Polymorphisms in the promoter region of the bovine lactoferrin gene influence milk somatic cell score and milk production traits in Chinese Holstein cows

Mao, Yongjiang; Zhu, Xiaorui; Xing, Shiyu; Zhang, Meirong; Zhang, Huimin; Wang, Xiaolong; Karrow, Niel A.; Yang, Liguo; Yang, Zhangping

doi:10.1016/j.rvsc.2015.09.021

Cited by 13 publications

(9 citation statements)

References 43 publications

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“…The three significant SNPs located on chromosome 21 between 34.72 and 53.29 Mb are corresponding to the position between 34.80 and 53.85 Mb on chromosome 22 in bovine genome. These genomic regions reside close to previously reported QTL for LP that have been mapped by linkage study in Chinese Holstein cattle at 38.8-39.0 Mb (Mao et al, 2015).…”

Section: Assessment Of Population Structure For Gwassupporting

confidence: 75%

Determination of Potential Candidate Genes Associated with Milk Lactose in Egyptian Buffalo.

Awad¹,

Abou-Bakr²,

El-Regalaty³

et al. 2020

jwpr

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The aim of the present genome-wide association study (GWAS) was to identify single nucleotide polymorphisms (SNPs) and candidate genes associated with lactose percentage (LP) and lactose yield (LY) in Egyptian buffalo. The phenotypic dataset included 60,318 monthly measures for LP and LY from 1481 animals. A total number of 114 animals with high and low deviated performance were selected for genotyping with Axiom Buffalo Genotyping 90K Array. Genome-wide analysis was performed using a single marker regression. The GWAS revealed 32 significant and seven suggestive SNPs for LP, however; only two suggestive SNPs were identified for LY. The identified genomic regions are overlapped with previously reported QTL in different cattle breeds. In addition, novel genomic loci were detected. The identified genomic regions harbored many candidate genes with biological roles associated with milk production traits, such as TPD52 and ZBTB10 on chromosome 15; AADAT and GALNTL6 on chromosome 3 and COL8A1 and PLOD2 on chromosome 1. Our findings provide the basis to uncover the key markers and candidate genes affecting lactose traits which facilitate the exploration of the genetic mechanisms that control lactose traits variation in Egyptian buffalo.

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Section: Assessment Of Population Structure For Gwassupporting

confidence: 75%

Determination of Potential Candidate Genes Associated with Milk Lactose in Egyptian Buffalo.

Awad¹,

Abou-Bakr²,

El-Regalaty³

et al. 2020

jwpr

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“…Assim, os íons ferros são sequestrados, tornando-se indisponíveis para utilização no metabolismo microbiano, o que leva à inibição do desenvolvimento de bactérias, bolores e fungos. Outros mecanismos bactericidas da LF estão relacionados à possibilidade de aderência à parede bacteriana, ocasionando injúria celular, levando à morte ou diminuição da resistência ( Pereyra et al, 2014;Kawai et al, 2015;Mao et al, 2015;Hisaieda et al, 2016).…”

Section: Avaliação Descritiva Por Escore De Células Somáticas E Proteínasunclassified

“…De acordo com Chopra et al (2015), diferentes concentrações de LF são encontradas quando se comparam resultados de amostras de leite de vacas sadia com amostras de leite de vacas com mastite clínica e subclínica, sendo observado uma maior expressão genética do gene da LF em vacas com mastites. Devido ao importante papel desempenhado pela LF na saúde da glândula mamária, essa proteína pode ser considerada um biomarcador promissor para mastite e potencial candidata na seleção animal para resistência de mastites (Smolenski et al, 2014;Chopra et al, 2015;Mao et al, 2015).…”

Section: Avaliação Descritiva Por Escore De Células Somáticas E Proteínasunclassified

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Proteinograma do leite de vacas com mastite subclínica em função do escore de células somáticas

Alves

Sola

Mesquita

2021

RSD

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Objetivou-se, com o presente estudo, investigar a influência do aumento da CCS na expressão das proteínas do soro de leite por meio da utilização da técnica de separação de proteínas de eletroforese microfluídica em microchip (lab-on-a-chip). Foram coletadas 104 amostras de leite de vacas em duas propriedades localizadas no Estado de Goiás. As amostras foram analisadas quanto a contagem de células somáticas e eletroforese microfluídica lab-on-a-chip. Para o teste de correlação entre o perfil de proteínas e a CCS foram utilizadas 50 amostras de leite e estas foram estratificadas em cinco grupos de escores de células somáticas, sendo utilizado um delineamento inteiramente ao acaso com cinco tratamentos, avaliado por meio da estatística descritiva e representações gráficas. Verificou-se um aumento significativo no valor do componente proteína em relação aos ECS 1 e 3, 1 e 4 e 1 e 5. Também foi possível observar diferença estatística nos resultados do componente Lactose quando se comparou os ECS 1 e 3 e 3 e 4. Na avaliação descritiva da variável “Concentração” das proteínas do leite foi possível observar diferenças nos valores das concentrações em função do ECS. Foi possível observar diferença estatística para os resultados obtidos para as proteínas α-LA, quando comparados os ECS 1, 2 e 3 e o ECS 5; para a IgG, as amostras de ECS 4 e 5 apresentaram concentrações diferentes das amostras dos ECS 1, 2 e 3; e a proteína LF, apresentou resultados diferentes entre as amostras de ECS 1 e 5. Concluiu-se que proteínas de alta abundância do soro de leite tem sua concentração diminuída em função da severidade da mastite; proteínas de defesa, como Lactoferrina, Lactoperoxidase e IgG e IgM têm aumento em suas concentrações quando se comparam amostras de leite de vacas com mastite subclínica e amostras de leite de vacas sadias; as proteínas Lactoferrina e IgG constituem alvos em potencial para identificar vacas com mastite subclínica a partir de amostras de leite, podendo ser consideradas biomarcadores.

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“…The individual haplotype of each cow were inferred by software Beagle 5.1(Brian L. Browning, Washington, USA) [18]. The least-squares method and general linear model (GLM) of SPSS Ver26.0 (IBM, Armonk, New York, NY, USA) were used to analyze the associations between milk production traits /SCS and genotypes and haplotypes [18,19]. The model was as follows:…”

Section: Statistical Analysesmentioning

confidence: 99%

Polymorphisms of the ACSL1 Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows

Liang

Gao

Zhang

et al. 2020

Animals

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Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (ACSL1) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in ACSL1 on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine ACSL1 gene, and six SNPs (5’UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS (p < 0.05). Among these SNPs, SNP 5’UTR-g.20523C>G showed an extremely significant effect on PC and SCS (p < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS (p < 0.01), and also TDMY (p < 0.05). The SNP g.35651G>A had a statistically significant effect on PC (p < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS (p < 0.01) and significantly influenced PC (p < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS (p < 0.05). In summary, the pleiotropic effects of bovine ACSL1 for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows.

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Polymorphisms in the promoter region of the bovine lactoferrin gene influence milk somatic cell score and milk production traits in Chinese Holstein cows

Cited by 13 publications

References 43 publications

Determination of Potential Candidate Genes Associated with Milk Lactose in Egyptian Buffalo.

Determination of Potential Candidate Genes Associated with Milk Lactose in Egyptian Buffalo.

Proteinograma do leite de vacas com mastite subclínica em função do escore de células somáticas

Polymorphisms of the ACSL1 Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows

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