Polymorphisms of endothelial nitric oxide synthase and hypoxia-inducible factor 1 alpha genes play a role in susceptibility to coronary artery disease

Abstract

Objective: Genetics is a known factor in the susceptibility to cardiovascular disease. In this study, polymorphisms of endothelial nitric oxide synthase (eNOS) (T876C and G894T) and hypoxiainducible factor 1 (HIF1) (rs10873142 and rs41508050) in coronary artery disease were compared with healthy subjects, and the relationship between these mutations and risk of coronary artery disease (CAD) was assessed.
 Methods: Blood samples were collected from 138 patients with obstructive CAD and 115 healthy subjects. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), Griess test, and enzyme-linked immunosorbent assay (ELISA) were used to measure serum nitric oxide (NO) and paraoxonase antioxidant. Independent t-test was used to examine the relationship between variables.
 Results: There was no significant difference between genotype frequency in healthy subjects and patients for eNOS and HIF1 alpha polymorphisms. There was no significant difference between these polymorphisms and age, sex, hypertension and lipidemia. Furthermore, the difference in mean serum levels of NO in different genotypes of the G894T gene was not significant between the patient and control groups. However, the difference in mean serum NO levels in different genotypes of the T786C gene was significant between the patient and control groups such that minimum and maximum serum NO levels were observed in individuals bearing TT and TC genotypes, respectively. The difference in mean serum NO levels was higher in patients than in controls, and was statistically significant.
 Conclusions: The results suggest that the T876C polymorphism could be associated with low serum NO level and implicated as a risk factor for developing CAD. Furthermore, our findings indicate that genetics may not be involved in susceptibility to CAD; however, further comprehensive studies are required.

Assessment of Hypoxia-Inducible Factor-1? and Vascular Endothelial Growth Factor Levels in Type 2 Diabetes Mellitus Patients in North Sumatera, Indonesia

“…15 HIF-1α is a key oxygen sensor and mediator of angiogenesis, 16 and it acts by binding a hypoxia-response element in the VEGF promoter to up-regulate VEGF expression. 17 Other researchers found no significant relationship between HIF-1α, FBS, and HbA1c (p > 0.005) in type 2 diabetes mellitus with or without malignancy (p > 0.005). 18 However, the results of this study showed that both groups had relatively small levels of HIF-1α and also high levels in the serum.…”

Vasospastic angina: pathophysiology and clinical significance

“…Интракоронарное введение антиоксидантов (витаминов С, Е, глутатиона) улучшает функцию эндотелия [2,5]. У женщин с ВС в постменопаузе эстрогены усиливают антиокислительный потенциал, активность эндотелиальной NO-синтазы и подавляют СКА [27]. ВС ассоциируется также с полиморфизмом генов, кодирующих синтез медиаторов, обладающих антиокислительным действием, включая ALDH2, параоксоназу 1, стромелизин-1, интерлейкин-6 и фосфолипазу Cδ1 [2,3,20].…”