Polymorphisms in cytomegalovirus genotype in immunocompetent patients with corneal endotheliitis or iridocyclitis

Oka, Naoko; Suzuki, Takashi; Kobayashi, Takeshi; Ohashi, Yuichi

doi:10.1002/jmv.24239

Cited by 15 publications

(12 citation statements)

References 22 publications

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“…CMV viral load is also known to be associated with corneal endothelial cell damage in CMV anterior uveitis and corneal endotheliitis 17 23. Similar CMV genotypes have been detected in eyes with corneal endotheliitis and anterior uveitis;24 therefore, sufficient and sustainable reductions of CMV in the corneal endothelium and aqueous humour might be beneficial for preventing irreversible corneal endothelial dysfunction in the long term in patients with primary CMV endotheliitis and post-keratoplasty patients with CMV endotheliitis.…”

Section: Discussionmentioning

confidence: 82%

The effect of topical application of 0.15% ganciclovir gel on cytomegalovirus corneal endotheliitis

et al. 2016

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Section: Discussionmentioning

confidence: 82%

The effect of topical application of 0.15% ganciclovir gel on cytomegalovirus corneal endotheliitis

et al. 2016

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“…We found that the distribution of UL144 genotypes in our cohort ( Table 7 ) was significantly different from the distribution in Illinois, USA [ 21 ], Europe [ 23 , 26 , 27 , 38 ], Taiwan [ 7 ], and even Shenyang, China [ 5 ], but similar to the distribution in Tennessee, USA [ 19 ] and The Netherlands [ 39 ]. We noted, however, that the distribution of UL144 genotypes was similar in Japan and Taiwan [ 7 , 22 ]. Similarly, the distribution of UL146 genotypes in our cohort was significantly different from the distribution in the UK [ 29 ], Poland [ 6 ], and Japan [ 40 ], in which G2 or G1 is predominant along with G7.…”

Section: Resultsmentioning

confidence: 99%

“…The molecular epidemiology of American [ 19 ], Japanese [ 22 , 40 ], Italian [ 6 , 26 ], and Polish [ 23 ] isolates has been investigated based on UL144 and UL146. In addition, Waters et al .…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms and features of cytomegalovirus UL144 and UL146 in congenitally infected neonates with hepatic involvement

Guo

Zhang

et al. 2017

PLoS ONE

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Human cytomegalovirus is a significant agent of hepatic involvement in neonates. In this study, we investigated the polymorphisms and features of the viral genes UL144 and UL146 as well as their significance to congenital hepatic involvement. In 79 neonates with congenital cytomegalovirus infection and hepatic involvement, full length UL144 and UL146 were successfully amplified in 73.42% and 60.76% of cases, respectively. Sequencing indicated that both genes were hypervariable. Notably, UL144 genotype B was highly associated with aspartate aminotransferase (P = 0.028) and lactate dehydrogenase (P = 0.046). Similarly, UL146 genotype G1 and G13 were significantly associated with CMV IgM (P = 0.026), CMV IgG (P = 0.034), alanine aminotransferase (P = 0.019), and aspartate aminotransferase (P = 0.032). In conclusion, dominant UL144 (genotype B) and UL146 (genotype G1 and G13) genotypes are associated with elevated levels of enzymes and CMV IgM and IgG of cytomegalovirus infection.

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“…Currently, it remains unclear whether the endotheliitis type and the anterior uveitis type of CMV are caused by different CMV strains. However, Oka et al reported no significant difference in the amount of viral load or viral protein profiles for these two types of diseases [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic efficacy of real-time PCR for ocular cytomegalovirus infections

Miyazaki

Shimizu

et al. 2018

Graefes Arch Clin Exp Ophthalmol

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PurposeThe aim of this study is to determine the efficacy of quantitative real-time PCR (qPCR) and clinical characteristics to diagnose ocular cytomegalovirus (CMV) infections.MethodsThe technical factors were assessed by the outcomes of the qPCR assay at five institutions in Japan using the WHO International Standard of cytomegalovirus. The clinical factors were assessed by examining the aqueous humor samples of 197 eyes of 197 consecutive patients suspected of CMV using the receiver operating characteristics (ROCs).ResultsAll of the institutions had excellent detection efficacy, although the copy number ranged from 0.82 to 4.66 copies/IU. In the clinical samples, CMV was detected in 51 eyes, and the amount of CMV DNA was significantly higher for CMV retinitis. In corneal diseases, the amount of CMV DNA was significantly associated with frequency of recurrences and IOP elevations. The sensitivity and specificity of qPCR for the diagnosis was 90.0 and 98.7%, respectively. For the corneal and anterior uveitis types of CMV diseases, the area under the curve (AUC) of qPCR was 0.95 and 0.96, followed by frequency of recurrences with AUC of 0.89 and 0.82, and IOP elevations with AUC of 0.78 and 0.76. Unclassified cytomegalovirus detection, which did not meet diagnostic criteria of CMV corneal endotheliitis, anterior uveitis, or retinitis, was 4.6%, and it was significantly associated with corneal diseases and history of corneal transplantation.ConclusionsqPCR with standardization is specific and accurate; however, the inclusion and knowledge of the clinical characteristics improve the diagnostic efficacy.

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Polymorphisms in cytomegalovirus genotype in immunocompetent patients with corneal endotheliitis or iridocyclitis

Cited by 15 publications

References 22 publications

The effect of topical application of 0.15% ganciclovir gel on cytomegalovirus corneal endotheliitis

The effect of topical application of 0.15% ganciclovir gel on cytomegalovirus corneal endotheliitis

Polymorphisms and features of cytomegalovirus UL144 and UL146 in congenitally infected neonates with hepatic involvement

Diagnostic efficacy of real-time PCR for ocular cytomegalovirus infections

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