Polymerization-based signal amplification for paper-based immunoassays

Badu‐Tawiah, Abraham K.; Lathwal, Shefali; Kaastrup, Kaja; Al‐Sayah, Mohammad H.; Christodouleas, Dionysios C.; Smith, Barbara; Whitesides, George M.; Sikes, Hadley D.

doi:10.1039/c4lc01239a

Cited by 97 publications

(117 citation statements)

References 18 publications

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“…LOD was defined as the minimum concentration that yielded an average test area intensity that was exceeded by 3 times the standard deviation of a blank (sample without antigen) over the average intensity of the blank 26 . Molarity of LOD was determined using a molecular weight of 50 kD for Dengue NS1 27 .…”

Section: Methodsmentioning

confidence: 99%

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays

Tam

Puig

Yen

et al. 2017

Journal of Immunoassay and Immunochemistry

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Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.

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Section: Methodsmentioning

confidence: 99%

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays

Tam

Puig

Yen

et al. 2017

Journal of Immunoassay and Immunochemistry

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“…The maximum frequency at which a difference between the control and HIV samples was observed was 1 KHz with streptavidin while the chips without streptavidin only had a maximum frequency for separation of 500 Hz. The performance and sensitivity of the paper chips can further be enhanced by optimizing the surface chemistry of the chips using chemical immobilisation of the antibodies as shown by others [17,18]. …”

Section: Hiv Detectionmentioning

confidence: 97%

A graphene-modified cellulose paper microchip for HIV detection

Safavieh

Khetani

Kaul

et al. 2015

Advances in Global Health Through Sensing Technologies 2015

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Rapid and inexpensive virus detection and quantification at the point-of-care is of paramount importance for HIV management in resource-limited settings. Here, we report on an easy-to-fabricate, cellulose paperbased microchip with printed graphene-modified electrodes for rapid detection of HIV-1 through electrical sensing. We evaluated the effect of electrode material and geometry on the performance of the microchip to detect serially diluted, electrically conductive samples. We evaluated the optimized microchip with HIVspiked samples.

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“…For instance, regular commercial papers have been used to fabricate immunoassay analyzer, microfluidic devices and paper-based electronics [29][30][31]. In this research, we developed a paper-based electrodeposition chip for the gel generation.…”

Section: Discussionmentioning

confidence: 99%

Paper-Based Electrodeposition Chip for 3D Alginate Hydrogel Formation

et al. 2015

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Hydrogel has been regarded as one significant biomaterial in biomedical and tissue engineering due to its high biocompatibility. This paper proposes a novel method to pattern calcium alginate hydrogel in a 3D way via electrodeposition process based on a piece of paper. Firstly, one insulating paper with patterned holes is placed on one indium tin oxide (ITO) glass surface, which is put below another ITO glass. Then, 1% sodium alginate solution with 0.25% CaCO 3 nano particles is filled between these two glasses. In the bottom glass, patterns of electrodes followed patterns of holes on the insulating layer. Hydrogel forms on patterned electrodes when electrochemical potential is applied due to electrodeposition. The experiments demonstrate that the pattern of alginate hydrogels follows the pattern of electrodes exactly. In addition, the hydrogel's height is controllable by applied potential and reaction time. An equivalent circuit model and a hydrogel growth model have been built to predict the electrodeposition current and hydrogel's growth. This method for gel formation is easy and cheap since the main material is one piece of insulated paper, which provides an easy and controllable method for 3D hydrogel patterning.

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Polymerization-based signal amplification for paper-based immunoassays

Cited by 97 publications

References 18 publications

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays

A graphene-modified cellulose paper microchip for HIV detection

Paper-Based Electrodeposition Chip for 3D Alginate Hydrogel Formation

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