Polymorphism UCP2 Gene in Predicting the Occurrence of Type 2 Diabetes Mellitus in Respondents Cohort Study of Risk Factors for Non-communicable Diseases in Bogor, West Java, Indonesia

Abstract

Diabetes mellitus type 2 (DM) is a risk factor for other non-communicable diseases and is a problem, along with the increasing prevalence in Indonesia. One of the proteins involved in the occurrence of diabetes is UCP2, which is produced by the UCP2 gene. So that the polymorphism in this gene is likely to affect the incidence of diabetes. This study uses a cross-sectional method in a population study cohort of risk factors for noncommunicable diseases (RF-NCD) conducted in the city of Bogor, West Java, Indonesia. This analysis aims to determine the relationship of UCP2 gene polymorphisms rs660339 on the incidence of DM and pre-DM. SNP examination was performed using the Taqman method and analyzed by Taqman Genotyper. Hardy-Weinberg Equilibrium (HWE) analysis and odds ratios (OR) using online software from www.oege.org. Taqman Genotyper analysis results show the minor alleles in the UCP2 rs660339 gene are A and the lowest frequency is the A/A genotype. The calculation of OR shows that the G/G genotype in the UCP2 rs660339 gene has the potential to become pre-DM.