Polymers in Tissue Engineering

Heise, Rebecca L.; Blakeney, Bryan A.; Pouliot, Robert A.

doi:10.1007/978-3-319-12478-0_7

Cited by 4 publications

(2 citation statements)

References 161 publications

(209 reference statements)

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“…This indicated that Alphastatin played a part in the ionic cross-linking. In a strict definition, the particle size of the nanomaterial should be between 1 and 100 nm [16], but the diameter of the nanoparticles made by polymer material may be larger than 100 nm, ranging from 10 to 500 nm, and cannot exceed 1000 nm [17]. In this study, AsCs NPs at the mass ratio of 2 : 1 were still nanoparticles since their average particle size was 387 4 ± 12 5 nm.…”

Section: The Effect Of the Cs/alphastatin Ratio On Characteristicsmentioning

confidence: 81%

Alphastatin-Loaded Chitosan Nanoparticle Preparation and Its Antiangiogenic Effect on Lung Carcinoma

Zhang¹,

Hu²

2019

International Journal of Polymer Science

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Alphastatin is a 24-amino acid peptide and can suppress tumor angiogenesis by inhibiting both the migration and tubule formation of vascular endothelial cells. However, the anticancer effect of Alphastatin is limited due to the short half-life and degradation in the body. In this study, Alphastatin-loaded chitosan nanoparticles (AsCs NPs) were prepared with an initial concentration of 2 mg/ml for chitosan and 1 mg/ml for Alphastatin. AsCs NPs presented the encapsulation efficiency of 32.4%, the mean particle size of 387.4 nm, the polydispersity index of 0.223, and the zeta potential of +28.1 mV. AsCs NPs have a sustained release for 6 days and were stable in serum for at least 24 hours. And the NPs could preserve the integrity of encapsulated Alphastatin and released Alphastatin for 24 hours. In a subcutaneous LA975 lung carcinoma xenograft T739 mouse model, AsCs NPs significantly inhibited the tumor growth, tumor volume, and microvessel density (MVD), and the antitumor effect was even stronger than that of Alphastatin. In addition, the VEGF-induced tube formation of HUVEC could be inhibited by AsCs NPs in vitro and the serum containing AsCs NPs, and the protein level of SphK1 in HUVEC was also decreased by AsCs NPs, suggesting an inhibitory effect of AsCs NPs on the SphK1-S1P signaling pathway. Furthermore, hemolysis assay showed a safety on blood compatibility of AsCs NPs. Our study indicated that AsCs NPs inhibited the SphK1-S1P signaling pathway and enhanced the antiangiogenic effect of Alphastatin both in vitro and in vivo.

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Section: The Effect Of the Cs/alphastatin Ratio On Characteristicsmentioning

confidence: 81%

Alphastatin-Loaded Chitosan Nanoparticle Preparation and Its Antiangiogenic Effect on Lung Carcinoma

Zhang¹,

Hu²

2019

International Journal of Polymer Science

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“…[6, 13] Our method for creating these fibers can utilize a variety of photosensitive polymers which can be robust and tuned for biodegradability. [14] Here we demonstrate the patterning of HeLa cells in poly(ethylene glycol) diacrylate (PEGDA) and methacrylated gelatin (GelMA) [15] solutions, and confirm high cell viability after fiber extraction.…”

mentioning

confidence: 79%

Surface Acoustic Waves Grant Superior Spatial Control of Cells Embedded in Hydrogel Fibers

Lata

Guo

et al. 2016

Advanced Materials

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By exploiting surface acoustic waves and a coupling layer technique, cells are patterned within a photosensitive hydrogel fiber to mimic physiological cell arrangement in tissues. The aligned cell-polymer matrix is polymerized with short exposure to UV light and the fiber is extracted. These patterned cell fibers are manipulated into simple and complex architectures, demonstrating feasibility for tissue-engineering applications.

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