Polymorphism of the ND1 and CO1 Mitochondrial Genes in Populations of Liver Fluke Fasciola hepatica

Morozova, Еlena V.; Chrisanfova, G. G.; Архипов, И А; Semyenova, S. K.

doi:10.1023/b:ruge.0000036534.01952.19

Cited by 13 publications

(8 citation statements)

References 10 publications

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“…This is largely in agreement with previous reports on genetic variability in mitochondrial genes nad 1 and cytochrome oxidase 1 of F. hepatica by other researchers [45–50]. Cwiklinski et al [12] concluded that the F. hepatica genome is highly heterogeneous.…”

Section: Discussionsupporting

confidence: 92%

Identity of Fasciola spp. in sheep in Egypt

et al. 2016

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BackgroundIn Egypt, liver flukes, Fasciola spp. (Digenea: Fasciolidae), have a serious impact on the farming industry and public health. Both Fasciola hepatica and Fasciola gigantica are known to occur in cattle, providing the opportunity for genetic recombination. Little is known on the identity and genetic variability of Fasciola populations in sheep.MethodsThis study was performed to determine the prevalence of liver flukes in sheep in Menofia Province as a representative area of the delta region in Egypt, as measured by postmortem examination of slaughtered animals at three abattoirs. The identity and genetic variability of Fasciola spp. in slaughtered animals were determined by PCR-sequence analysis of the nuclear ribosomal internal transcribed spacer 1 (ITS1) and the mitochondrial NADH dehydrogenase subunit 1 (nad1) genes.ResultsPhysical inspection of the liver indicated that 302 of 2058 (14.7%) slaughtered sheep were infected with Fasciola spp. Sequence analysis of the ITS1 and nad1 genes of liver flukes from 17 animals revealed that 11 animals were infected with F. hepatica, four with F. gigantica, and two with both species. Seventy eight of 103 flukes genetically characterized from these animals were F. hepatica, 23 were F. gigantica, and two had ITS1 sequences identical to F. hepatica but nad1 sequences identical to F. gigantica. nad1 sequences of Egyptian isolates of F. gigantica showed pronounced differences from those in the GenBank database. Egyptian F. gigantica haplotypes formed haplogroup D, which clustered in a sister clade with haplogroups A, B and C circulating in Asia, indicating the existence of geographic isolation in the species.ConclusionsBoth F. hepatica and F. gigantica are prevalent in sheep in Egypt and an introgressed form of the two occurs as the result of genetic recombination. In addition, a geographically isolated F. gigantica population is present in the country. The importance of these observations in epidemiology of fascioliasis needs to be examined in future studies.

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Section: Discussionsupporting

confidence: 92%

Identity of Fasciola spp. in sheep in Egypt

et al. 2016

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“…Our study also showed that the majority of perch were infected by several T. clavata haplotypes (Fig. 2).The latter result would indicate continual infection by differ, ent haplotypes that coexist in the same lakes -a result also observed for other eye ukes [84,85].…”

Section: Humic Lakes As Eye Parasite-free Environmentssupporting

confidence: 82%

Humic-acid-driven escape from eye parasites revealed by RNA-seq and target-specific metabarcoding

Noreikienė

Ozerov²,

Ahmad³

et al. 2020

Preprint

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Background Next generation sequencing (NGS) technologies are extensively used to dissect the molecular mechanisms of host-parasite interactions in human pathogens. However, ecological studies have yet to fully exploit the power of NGS as a rich source for formulating and testing new hypotheses. Methods We studied Eurasian perch (Perca fluviatilis) and its eye parasite (Trematoda, Diplostomidae) communities in fourteen lakes that differed in humic content in order to explore host-parasite-environment interactions. We hypothesised that high humic content along with low pH would decrease the abundance of the intermediate hosts (gastropods), thus limiting the occurrence of diplostomid parasites in humic lakes. This hypothesis was initially invoked by whole eye RNA-seq data analysis and subsequently tested using PCR-based detection and a novel targeted metabarcoding approach.Results Whole eye transcriptome results revealed over expression of immune-related genes and the presence of eye parasite sequences in RNA-seq data obtained from perch living in clear-water lakes. Both PCR-based and targeted-metabarcoding approach showed that perch from humic lakes were completely free from diplostomid parasites, while the prevalence of eye flukes in clear-water lakes that contain low amounts of humic substances was close to 100%, with the majority of NGS reads assigned to Tylodelphys clavata. Conclusions High intraspecific diversity of T. clavata indicates that massively parallel sequencing of naturally pooled samples represents an efficient and powerful strategy for shedding light on cryptic diversity of eye parasites. Our results demonstrate that perch populations in clear-water lakes experience contrasting eye parasite pressure compared to those from humic lakes, which is reflected by prevalent differences in the expression of immune-related genes in the eye. This study highlights the utility of NGS to discover novel host-parasite-environment interactions and provide unprecedented power to characterize the molecular diversity of cryptic parasites.

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“…Our study also showed that the majority of perch were infected by several T. clavata haplotypes (Fig. 2).The latter result would indicate continual infection by differ, ent haplotypes that co-exist in the same lakes -a result also observed for other eye ukes [84,85].…”

Section: Humic Lakes As Eye Parasite-free Environmentssupporting

confidence: 81%

Humic-acid-driven escape from eye parasites revealed by RNA-seq and target-specific metabarcoding

Noreikienė

Ozerov²,

Ahmad³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundNext generation sequencing (NGS) technologies are extensively used to dissect the molecular mechanisms of host-parasite interactions in human pathogens. However, ecological studies have yet to fully exploit the power of NGS as a rich source for formulating and testing new hypotheses. MethodsWe studied Eurasian perch (Perca fluviatilis) and its eye parasite (Trematoda, Diplostomidae) communities in fourteen lakes that differed in humic content in order to explore host-parasite-environment interactions. We hypothesised that high humic content along with low pH would decrease the abundance of the intermediate hosts (gastropods), thus limiting the occurrence of diplostomid parasites in humic lakes. This hypothesis was initially invoked by whole eye RNA-seq data analysis and subsequently tested using PCR-based detection and a novel targeted metabarcoding approach.ResultsWhole eye transcriptome results revealed over expression of immune-related genes and the presence of eye parasite sequences in RNA-seq data obtained from perch living in clear-water lakes. Both PCR-based and targeted-metabarcoding approach showed that perch from humic lakes were completely free from diplostomid parasites, while the prevalence of eye flukes in clear-water lakes that contain low amounts of humic substances was close to 100%, with the majority of NGS reads assigned to Tylodelphys clavata. ConclusionsHigh intraspecific diversity of T. clavata indicates that massively parallel sequencing of naturally pooled samples represents an efficient and powerful strategy for shedding light on cryptic diversity of eye parasites. Our results demonstrate that perch populations in clear-water lakes experience contrasting eye parasite pressure compared to those from humic lakes, which is reflected by prevalent differences in the expression of immune-related genes in the eye. This study highlights the utility of NGS to discover novel host-parasite-environment interactions and provide unprecedented power to characterize the molecular diversity of cryptic parasites.

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Polymorphism of the ND1 and CO1 Mitochondrial Genes in Populations of Liver Fluke Fasciola hepatica

Cited by 13 publications

References 10 publications

Identity of Fasciola spp. in sheep in Egypt

Identity of Fasciola spp. in sheep in Egypt

Humic-acid-driven escape from eye parasites revealed by RNA-seq and target-specific metabarcoding

Humic-acid-driven escape from eye parasites revealed by RNA-seq and target-specific metabarcoding

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