Polymorphism Candidate Genes of Indigenous Lien Minh Chickens

Abstract

Lien Minh chicken is an indigenous breed with several favorable properties, such as high productivity and good meat quality, and is associated with the economic development of the people in the Lien Minh village, Cat Hai, Hai Phong. The objective of the current research was to investigate the single nucleotide polymorphisms (SNPs) of candidate genes, which might be associated with broodiness and egg production traits. Ninety Lien Minh chicken individuals were genotyped for five SNPs of chicken prolactin (PRL), Vasoactive Intestinal Peptide (VIP), neuropeptide Y gene (NPY), growth hormone (GH), and growth hormone receptor (GHR) genes. Blood samples were used for DNA extraction and then for genotyping by the PCR-RFLP method. The allele frequencies obtained were as follows: 0.19 and 0.81 for alleles C and T (PRL-C2161G), respectively; in VIP (G5138982T), 0.55 for the G allele and 0.45 for T; in GH-SacI (the intron 4), 0.02 for A and 0.98 for B; and in GHR-NspI, 0.82 for C and 0.18 for T. The NPY gene (four nucleotide indel) had the frequencies of 0.86 for I and 0.14 for D. The four studied polymorphic loci (PRL, VIP, NPY, and GH) were in Hardy-Weinberg equilibrium in the Lien Minh chicken population. These are the initial results, which can be used to analyze the correlation of molecular markers and egg production traits in Lien Minh chickens.

Genetic structure in natural populations of Dacrydium elatum (Roxb.) Wall. (Podocarpaceae) in the Central Highlands of Vietnam inferred by Microsatellites

“…Ho > He were detected of four populations in the present study, suggesting that these populations are predominantly allogamic, which might be a result of outcrossing and reflecting the population structures. Although, it has previously been studied on the genetic diversity of D. elatum.. Tran et al [16] had compared the effectiveness between ISSR and SSR markers in assessing genetic diversity of natural populations of D. elatum in Tay Nguyen, Vietnam and show that high level of genetic variation of D. elatum (He = 0.301). This result was consistent with the study of Tran et al [16].…”

“…Although, it has previously been studied on the genetic diversity of D. elatum.. Tran et al [16] had compared the effectiveness between ISSR and SSR markers in assessing genetic diversity of natural populations of D. elatum in Tay Nguyen, Vietnam and show that high level of genetic variation of D. elatum (He = 0.301). This result was consistent with the study of Tran et al [16]. Several reports have shown that high genetic diversity in other conifer using SSR markers [11,12,[27][28][29][30][31][32] However, low of genetic diversity were found in prior studies [4,33,34].…”

“…Many SSR markers were developed and have been widely used to probe genetic diversity within and among conifer populations [11][12][13]. Previous studies investigated the genetic variation and verified the taxonomic status of the Dacrydium species at the molecular level, such as D. pectinatum [14], D. imbricatus [15], D. elatum [16], however the genetic structure of D. elatum has not been explored in Vietnam. Meanwhile, knowing genetic diversity is important for the development of conservation strategies and the sustainable utilization of thisspecies [6,17].…”