Polymorphism of the Md-Exp7 gene for the biosynthesis of expansin in wild species of the genus Malus Mill.

Savel’ev, N. I.; Shamshin, I. N.; Савельева, Н. Н.; Лыжин, А. С.

doi:10.1134/s2079059715030156

Cited by 2 publications

(3 citation statements)

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“…In previously studied Russian apple cultivars collection the most common were genotypes homozygous for allele 202 and 198 : 202 genotypes (Savel'ev et al, 2014a). At the same time, analysis of Md-Exp7 locus polymorphism in the Malus species representing four sections of the genus, showed that allele 202 was the most common and the authors suggested that this allele was the oldest one, while in species of section Malus that includes M. domestica, the most common were alleles 198, 202 and 204 bp (Savel'ev et al, 2014b).…”

Section: Discussionmentioning

confidence: 99%

“…The allelic composition of studied apple cultivars for the SSR marker was associated with differences in fruit softening: 198 bp (best firmness retention), 202 bp (intermediate) and 214 bp (worst) (Costa et al, 2008). Later screening of apple cultivars and species collections revealed some rare Md-Exp7 alleles with unknown effect on fruit softening (Nybom et al, 2012(Nybom et al, , 2013Savel'ev et al, 2014b).…”

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Ethylene and expansin biosynthesis related genes polymorphism in local apple (Malus domestica Borkh.) cultivars from VIR Collection of plant genetic resources

et al. 2018

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At Pushkin and Pavlovsk Laboratories of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) a diverse collec tion of local apple cultivars is maintained. Some of the cultivars are widely used in breeding programs for their ecological plasticity, increased adaptation to abiotic stress and disease resistance, still there have been no large-scale studies of these local cultivars for fruit storage ability. Fruit softening during storage is an important problem for apple production. Retention of desirable firmness after prolonged storage is one of the key requirements for new apple cultivars. Expansin and ethy lene biosynthesis related genes are known to be involved in control of fruit softening in apple, and gene specific molecular markers have been reported. In this study the polymorphism and allelic configuration of ethylene and expansin biosynthesis related genes Md-ACS1, Md-ACO1 and Md-Exp7 involved in control of fruit softening in 87 local apple cultivars from VIR Collection of Plant Genetic Resources were analyzed. PCR markers Md-ACS1, Md-ACO1 and SSR-marker Md-Exp7 were used in the study. The allele frequencies in the collection generally coincided with the data from previous studies. Md-ACS1 allele 2 associated with reduced ethylene production was found only in three local cultivars, while all the studied local cultivars were heterozygous for the Md-ACO1 locus, as well as most modern Russian apple cultivars. Half of the studied local cultivars were heterozygous for Md-Exp7 (198 : 202). Thirteen local cultivars with rare Md-Exp7 alleles (206, 210 and 212) were detected. No association was found between the Md-Exp7 genotype and the cultivars’ maturation time. The obtained results can be used for additional evaluation of the cultivars’ potential for breeding.

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Section: Discussionmentioning

confidence: 99%

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Ethylene and expansin biosynthesis related genes polymorphism in local apple (Malus domestica Borkh.) cultivars from VIR Collection of plant genetic resources

et al. 2018

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“…These genes have been mapped, the effect of allelic variants of genes on the level of endogenous ethylene synthesis in fruits and, accordingly, on the storage quality of fruits, has been established, and effective DNA markers for identifying alleles have been developed (Sunako et al, 1999;Oraguzie et al, 2004;Costa et al, 2005). Using these markers, allelic combinations in the breeding material and collection samples of the apple tree were assessed in the world (Oraguzie et al, 2007;Zhu, Barritt, 2008;Nybom et al, 2012;Suprun, Tokmakov, 2013;Savel'ev et al, 2014b;Lyzhin, Savelyeva, 2020;Shamshin et al, 2020). The influence of the Md-ACS3a gene on the synthesis of endogenous ethylene in fruits was also revealed (Bai et al, 2012).…”

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Identification of apple genes <i>Md-Exp7</i> and <i>Md-PG1</i> alleles in advanced selections resistant to scab

et al. 2022

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The creation of apple varieties with a high level of f lesh f irmness and long shelf life is one of the important goals in breeding. Among the genes controlling these traits, the role of the endogenous ethylene biosynthesis control gene, Md-ACS1, the expansin gene, Md-Exp7, and the polygalacturonase gene, Md-PG1, has been established. The use of DNA marker analysis to solve problems in breeding for fruit quality traits allows one not only to track several target genes simultaneously, but also to cull plants with undesirable alleles at the early stages of development. In order to select complex donors of breeding traits, molecular genetic identif ication of the genes that determine the quality traits of apple fruits Md-Exp7 and Md-PG1 was performed in 256 breeding selections carrying the scab resistance gene Rvi6 and valuable allelic variants of the Md-ACS-1 gene, which determines the endogenous synthesis of ethylene in fruits: 90 samples with the Md-ACS1 allele (2/2) and 166 samples with Md-ACS1 (1/2). As a result of the study, an allelic combination for the Md-Exp7 and Md-PG1 genes was established. Analysis of the parental cultivars (Renet Simirenko, Modi, Smeralda, Renoir, Fulzhion and Granny Smith) used to obtain hybrid selections revealed three alleles 198, 202, 214 bp according to the DNA marker of the Md-Exp7 gene. The SSR marker for the Md-PG1 gene amplif ied three alleles (289, 292, 298 bp) on the abovementioned cultivars. Within the 256 breeding selections samples that have the most priority for breeding alleles of the desired genes in combination with the Rvi6 gene and/or with selection-priority allelic variants of the Md-ACS-1 gene were identif ied. Of the most valuable for breeding, 46 accessions carrying the combination Md-Exp7 (202:202) + Md-ACS1 (2/2) were distinguished. Hybrids with alleles Md-PG1 (292:292) + Md-ACS1 (2/2) are also most valuable for use in breeding and as donors of selection-valuable alleles; 21 samples were identif ied. Accessions with a complex of breeding-valuable target alleles are valuable complex donors, as well as valuable breeding material for creating varieties with improved fruit quality characteristics and scab resistance.

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Polymorphism of the Md-Exp7 gene for the biosynthesis of expansin in wild species of the genus Malus Mill.

Cited by 2 publications

References 5 publications

Ethylene and expansin biosynthesis related genes polymorphism in local apple (Malus domestica Borkh.) cultivars from VIR Collection of plant genetic resources

Ethylene and expansin biosynthesis related genes polymorphism in local apple (Malus domestica Borkh.) cultivars from VIR Collection of plant genetic resources

Identification of apple genes <i>Md-Exp7</i> and <i>Md-PG1</i> alleles in advanced selections resistant to scab

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