Polymorphisms of FLII implicate gene expressions and growth traits in Chinese cattle

Liu, Mei; Liu, Min; Li, Bo; Zhou, Yang; Huang, Yongzhen; Lan, Xianyong; Qu, Weidong; Qi, Xingshan; Bai, Yueyu; Chen, Hong

doi:10.1016/j.mcp.2016.07.005

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…Forty DNA samples were randomly selected from each cattle breed, to construct for DNA pools of four breeds [ 19 ]. PCR reactions were carried out using a Touchdown PCR System Thermal Cycler Dice (TaKaRa, Dalian, China) and a PCR protocol which was described in a previous study [ 20 ]. DNA pool sequencing for the PCR productions using a Sanger sequencing strategy was applied to screen the genetic variations.…”

Section: Methodsmentioning

confidence: 99%

“…The PCR reactions were carried out in a 25 μL volume which contained 1 μL cattle genomic DNA (50 ng/μL), 0.5 μL each primer (10 μmol/L), 12.5 μL 2 × Taq PCR MasterMix (Taq DNA polymerase, Mg 2+ , dNTPs, et al), and ddH 2 O. The PCR tests were carried out with the PCR System Thermal Cycler Dice (TaKaRa, Dalian, China), with the following amplification protocol: 94 °C for 5 min; followed by 35 cycles of 94 °C for 30 s, annealing at selected temperatures ( Table 1 ) for 30 s, and extension at 72 °C for a 30 s; and a final extension at 72 °C for 10 min [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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KAT2B Gene Polymorphisms Are Associated with Body Measure Traits in Four Chinese Cattle Breeds

Lin

Chen

et al. 2022

Animals

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Identifying molecular markers related to growth characteristics or meat quality is significant for improving beef cattle breeds. K(lysine) acetyltransferase 2B (KAT2B) is a transcriptional co-activator regulating the acetylation modification of histones, which may be involved in the development and metabolism of muscle and adipose. However, investigations of KAT2B genetic variations in Chinese native cattle are still limited. This study aimed to identify crucial single nucleotide polymorphisms (SNPs) influencing the body measurements of Chinese native cattle. Biological evolution and conservation analysis showed that KAT2B was highly conserved among the ruminants. By qPCR assay, KAT2B gene expression was found to be spatiotemporally specific in bovine tissues such as adipose and liver. By the RFLP-PCR method, three SNPs of KAT2B (g.T61908C, g.T62131C, and g.C73406T) were identified in 827 individuals of four Chinese cattle breeds, including Qinchuan (n = 658), Fu (n = 52), Yak (n = 48), and Chaidam (n = 69) cattle. Association analysis between these KAT2B polymorphisms and the body measurements of Chinese native cattle revealed significant observations. The genetic effects of g.T61908C, g.T62131C, and g.C73406T on the associated phenotypes were illustrated in each breed. In Qinchuan cattle, g.T62131C was significantly associated with better body height, chest width, hip width, and withers height, for which TC and/or TT were the advantageous genotype. In Fu cattle, TT genotype of g.T61908C was associated to better body length, while individuals with TT or CC of g.T62131C showed higher circumference of cannon bone than those with TC genotype. In Yak, individuals with TT genotype of g.C73406T had heavier body weight. In Chaidam cattle, TC genotype of g.C73406T was associated to superior body weight, while CC genotype of g.C73406T was associated to superior chest girth and circumference of cannon bone. These findings suggest that KAT2B gene polymorphisms can be used as the molecular markers for the early molecular marker-assisted selection in beef cattle breeding programs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

KAT2B Gene Polymorphisms Are Associated with Body Measure Traits in Four Chinese Cattle Breeds

Lin

Chen

et al. 2022

Animals

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“…Genetic characterisation of a number of Chinese cattle breeds, which historically exhibit small body size and low intramuscular fat content compared to other meat production breeds, shows that three polymorphisms in Flii are associated with increased body mass, height and length, as well as chest girth. Moreover, these polymorphisms; CT (rs41910826), TT (rs444484913), and CA (rs522737248) were associated with increased PPARγ in adult adipose as well as increased Flii in fetal muscle (Liu et al, 2016).…”

Section: Transcriptional Regulation and Metabolismmentioning

confidence: 99%

“…Flii has been shown to act as a repressor of the transcriptional activity of the Peroxisome Proliferator-Activated Receptor γ (PPARγ) and its overexpression suppresses adipogenesis (Choi et al, 2015). Flii is expressed more highly in adult bovine adipose tissue compared to fetal bovine adipose tissue (Zhou et al, 2014), with Flii expression increasing in differentiated adipocytes compared with preadipocytes (Liu et al, 2016). In the absence of ligand, the LXXLL motif within the LRR domain of Flii binds directly to the DNA binding domain of the receptor to prevent PPARγ receptor occupancy at the promotor of target genes as well as blocking the interaction between PPARγ and retinoid X receptor α (RXRα) (Choi et al, 2015).…”

Section: Transcriptional Regulation and Metabolismmentioning

confidence: 99%

Multifunctional Roles of the Actin-Binding Protein Flightless I in Inflammation, Cancer and Wound Healing

Strudwick

Cowin

2020

Front. Cell Dev. Biol.

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Flightless I is an actin-binding member of the gelsolin family of actin-remodeling proteins that inhibits actin polymerization but does not possess actin severing ability. Flightless I functions as a regulator of many cellular processes including proliferation, differentiation, apoptosis, and migration all of which are important for many physiological processes including wound repair, cancer progression and inflammation. More than simply facilitating cytoskeletal rearrangements, Flightless I has other important roles in the regulation of gene transcription within the nucleus where it interacts with nuclear hormone receptors to modulate cellular activities. In conjunction with key binding partners Leucine rich repeat in the Flightless I interaction proteins (LRRFIP)1/2, Flightless I acts both synergistically and competitively to regulate a wide range of cellular signaling including interacting with two of the most important inflammatory pathways, the NLRP3 inflammasome and the MyD88-TLR4 pathways. In this review we outline the current knowledge about this important cytoskeletal protein and describe its many functions across a range of health conditions and pathologies. We provide perspectives for future development of Flightless I as a potential target for clinical translation and insights into potential therapeutic approaches to manipulate Flightless I functions.

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“…Finally, the CNV results were summarized and displayed by Prism software (version 5.0.0). In the analysis of gene expression profiles, internal reference Emerin gene (EMD) and lipoprotein receptor-related protein 10 gene (LRP10) were used as reference internal genes for the expression level identifications following the study of Liu et al [47] and Saremi et al [48], respectively. The gene expression levels were also The amplification efficiencies of primers were tested by genomic DNA with PCR products concentration gradients that were 500, 100, 20, 4 and 0.8 ng.…”

Section: Identification Of Cnv and Expression Levels For Gbp6mentioning

confidence: 99%

Copy Number Variations and Expression Levels of Guanylate-Binding Protein 6 Gene Associated with Growth Traits of Chinese Cattle

Hao

Wang

Thomsen

et al. 2020

Animals

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Association studies have indicated profound effects of copy number variations (CNVs) on various phenotypes in different species. In this study, we identified the CNV distributions and expression levels of guanylate-binding protein 6 (GBP6) associated with the growth traits of Chinese cattle. The results showed that the phenotypic values of body size and weight of Xianan (XN) cattle were higher than those of Nanyang (NY) cattle. The medium CNV types were mostly identified in the XN and NY breeds, but their CNV distributions were significantly different (adjusted p < 0.05). The association analysis revealed that the body weight, cannon circumference and chest circumference of XN cattle had significantly different values in different CNV types (p < 0.05), with CNV gain types (Log22−ΔΔCt > 0.5) displaying superior phenotypic values. We also found that transcription levels varied in different tissues (p < 0.001) and the CNV gain types showed the highest relative gene expression levels in the muscle tissue, consistent with the highest phenotypic values of body weight and cannon circumference among the three CNV types. Consequently, our results suggested that CNV gain types of GBP6 could be used as the candidate markers in the cattle-breeding program for growth traits.

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Polymorphisms of FLII implicate gene expressions and growth traits in Chinese cattle

Cited by 13 publications

References 28 publications

KAT2B Gene Polymorphisms Are Associated with Body Measure Traits in Four Chinese Cattle Breeds

KAT2B Gene Polymorphisms Are Associated with Body Measure Traits in Four Chinese Cattle Breeds

Multifunctional Roles of the Actin-Binding Protein Flightless I in Inflammation, Cancer and Wound Healing

Copy Number Variations and Expression Levels of Guanylate-Binding Protein 6 Gene Associated with Growth Traits of Chinese Cattle

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