Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

Silva, C S; Filho, Ednaldo da Silva; Matos, Andrew Silva; Schierholt, Alex Sandro; Costa, Mônica Regina Farias; Marques, Larissa Coelho; Costa, Joana; Sales, Rodrigo Lima; Figueiro, M. R.; Marques, J. R. F.

doi:10.4238/gmr.15038720

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…In contrast, Silva et al did not find any effect of SNPs in DGAT1 on milk production traits in buffalo. The reason for this might be due to their distribution on the intron region, which is not essential for amino acid sequences on proteins (100).…”

Section: Dgat1 Role In Buffalo Milk Production Traitsmentioning

confidence: 99%

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

Khan

et al. 2021

Front. Vet. Sci.

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Milk fatty acids are essential for many dairy product productions, while intramuscular fat (IMF) is associated with the quality of meat. The triacylglycerols (TAGs) are the major components of IMF and milk fat. Therefore, understanding the polymorphisms and genes linked to fat synthesis is important for animal production. Identifying quantitative trait loci (QTLs) and genes associated with milk and meat production traits has been the objective of various mapping studies in the last decade. Consistently, the QTLs on chromosomes 14, 15, and 9 have been found to be associated with milk and meat production traits in cattle, goat, and buffalo and sheep, respectively. Diacylglycerol O-acyltransferase 1 (DGAT1) gene has been reported on chromosomes 14, 15, and 9 in cattle, goat, and buffalo and sheep, respectively. Being a key role in fat metabolism and TAG synthesis, the DGAT1 has obtained considerable attention especially in animal milk production. In addition to milk production, DGAT1 has also been a subject of interest in animal meat production. Several polymorphisms have been documented in DGAT1 in various animal species including cattle, buffalo, goat, and sheep for their association with milk production traits. In addition, the DGAT1 has also been studied for their role in meat production traits in cattle, sheep, and goat. However, very limited studies have been conducted in cattle for association of DGAT1 with meat production traits in cattle. Moreover, not a single study reported the association of DGAT1 with meat production traits in buffalo; thus, further studies are warranted to fulfill this huge gap. Keeping in view the important role of DGAT1 in animal production, the current review article was designed to highlight the major development and new insights on DGAT1 effect on milk and meat production traits in cattle, buffalo, sheep, and goat. Moreover, we have also highlighted the possible future contributions of DGAT1 for the studied species.

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Section: Dgat1 Role In Buffalo Milk Production Traitsmentioning

confidence: 99%

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

Khan

et al. 2021

Front. Vet. Sci.

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“…Conversely, the DGAT1 gene in river buffalo has received less attention so far, with information limited to gene structure (Mishra et al, 2007;Yuan et al, 2007) and polymorphism detection (Mishra et al, 2007;Yuan et al, 2007;Raut et al, 2012;Silva et al, 2016). In this respect, the K232A polymorphism has also been investigated in buffalo breeds (Tantia et al, 2006;Shi et al, 2012), which were monomorphic for the K allele.…”

Section: Introductionmentioning

confidence: 99%

Transcript analysis at DGAT1 reveals different mRNA profiles in river buffaloes with extreme phenotypes for milk fat

Cosenza

Nicolae

et al. 2017

Journal of Dairy Science

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Buffalo DGAT1 (diacylglycerol O-acyltransferase 1) was mainly investigated for the characterization of the gene itself and for the identification of the K232A polymorphism, similar to what has been accomplished in cattle, although no information has been reported so far at the mRNA level. The importance of DGAT1 for lipid metabolism led us to investigate the transcript profiles of lactating buffaloes characterized as high (9.13 ± 0.23) and low (7.94 ± 0.29) for milk fat percentage, and to explore the genetic diversity at the RNA and DNA level. A total of 336 positive clones for the DGAT1 cDNA were analyzed by PCR and chosen for sequencing according to the differences in length. The clone assembling revealed a very complex mRNA pattern with a total of 21 transcripts differently represented in the 2 groups of animals. Apart from the correct transcript (17 exons long), the skipping of exon 12 is the most significant in terms of distribution of clones with 11.6% difference between the 2 groups, whereas a totally different mRNA profile was found in approximately 12% of clones. The sequencing of genomic DNA allowed the identification of 10 polymorphic sites at the intron level, which clarify, at least partially, the genetic events behind the production of complex mRNA. Genetic diversity was found also at the exon level. The single nucleotide polymorphism c.1053C>T represents the first example of polymorphism in a coding region for the DGAT1 in the Italian Mediterranean breed. To establish whether this polymorphism is present in other buffalo breeds, a quick method based on PCR-RFLP was set up for allelic discrimination in the Italian Mediterranean and the Romanian Murrah (200 animals in total). The alleles were equally represented in the overall population, whereas the analysis of the 2 breeds showed different frequencies, likely indicating diverse genetic structure of the 2 breeds. The T allele might be considered as the ancestral condition of the DGAT1 gene, being present in the great part of the sequenced species. These data add knowledge at the transcript and genetic levels for the buffalo DGAT1 and open the opportunity for further investigation of other genes involved in milk fat metabolism for the river buffalo, including the future possibility of selecting alleles with quantitative or qualitative favorable effects (or both).

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“…The DGAT1 gene (K232A mutation) is considered to be one of the most important candidate genes for encoding acyl-CoA:diacylglycerol acyltransferase which eventually affects the milk fat content (Cases et al ., 1998; Winter et al ., 2002; Bovenhuis et al ., 2016). The majority of previous research has been done in cattle, and there are few studies of DGAT1 gene in buffalo (Yuan et al ., 2007; Cardoso et al ., 2015; Freitas et al ., 2016 a ; Parikh et al ., 2016; Silva et al ., 2016). None of these reports mentioned the identification of K232A polymorphism in the buffalo populations that they studied.…”

mentioning

confidence: 99%

“…Since the milk fat content of cow and buffalo differ considerably, DGAT1 gene expression and function in cow and buffalo could be a subject of study. Although few SNPs were identified in the buffalo DGAT1 sequence, as yet no association study has been performed to correlate DGAT1 influence in buffalo milk fat content (Yuan et al ., 2007; Cardoso et al ., 2015; Freitas et al ., 2016 a ; Silva et al ., 2016). Differences in the genomic sequence, especially from coding sequence or transcription factors binding sites in the DGAT1 gene, could help to explain the differences in milk fat quantity among these two species.…”

mentioning

confidence: 99%

The functions and mechanisms of sequence differences of DGAT1 gene on milk fat synthesis between dairy cow and buffalo

Bhattarai

Dad

Worku

et al. 2020

Journal of Dairy Research

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In this research communication we describe the DGAT1 sequence and promoter region in dairy cows and buffalo and compare the activities of DGAT1 between the two species in order to increase knowledge of the cause of milk fat variation. pGL-3 basic vectors were used to construct the reporter gene. Based on the predicted promoter region, 4 truncated plasmid vectors were constructed in cow-DGAT1 and 3 plasmid vectors in buffalo-DGAT1. Each reporter plasmid was transfected into the bovine mammary epithelial cell (BMEC), 293T cell, and CHO cells to analyze the activity using Dual-Luciferase Reporter Assay System. The results show that the region between −93 to −556 bp was essential for cow promoter activity while −84 to −590 bp was essential for buffalo promoter activity revealing these regions contain core promoter. The buffalo has higher promoter activity than cow yet it was not statistically significant. Comparison of candidate mutation K232A between cow and buffalo population revealed the presence of both the allelic population in dairy cows (lysine and alanine) however, only K (lysine) allelic amino acid was found in buffalo population. The absence of the alanine allelic population from buffalo explains the higher fat content of buffalo milk.

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Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon

Cited by 4 publications

References 16 publications

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

Transcript analysis at DGAT1 reveals different mRNA profiles in river buffaloes with extreme phenotypes for milk fat

The functions and mechanisms of sequence differences of DGAT1 gene on milk fat synthesis between dairy cow and buffalo

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