Polymeric hepatitis C virus non-structural protein 5A nanocapsules induce intrahepatic antigen-specific immune responses

Fichter, Michael; Piradashvili, Keti; Pietrzak-Nguyen, Anette; Pretsch, Leah; Kühn, G; Strand, Susanne; Knuf, Markus; Zepp, Fred; Wurm, Frederik R.; Mailänder, Volker; Landfester, Katharina; Gehring, Stephan

doi:10.1016/j.biomaterials.2016.08.046

Cited by 18 publications

(18 citation statements)

References 59 publications

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“…injections is unconventional due to high immunogenicity but particularly suitable to target liver DCs for hepatic infections. Fichter et al developed nanocapsules (NC) exclusively synthesized of HCV NS5A antigen via miniemulsion polymerization to avoid unspecific immune responses caused by additional carrier substances and to increase the amount of delivered antigen. Monophosphoryl lipid A (MPLA, a synthetic TLR4 agonist recently approved by US and European FDA for the use in HBV) was further adsorbed onto the NC surface.…”

Section: Nanomaterial‐based Immunotherapy Targeting the Livermentioning

confidence: 99%

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Nanomaterial Manipulation of Immune Microenvironment in the Diseased Liver

Huang

2018

Adv Funct Materials

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The liver contains intricate immunological mechanisms, capable of inducing both immune activation and suppression for the maintenance of a homeostatic immune environment. From an immunological point of view, chronic liver disease is indeed a result of the imbalance of pro-and anti-inflammatory responses. Therefore, application of immunotherapy in liver diseases is logical and contains a huge potential for future development. Despite rapid advances in immunotherapy, research at the interdisciplinary interface of immunology and material science is important for further enhancement of immunotherapeutic efficiency and reduction of side effects. Moreover, the liver is particularly suitable for the application of nanomaterial-based immunotherapy due to its biological filtration function to sequester a majority of administrated nanoparticles from systemic circulation. Here, an insight of advanced liver diseases and how liver cells exert their immune functions in the diseased liver is summarized first to provide basic principles for therapeutic application, followed by an overview of the latest studies on nanomaterial-based immunotherapy for liver diseases.

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Section: Nanomaterial‐based Immunotherapy Targeting the Livermentioning

confidence: 99%

Section: Nanomaterial‐based Immunotherapy Targeting the Livermentioning

confidence: 99%

Nanomaterial Manipulation of Immune Microenvironment in the Diseased Liver

Huang

2018

Adv Funct Materials

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“…The preparation of nanocontainers with a hydrophilic core from water-in-oil miniemulsions and their subsequent transfer to aqueous medium is of special importance, since it enables the efficient encapsulation of hydrophilic payloads in large quantities. For the development of vaccine formulations against hepatitis C virus (HCV) infection the NS5A protein was used [16]. The major advantages of synthetic particulate vaccines are the size and surface properties of the nanocapsules that can be adjusted accurately [17], the co-administration of vaccine-antigen and -adjuvant and thus the possibility to target certain cells of the immune system [18,19].…”

Section: Advantages Of Nanoparticulate Vaccine Constructsmentioning

confidence: 99%

“…Thus, the primary entry side of viral infections, such as HBV and HCV, can be targeted subsequently resulting in an intrahepatic immune response. In recent studies, the intravenous immunization of mice with NS5A-NCs induced intrahepatic antigen-specific CD4 + T-cell immune responses determined by the secretion on IFN-γ and IL-2 [16]. The induction of T H 1-responses, an essential prerequisite for the treatment of chronic infections, was additionally achieved through the inclusion of MPLA and other adjuvants, such as R848, in combination with IFN-γ.…”

Section: Specific Advantages Of Hbv Nanocapsulesmentioning

confidence: 99%

Novel Strategies in Vaccine Design: Can Nanocapsules Help Prevent and Treat Hepatitis B?

Gehring

Pietrzak-Nguyen

Fichter

et al. 2017

Nanomedicine (Lond.)

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“…The authors also emphasized that an essential advantage of using nanocarriers made solely of antigen is the lack of induction of an immune response directed against the additional material present in nanocarriers providing viral antigen. 109 Finally, polymeric nanocarriers can be useful in immunotherapy, aiming to induce immune tolerance to allergens (antigens). Briefly, this therapy involves supplying the body with a small amount of antigen to inhibit the synthesis of the underlying allergy molecules, namely IgE class antibodies and cytokines such as IL-4, IL-5, and IL-13.…”

mentioning

confidence: 99%

<p>Biomedical Applications of Multifunctional Polymeric Nanocarriers: A Review of Current Literature</p>

Karabasz¹,

Bzowska²,

Szczepanowicz³

2020

IJN

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Polymeric nanomaterials have become a prominent area of research in the field of drug delivery. Their application in nanomedicine can improve bioavailability, pharmacokinetics, and, therefore, the effectiveness of various therapeutics or contrast agents. There are many studies for developing new polymeric nanocarriers; however, their clinical application is somewhat limited. In this review, we present new complex and multifunctional polymeric nanocarriers as promising and innovative diagnostic or therapeutic systems. Their multifunctionality, resulting from the unique chemical and biological properties of the polymers used, ensures better delivery, and a controlled, sequential release of many different therapeutics to the diseased tissue. We present a brief introduction of the classical formulation techniques and describe examples of multifunctional nanocarriers, whose biological assessment has been carried out at least in vitro. Most of them, however, also underwent evaluation in vivo on animal models. Selected polymeric nanocarriers were grouped depending on their medical application: anti-cancer drug nanocarriers, nanomaterials delivering compounds for cancer immunotherapy or regenerative medicine, components of vaccines nanomaterials used for topical application, and lifestyle diseases, ie, diabetes.

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Polymeric hepatitis C virus non-structural protein 5A nanocapsules induce intrahepatic antigen-specific immune responses

Cited by 18 publications

References 59 publications

Nanomaterial Manipulation of Immune Microenvironment in the Diseased Liver

Nanomaterial Manipulation of Immune Microenvironment in the Diseased Liver

Novel Strategies in Vaccine Design: Can Nanocapsules Help Prevent and Treat Hepatitis B?

<p>Biomedical Applications of Multifunctional Polymeric Nanocarriers: A Review of Current Literature</p>

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