinary tract infection in the short-term group was significantly higher than that in the persistent group ( P < 0.05 ). There was no significant difference in the incidence of postoperative hematuria, bladder stimulation, and urinary system injury between the two groups. The positive rate of double J tube bacterial culture in both groups was also proved to be higher than that in bladder culture, and the difference was statistically significant ( P < 0.05 ). And in the short-term group ( P < 0.05 ), the difference in the positive rate of bladder culture between the two groups was not statistically significant. To conclude, we found that continuous catheterization can reduce the incidence of postoperative urinary tract infection in cervical cancer patients with double J tube placement, which might be helpful for the treatment of cervical cancer.

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Section: Discussionsupporting

confidence: 92%

Effects of Continuous Catheterization on Reducing Postoperative Urinary Tract Infection in Cervical Cancer Patients with Double J Stent Placement

Liu

Yao

Xing

et al. 2021

Journal of Healthcare Engineering

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“…PAIs consist typically of VAGs encoded within chromosomal regions and are capable of being exchanged via horizontal gene transfer [52]. Among our isolates, we detected malX and usp, located on PAI I CFT073 and PAI usp , in 100% and 75.9% of the isolates, respectively [53,54]. Usp has been associated with UTI isolates and is suggested as a potential VAG for use as a molecular epidemiological marker specific for UPEC.…”

Section: Discussionmentioning

confidence: 77%

Genotypic Characterization of Uropathogenic Escherichia coli from Companion Animals: Predominance of ST372 in Dogs and Human-Related ST73 in Cats

Aurich,

Wolf,

Prenger-Berninghoff

et al. 2023

Antibiotics

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Extraintestinal pathogenic Escherichia coli (ExPEC) account for over 80% and 60% of bacterial urinary tract infections (UTIs) in humans and animals, respectively. As shared uropathogenic E. coli (UPEC) strains have been previously reported among humans and pets, our study aimed to characterize E. coli lineages among UTI isolates from dogs and cats and to assess their overlaps with human UPEC lineages. We analysed 315 non-duplicate E. coli isolates from the UT of dogs (198) and cats (117) collected in central Germany in 2019 and 2020 utilizing whole genome sequencing and in silico methods. Phylogroup B2 (77.8%), dog-associated sequence type (ST) 372 (18.1%), and human-associated ST73 (16.6%), were predominant. Other STs included ST12 (8.6%), ST141 (5.1%), ST127 (4.8%), and ST131 (3.5%). Among these, 58.4% were assigned to the ExPEC group and 51.1% to the UPEC group based on their virulence associated gene (VAG) profile (ExPEC, presence of ≥VAGs: papAH and/or papC, sfa/focG, afaD/draBC, kpsMTII, and iutA; UPEC, additionally cnf1 or hlyD). Extended-spectrum cephalosporin (ESC) resistance mediated by extended-spectrum β-lactamases (ESBL) and AmpC-β-lactamase was identified in 1.9% of the isolates, along with one carbapenemase-producing isolate and one isolate carrying a mcr gene. Low occurrence of ESC-resistant or multidrug-resistant (MDR) isolates (2.9%) in the two most frequently detected STs implies that E. coli isolated from UTIs of companion animals are to a lesser extent associated with resistance, but possess virulence-associated genes enabling efficient UT colonization and carriage. Detection of human-related pandemic lineages suggests interspecies transmission and underscores the importance of monitoring companion animals.

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“…They encode virulence proteins such as toxins, adhesins, and invasins, which allow bacteria to colonize and damage host tissues. The presence of the USP virulence gene in such a high proportion of E. coli isolates suggests an elevated potential for pathogenicity and the ability to cause disease in the context of wound and burn infections [3]. Because females spend the majority of their time in the kitchen, where accidents are common, the incidence of burns was higher in females.…”

Section: Discussionmentioning

confidence: 99%

“…Journal for Research in Applied Sciences and Biotechnology www.jrasb.com stay than surgical wounds [3]. Infection is a major cause of morbidity and mortality in hospitalized burn patients [4,5].…”

Section: Issn: 2583-4053mentioning

confidence: 99%

Molecular Analysis of Some Virulence Genes of Escherichia Coli Isolates from Wound and Burn Samples in Kirkuk City

Magthab

2023

J. Res. Appl. Sci. Biotechnol.

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Background: Gram-negative infections caused by non-spore producing E. coli that are facultatively anaerobic have been recognized as serious infections linked to higher mortality and significant costs associated with medical care. Aim: The goal of this study was to determine whether or not a number of E. coli strains are capable of producing virulence genes by using a technique called real time PCR. Materials and Methods: 155 people with burns and wounds had their cultures obtained, and susceptibility testing using swabs, tissue biopsies, and needle aspirations were performed. When compared to other techniques of specimen collection, swab cultures yield precise results and are a practicable, widely utilized, non-invasive, and cost-effective method. The two most recommended techniques for obtaining specimens are tissue biopsy and needle aspiration. Swab cultures, on the other hand, are appropriate since they are useful, widely applied, non-invasive, and reasonably priced. Samples from patients were collected in the consultation rooms, including swabs from burns and wounds. The sufferers had these taken from them. Before being identified, smear samples were initially cultivated in the lab on several selective media. Transport media-filled sterile collection containers containing these samples were brought to the lab. Results: According to the study's findings, E. coli was found in 27.10% of the burns and wounds samples. According to the study's findings, the majority of patients with E. coli infections (71.43 percent) were females, while only 28.57 percent were males. According to the study's findings, 28.57 percent of patients diagnosed with E. coli infections were between the ages of 31 and 40. The investigation also indicated that all of the sputum samples containing E. coli isolates were from patients above the age of 40. Furthermore, 45 percent of urine samples were collected from patients aged 31 to 40, and 42.86 percent of patients had wound infections. When examined using the MTP technique, fifty percent of the E. coli isolates tested positive for being potent biofilm producers, according to the study's findings. The DNA of 12 distinct E. coli bacterial isolates was isolated and purified using a genomic DNA purification kit. The bacteria were cultured on chromogenic agar medium to produce these E. coli isolates. The results were detected using electrophoresis on agarose gel at a concentration of 1.5 percent. The gel was then exposed to ultraviolet light, which revealed the DNA as compact bands. The virulence gene was found in 95.24 percent of the E. coli isolates taken from burn and wound sites, according to the study's findings. The majority of E. coli isolates tested positive for amikacin, imipenem, and gentamycin, according to the study's findings. The majority of E. coli isolates, on the other hand, were resistant to the medications amoxicillin, levofloxacin, cefotaxime, ceftriaxone, pefloxacin, cefepime, and ampicillin.

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Polymerase chain reaction-restriction fragment length polymorphism method for differentiation of uropathogenic specific protein gene types

Cited by 4 publications

References 10 publications

Effects of Continuous Catheterization on Reducing Postoperative Urinary Tract Infection in Cervical Cancer Patients with Double J Stent Placement

Effects of Continuous Catheterization on Reducing Postoperative Urinary Tract Infection in Cervical Cancer Patients with Double J Stent Placement

Genotypic Characterization of Uropathogenic Escherichia coli from Companion Animals: Predominance of ST372 in Dogs and Human-Related ST73 in Cats

Molecular Analysis of Some Virulence Genes of Escherichia Coli Isolates from Wound and Burn Samples in Kirkuk City

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