Polymeric and polymer-supported pseudostationary phases in micellar electrokinetic chromatography: Performance and selectivity

Palmer, Christopher P.

doi:10.1002/1522-2683(200012)21:18<4054::aid-elps4054>3.0.co;2-f

Cited by 61 publications

(19 citation statements)

References 98 publications

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“…More recently, micelle polymers have appeared in the literature for enantiomeric separations [41,42]. In contrast to conventional micelles, micelle polymers are formed via covalent bonds and do not have a CMC.…”

Section: Micellesmentioning

confidence: 99%

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

Kahle

Foley

2007

Electrophoresis

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Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKCThe separation of enantiomers using electrokinetic chromatography (EKC) with chiral microemulsions is comprehensively reviewed through December 1, 2006. Aqueous chiral EKC separations based on other pseudostationary phases such as micelles and vesicles or on other chiral selectors such as CDs, crown ethers, glycopeptides, ligand exchange moeities are also reviewed from both mechanistic and applications perspective for the period of

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Section: Micellesmentioning

confidence: 99%

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

Kahle

Foley

2007

Electrophoresis

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“…In 1994, Wang and Warner [70] were the first to report the use of a polymeric surfactant added to the BGE in MEKC. Polymeric surfactants offer several distinct advantages over conventional micelles [63,[71][72][73]. Firstly, polymerization of the surfactant eliminates the dynamic equilibrium due to the formation of covalent bonds between the surfactant aggregates.…”

Section: Methods Development and Validationmentioning

confidence: 99%

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Kapnissi‐Christodoulou¹

2012

Electrophoresis

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In addition, hydrogen bonding, dipole-dipole, and dispersive interactions can contribute to the solute partitioning between the two phases [4][5][6].CE was originally considered as a powerful analytical tool for the analysis of biological macromolecules. It has though, over the years, been extensively used for the separation of other compounds, such as chiral drugs, food additives, pesticides, inorganic ions, organic acids, and others. In this chapter, the ability of CE, and particularly CZE and MEKC, to be used for the qualitative and quantitative determination of compounds in pharmaceutical, biological and natural samples is investigated. In each approach, a number of studies are reported and discussed. These studies involve establishment of optimum separation conditions, method validation, optimization of sample-preparation procedure and application for the determination of the analytes under study in real samples. The first part of this chapter involves the determination of polyphenolic compounds using CZE with UVVis detector in red and white wines, while the second part involves the determination of pharmaceutical compounds in biological samples, such as blood and urine, using the hyphenated technique CE-MS (mass spectrometry). The third and final part emphasizes the importance of MEKC in chiral analysis since it has been known that usually only one enantiomer is active, while the other may be less active, inactive or has adverse effects. Determination of polyphenolic compounds in natural samplesPolyphenolic compounds exist in a variety of natural products, such as fruits, vegetables, beverages (tea, wine and juices), honey, cacao and herbs. They attract a lot of interest due to their beneficial implication in human health. They have been widely studied due to their antioxidant capacity and their association with several pathological conditions, such as hypertension, cardiovascular disease, dementia, and even cancer [7][8][9]. Therefore, due to their health significance, numerous analytical methods have, over the last decades, been developed for their separation, identification and quantitation in natural products [10][11][12][13].According to literature, the simplest CE method, CZE, proved to be the best method for the determination of polyphenolic compounds in wine samples [14][15][16][17]. In such studies, and in each case, when the optimum CZE method was applied to different red and white wines, it was established that red wines have higher levels of polyphenolic compounds than white wines and that the polyphenolic composition varies among different wines.

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“…Different kinds of detergents could be used depending on purposes in many aspects such as gel electrophoresis, micellar electrokinetic chromatography (MEKC), ion pair reagents of high performance liquid chromatography and solubilization/extraction of proteins, peptides, DNA, etc . Among all available detergents, sodium dodecyl sulphate (SDS) is one of the most popular anionic surfactants in biological research because of its unique usefulness in polyacrylamide gel electrophoresis (PAGE) to cause protein denaturation and to give the protein molecule net charge densities depending on their masses .…”

Section: Introductionmentioning

confidence: 99%

Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI‐MS)

Mandal

Chen

et al. 2011

J. Mass. Spectrom.

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Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents.

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Polymeric and polymer-supported pseudostationary phases in micellar electrokinetic chromatography: Performance and selectivity

Cited by 61 publications

References 98 publications

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI‐MS)

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